WLS_RAT
ID WLS_RAT Reviewed; 541 AA.
AC Q6P689;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein wntless homolog;
DE AltName: Full=Integral membrane protein GPR177;
DE AltName: Full=Protein evenness interrupted homolog;
DE Short=EVI;
GN Name=Wls; Synonyms=Gpr177;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Wistar;
RX PubMed=15326124; DOI=10.1167/iovs.04-0302;
RA Ahmed F., Torrado M., Zinovieva R.D., Senatorov V.V., Wistow G.,
RA Tomarev S.I.;
RT "Gene expression profile of the rat eye iridocorneal angle: NEIBank
RT expressed sequence tag analysis.";
RL Invest. Ophthalmol. Vis. Sci. 45:3081-3090(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=20652957; DOI=10.1002/dvdy.22369;
RA Jin J., Morse M., Frey C., Petko J., Levenson R.;
RT "Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt-
RT transport protein, in rat tissues, zebrafish embryos, and cultured human
RT cells.";
RL Dev. Dyn. 239:2426-2434(2010).
CC -!- FUNCTION: Regulates Wnt proteins sorting and secretion in a feedback
CC regulatory mechanism. This reciprocal interaction plays a key role in
CC the regulation of expression, subcellular location, binding and
CC organelle-specific association of Wnt proteins. Also plays an important
CC role in establishment of the anterior-posterior body axis formation
CC during development (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q6P689; P33535: Oprm1; NbExp=2; IntAct=EBI-6113235, EBI-4392569;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q5T9L3}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q5T9L3}. Cytoplasmic vesicle membrane
CC {ECO:0000250|UniProtKB:Q5T9L3}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q5T9L3}. Cell membrane
CC {ECO:0000250|UniProtKB:Q5T9L3}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q5T9L3}; Multi-pass membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q5T9L3};
CC Multi-pass membrane protein {ECO:0000255}. Early endosome membrane
CC {ECO:0000250|UniProtKB:Q5T9L3}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the brain, skeletal muscle, heart
CC muscle, lung, gut, liver, and kidney (at protein level)
CC (PubMed:20652957). In the brain, expressed in the cortex, striatum,
CC ventral tegmentum, nucleus accumbens and to a lesser extent in the
CC Purkinjie cells in the cerebellum (PubMed:20652957). Expressed in eye
CC iridocorneal angle (PubMed:15326124). {ECO:0000269|PubMed:15326124,
CC ECO:0000269|PubMed:20652957}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:20652957}.
CC -!- SIMILARITY: Belongs to the wntless family. {ECO:0000305}.
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DR EMBL; AY569010; AAS75313.1; -; mRNA.
DR EMBL; BC062391; AAH62391.1; -; mRNA.
DR RefSeq; NP_001078822.1; NM_001085353.1.
DR RefSeq; NP_955440.1; NM_199408.2.
DR AlphaFoldDB; Q6P689; -.
DR SMR; Q6P689; -.
DR IntAct; Q6P689; 1.
DR STRING; 10116.ENSRNOP00000052120; -.
DR PhosphoSitePlus; Q6P689; -.
DR jPOST; Q6P689; -.
DR PaxDb; Q6P689; -.
DR Ensembl; ENSRNOT00000055245; ENSRNOP00000052120; ENSRNOG00000036816.
DR GeneID; 362065; -.
DR KEGG; rno:362065; -.
DR CTD; 79971; -.
DR RGD; 735139; Wls.
DR eggNOG; ENOG502QSE2; Eukaryota.
DR GeneTree; ENSGT00390000005897; -.
DR HOGENOM; CLU_022911_0_0_1; -.
DR InParanoid; Q6P689; -.
DR OMA; YWRHLSA; -.
DR OrthoDB; 418201at2759; -.
DR PhylomeDB; Q6P689; -.
DR Reactome; R-RNO-3238698; WNT ligand biogenesis and trafficking.
DR PRO; PR:Q6P689; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000036816; Expressed in adult mammalian kidney and 19 other tissues.
DR ExpressionAtlas; Q6P689; baseline and differential.
DR Genevisible; Q6P689; RN.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
DR GO; GO:0032839; C:dendrite cytoplasm; IDA:RGD.
DR GO; GO:0032590; C:dendrite membrane; IDA:RGD.
DR GO; GO:0005769; C:early endosome; ISO:RGD.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031301; C:integral component of organelle membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0005802; C:trans-Golgi network; ISO:RGD.
DR GO; GO:0031852; F:mu-type opioid receptor binding; IPI:RGD.
DR GO; GO:0017147; F:Wnt-protein binding; ISO:RGD.
DR GO; GO:0009948; P:anterior/posterior axis specification; ISS:UniProtKB.
DR GO; GO:0071529; P:cementum mineralization; ISO:RGD.
DR GO; GO:0031017; P:exocrine pancreas development; ISO:RGD.
DR GO; GO:0030902; P:hindbrain development; ISO:RGD.
DR GO; GO:0006886; P:intracellular protein transport; ISO:RGD.
DR GO; GO:0001707; P:mesoderm formation; ISO:RGD.
DR GO; GO:0030901; P:midbrain development; ISO:RGD.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0061357; P:positive regulation of Wnt protein secretion; ISS:ParkinsonsUK-UCL.
DR GO; GO:0030177; P:positive regulation of Wnt signaling pathway; ISS:ParkinsonsUK-UCL.
DR GO; GO:0061355; P:Wnt protein secretion; ISO:RGD.
DR GO; GO:0016055; P:Wnt signaling pathway; ISS:UniProtKB.
DR InterPro; IPR009551; Wntless.
DR PANTHER; PTHR13449; PTHR13449; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasmic vesicle; Developmental protein;
KW Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix;
KW Wnt signaling pathway.
FT CHAIN 1..541
FT /note="Protein wntless homolog"
FT /id="PRO_0000271780"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 16..36
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..232
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 233..253
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..268
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 269..289
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..303
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 304..324
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 332..352
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..380
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 381..401
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 402..431
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 432..452
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 453..471
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT TRANSMEM 472..492
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 493..541
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q5T9L3"
FT REGION 101..202
FT /note="Interaction with Wnt proteins"
SQ SEQUENCE 541 AA; 62202 MW; 58DCC18634B86668 CRC64;
MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVSYVAAKC VDVRKNHHKT
RWLMPWGPNK CNKINDFEEA IPREIEANDI VFSVHIPLPS MEMSPWFQFM LFILQIDIAF
KLNNQIRENA EVSMDVSLGY RDDMFSEWTE MAHERVPRKL RCTFTSPKTP EHEGRHYECD
VLPFMEIGSV AHKYYLLNIR LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF
LTPSIFIIMV WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG
DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF IFDMCERGVQ
LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV FQVFRNISGK QSSLPAMSKV
RRLHYEGLIF RFKFLMLITL ACAAMTVIFF IVSQVTEGHW KWGGVTVQVS SAFFTGIYGM
WNLYVFALMF LYAPSHKNYG EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ
E
