WIPI4_DANRE
ID WIPI4_DANRE Reviewed; 358 AA.
AC Q7ZUX3; Q6NY84; Q6TGY9;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=WD repeat domain phosphoinositide-interacting protein 4;
DE Short=WIPI-4;
DE AltName: Full=WD repeat-containing protein 45;
GN Name=wdr45; Synonyms=wipi4;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo, and Kidney;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the autophagy machinery that controls the major
CC intracellular degradation process by which cytoplasmic materials are
CC packaged into autophagosomes and delivered to lysosomes for degradation
CC (By similarity). Binds phosphatidylinositol 3-phosphate (PtdIns3P) (By
CC similarity). {ECO:0000250|UniProtKB:Q9Y484}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure
CC {ECO:0000250|UniProtKB:Q9Y484}.
CC -!- DOMAIN: The L/FRRG motif is required for recruitment to PtdIns3P.
CC {ECO:0000250|UniProtKB:Q9Y4P8}.
CC -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR EMBL; AY398367; AAQ97800.1; -; mRNA.
DR EMBL; BC046090; AAH46090.1; -; mRNA.
DR EMBL; BC066700; AAH66700.1; -; mRNA.
DR RefSeq; NP_956525.1; NM_200231.1.
DR RefSeq; XP_005169291.1; XM_005169234.3.
DR AlphaFoldDB; Q7ZUX3; -.
DR SMR; Q7ZUX3; -.
DR STRING; 7955.ENSDARP00000108156; -.
DR PaxDb; Q7ZUX3; -.
DR GeneID; 393200; -.
DR KEGG; dre:393200; -.
DR CTD; 11152; -.
DR ZFIN; ZDB-GENE-040426-990; wdr45.
DR eggNOG; KOG2111; Eukaryota.
DR InParanoid; Q7ZUX3; -.
DR OrthoDB; 966922at2759; -.
DR PhylomeDB; Q7ZUX3; -.
DR TreeFam; TF314859; -.
DR Reactome; R-DRE-1632852; Macroautophagy.
DR PRO; PR:Q7ZUX3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; ISS:UniProtKB.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; ISS:UniProtKB.
DR GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; ISS:UniProtKB.
DR GO; GO:2000786; P:positive regulation of autophagosome assembly; ISS:UniProtKB.
DR GO; GO:0006497; P:protein lipidation; IBA:GO_Central.
DR GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR032910; WIPI4.
DR PANTHER; PTHR11227:SF44; PTHR11227:SF44; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Lipid-binding; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..358
FT /note="WD repeat domain phosphoinositide-interacting
FT protein 4"
FT /id="PRO_0000051455"
FT REPEAT 2..40
FT /note="WD 1"
FT REPEAT 188..228
FT /note="WD 2"
FT REPEAT 233..272
FT /note="WD 3"
FT MOTIF 229..232
FT /note="L/FRRG motif"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4P8"
FT CONFLICT 131
FT /note="V -> A (in Ref. 1; AAQ97800)"
FT /evidence="ECO:0000305"
FT CONFLICT 180
FT /note="S -> F (in Ref. 1; AAQ97800)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="A -> E (in Ref. 2; AAH66700)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 358 AA; 39641 MW; 3A3DAE86FEC01FD1 CRC64;
MAQQRGVNSL QFNQDQSCFC CAMETGVRIY NVEPLMEKGH LDHEQVGSIA LCSMLHRSNL
LAVVGGGVNP KFSEISVLIW DDAREVRDPK DKLVLEFTFT KPVLAVRMRH DKIIIILKNR
IYVYSFPDNP VKLFEFDTRD NPKGLCDLCP SLEKQLLVFP GHKCGSLQLV DLSNTKPGTS
SAPFTINAHQ SEIACLALNQ PGSVVASASR KGTLIRLFDT TTRDKLVELR RGTDPATLYC
INFSHDSSFL CASSDKGTVH IFALKDTKLN RRSALARVGK VGPVIGQYVD SQWSLANFTV
PAECACICAF GKNTSKNVNS VIAICVDGTF HKYVFTPDGN CNREAFDVYL DICDDDDF
