WIPI3_XENLA
ID WIPI3_XENLA Reviewed; 344 AA.
AC Q68F45;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=WD repeat domain phosphoinositide-interacting protein 3;
DE Short=WIPI-3;
DE AltName: Full=WD repeat-containing protein 45-like;
DE Short=WDR45-like protein;
DE AltName: Full=WD repeat-containing protein 45B;
GN Name=wdr45b; Synonyms=wdr45l, wipi3;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the autophagy machinery that controls the major
CC intracellular degradation process by which cytoplasmic materials are
CC packaged into autophagosomes and delivered to lysosomes for
CC degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and
CC other phosphoinositides including PtdIns(3,5)P2, forming on membranes
CC of the endoplasmic reticulum upon activation of the upstream ULK1 and
CC PI3 kinases and is recruited at phagophore assembly sites where it
CC regulates the elongation of nascent phagophores downstream of WIPI2.
CC {ECO:0000250|UniProtKB:Q5MNZ6}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure
CC {ECO:0000250|UniProtKB:Q5MNZ6}. Lysosome
CC {ECO:0000250|UniProtKB:Q5MNZ6}.
CC -!- DOMAIN: The L/FRRG motif is required for recruitment to PtdIns3P.
CC {ECO:0000250|UniProtKB:Q9Y4P8}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; BC080000; AAH80000.1; -; mRNA.
DR RefSeq; NP_001087486.1; NM_001094017.1.
DR AlphaFoldDB; Q68F45; -.
DR SMR; Q68F45; -.
DR MaxQB; Q68F45; -.
DR DNASU; 447310; -.
DR GeneID; 447310; -.
DR KEGG; xla:447310; -.
DR CTD; 447310; -.
DR Xenbase; XB-GENE-6254013; wdr45b.S.
DR OMA; WSFCKFQ; -.
DR OrthoDB; 966922at2759; -.
DR Proteomes; UP000186698; Chromosome 9_10S.
DR Bgee; 447310; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; ISS:UniProtKB.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; ISS:UniProtKB.
DR GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR GO; GO:0009267; P:cellular response to starvation; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Lipid-binding; Lysosome; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..344
FT /note="WD repeat domain phosphoinositide-interacting
FT protein 3"
FT /id="PRO_0000051450"
FT REPEAT 183..223
FT /note="WD 1"
FT REPEAT 228..267
FT /note="WD 2"
FT MOTIF 224..227
FT /note="L/FRRG motif"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4P8"
SQ SEQUENCE 344 AA; 38182 MW; 56A91E5CAFEB570F CRC64;
MNLLPSNPHG NGLLYSGFNQ DHGCFACGME NGFRVYNTDP LKEKEKQEFL EGGVSYVEML
FRCNYLALVG GGKKPKYPPN KVMIWDDLKK KTVIEIEFST EVKAVKLRRD RIVVVLDSMI
KVFTFTHNPH QLHVFETCYN PKGLCVLCPN SNNSLLAFPG AHTGHVQIVD LASTEKPPVD
IPAHEGILSC IALNLQGTRI ATASEKGTLI RIFDTSSGHL IQELRRGSQA ANIYCINFNE
DASLICVSSD HGTVHIFAAE DPKRNKQSSL ASASFLPKYF SSKWSFSKFQ VPSGSPCICA
FGTEPNSVIA ICADGSYYKF QFNPKGECTR DVYAQFLEMT DDKL
