ID   WLSA_XENLA              Reviewed;         541 AA.
AC   Q66IZ4;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Protein wntless homolog A;
DE   AltName: Full=Integral membrane protein GPR177-A;
DE   Flags: Precursor;
GN   Name=wls-a; Synonyms=gpr177-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for a subset of Wnt-dependent developmental
CC       processes, in particular, eye and pronephros development. Regulates the
CC       secretion of wnt4, which is required for eye development (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC       pass membrane protein {ECO:0000250}. Cytoplasmic vesicle membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the wntless family. {ECO:0000305}.
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DR   EMBL; BC081130; AAH81130.1; -; mRNA.
DR   RefSeq; NP_001087716.1; NM_001094247.1.
DR   AlphaFoldDB; Q66IZ4; -.
DR   SMR; Q66IZ4; -.
DR   MaxQB; Q66IZ4; -.
DR   DNASU; 447540; -.
DR   GeneID; 447540; -.
DR   KEGG; xla:447540; -.
DR   CTD; 447540; -.
DR   Xenbase; XB-GENE-6253761; wls.S.
DR   OMA; YWRHLSA; -.
DR   OrthoDB; 418201at2759; -.
DR   Proteomes; UP000186698; Chromosome 4S.
DR   Bgee; 447540; Expressed in internal ear and 19 other tissues.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0017147; F:Wnt-protein binding; IEA:InterPro.
DR   GO; GO:0061357; P:positive regulation of Wnt protein secretion; ISS:ParkinsonsUK-UCL.
DR   GO; GO:0030177; P:positive regulation of Wnt signaling pathway; ISS:ParkinsonsUK-UCL.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR009551; Wntless.
DR   PANTHER; PTHR13449; PTHR13449; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Developmental protein; Golgi apparatus; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW   Wnt signaling pathway.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..541
FT                   /note="Protein wntless homolog A"
FT                   /id="PRO_0000271782"
FT   TOPO_DOM        29..232
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        233..253
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        254..268
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        269..289
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        290..303
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        325..331
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        353..379
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        401..431
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..452
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        453..471
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        493..541
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   541 AA;  61912 MW;  6CFDF6CA449B36B4 CRC64;
     MAGAIIENMS TKKLCMVGVA LLLLQVLAFL VGGLIAPKPT SYVNPVAMKC VDVRKNHRSS
     KWLMPWGTEP CKSIQSFDEA ANRMIEANDI VFAAHIPNSQ FEMSPWFQFM LVVLQLDIAF
     KLNNYIEENS MVTLDVSVAY RDDLKEEWKE LASSVEQRKL NCILPVEKTL ANEGRHYDCD
     VIPLMELGSV SHKYYLFNIR LPVNERKKAN IGIGEIRDLH VVSIFQNGGF TMVWFAMKTF
     LTPSIIIIMI WYWRRITMMT RSPVLLEKVI FALGFSMTFI NIPVEWFSIG YDWTWMLLFG
     DIRQGIFYAM LLSFWIIFCG EHMMDQTERN RISVYWKQVG PIAFGSCCLF IFDMCERGVQ
     LKNPFYSIWT TDVGAEIAMA FIIVAGICAC LYFLFLCFMV YQVFRNISGK QSNLPAMTKA
     RRLHYEGLIF RFKFLMIITL ACAALTIVFF ITTQITEGNW KLGDLSIELN SAFFTGVYGM
     WNLYVFALMF LYAPSHKHYG DGQSNDGAGM SSGEELQLTT TITHIDGPTE VYRLAGKEAQ
     E