WN10A_MOUSE
ID WN10A_MOUSE Reviewed; 417 AA.
AC P70701;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Protein Wnt-10a;
DE Flags: Precursor;
GN Name=Wnt10a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=8875992;
RA Wang J., Shackleford G.M.;
RT "Murine Wnt10a and Wnt10b: cloning and expression in developing limbs, face
RT and skin of embryos and in adults.";
RL Oncogene 13:1537-1544(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=28589954; DOI=10.1038/ncomms15397;
RA Xu M., Horrell J., Snitow M., Cui J., Gochnauer H., Syrett C.M.,
RA Kallish S., Seykora J.T., Liu F., Gaillard D., Katz J.P., Kaestner K.H.,
RA Levin B., Mansfield C., Douglas J.E., Cowart B.J., Tordoff M., Liu F.,
RA Zhu X., Barlow L.A., Rubin A.I., McGrath J.A., Morrisey E.E., Chu E.Y.,
RA Millar S.E.;
RT "WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell
RT proliferation and KLF4-mediated differentiation.";
RL Nat. Commun. 8:15397-15397(2017).
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors (Probable). Functions in the canonical
CC Wnt/beta-catenin signaling pathway. Plays a role in normal ectoderm
CC development. Required for normal tooth development. Required for normal
CC postnatal development and maintenance of tongue papillae and sweat
CC ducts. Required for normal proliferation of basal cells in tongue
CC filiform papillae, plantar epithelium and sweat ducts. Required for
CC normal expression of keratins in tongue papillae. Required for normal
CC expression of KRT9 in foot plant epithelium. Required for normal hair
CC follicle function. {ECO:0000269|PubMed:28589954, ECO:0000305}.
CC -!- SUBUNIT: Forms a soluble 1:1 complex with AFM; this prevents
CC oligomerization and is required for prolonged biological activity. The
CC complex with AFM may represent the physiological form in body fluids.
CC {ECO:0000250|UniProtKB:Q9GZT5}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250|UniProtKB:Q9GZT5}. Secreted
CC {ECO:0000250|UniProtKB:Q9GZT5}.
CC -!- TISSUE SPECIFICITY: Detected in foot plant epidermis, footpad
CC epidermis, haired skin epidermis. Detected in adult epithelia,
CC including filiform and fungiform papillae and sweat ducts. Detected in
CC sweat gland myoepithelial cells, but not in sweat gland mesenchyme.
CC {ECO:0000269|PubMed:28589954}.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC inhibition. {ECO:0000250|UniProtKB:P27467,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR EMBL; U61969; AAB08085.1; -; mRNA.
DR EMBL; BC014737; AAH14737.1; -; mRNA.
DR CCDS; CCDS15057.1; -.
DR PIR; B59392; B59392.
DR RefSeq; NP_033544.1; NM_009518.2.
DR AlphaFoldDB; P70701; -.
DR SMR; P70701; -.
DR IntAct; P70701; 1.
DR STRING; 10090.ENSMUSP00000006718; -.
DR GlyGen; P70701; 2 sites.
DR iPTMnet; P70701; -.
DR PhosphoSitePlus; P70701; -.
DR PaxDb; P70701; -.
DR PRIDE; P70701; -.
DR ProteomicsDB; 299986; -.
DR Antibodypedia; 2463; 209 antibodies from 35 providers.
DR DNASU; 22409; -.
DR Ensembl; ENSMUST00000006718; ENSMUSP00000006718; ENSMUSG00000026167.
DR GeneID; 22409; -.
DR KEGG; mmu:22409; -.
DR UCSC; uc007bnf.2; mouse.
DR CTD; 80326; -.
DR MGI; MGI:108071; Wnt10a.
DR VEuPathDB; HostDB:ENSMUSG00000026167; -.
DR eggNOG; KOG3913; Eukaryota.
DR GeneTree; ENSGT00940000160299; -.
DR HOGENOM; CLU_033039_1_3_1; -.
DR InParanoid; P70701; -.
DR OMA; MKIYANQ; -.
DR OrthoDB; 1241694at2759; -.
DR PhylomeDB; P70701; -.
DR TreeFam; TF105310; -.
DR Reactome; R-MMU-3238698; WNT ligand biogenesis and trafficking.
DR BioGRID-ORCS; 22409; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Wnt10a; mouse.
DR PRO; PR:P70701; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P70701; protein.
DR Bgee; ENSMUSG00000026167; Expressed in calcareous tooth and 112 other tissues.
DR ExpressionAtlas; P70701; baseline and differential.
DR Genevisible; P70701; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR GO; GO:0048018; F:receptor ligand activity; ISO:MGI.
DR GO; GO:0005102; F:signaling receptor binding; TAS:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; ISO:MGI.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; TAS:MGI.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEA:Ensembl.
DR GO; GO:0048730; P:epidermis morphogenesis; ISO:MGI.
DR GO; GO:0001942; P:hair follicle development; ISO:MGI.
DR GO; GO:0031069; P:hair follicle morphogenesis; ISO:MGI.
DR GO; GO:0014033; P:neural crest cell differentiation; IDA:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0042476; P:odontogenesis; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:MGI.
DR GO; GO:0042487; P:regulation of odontogenesis of dentin-containing tooth; IDA:MGI.
DR GO; GO:0048733; P:sebaceous gland development; ISO:MGI.
DR GO; GO:0007165; P:signal transduction; TAS:MGI.
DR GO; GO:0043588; P:skin development; ISO:MGI.
DR GO; GO:0043586; P:tongue development; ISO:MGI.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR013302; Wnt10.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01893; WNT10PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Phosphoprotein; Reference proteome; Secreted; Signal;
KW Wnt signaling pathway.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..417
FT /note="Protein Wnt-10a"
FT /id="PRO_0000041461"
FT REGION 300..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 59
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O00744"
FT LIPID 268
FT /note="O-palmitoleoyl serine; by PORCN"
FT /evidence="ECO:0000250|UniProtKB:P56704"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 363
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 96..107
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 149..157
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 159..214
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 262..276
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 264..271
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 346..377
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 362..372
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 376..416
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 392..407
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 394..404
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 399..400
FT /evidence="ECO:0000250|UniProtKB:P28026"
SQ SEQUENCE 417 AA; 46454 MW; 048DEF5E4CB94284 CRC64;
MGSAHPRPWL RLPQGPQPRP EFWALLFFLL LLAAAVPRSA PNDILGLRLP PEPVLNANTV
CLTLPGLSRR QMEVCVRHPD VAASAIQGIQ IAIHECQHQF RDQRWNCSSL ETRNKVPYES
PIFSRGFRES AFAYAIAAAG VVHAVSNACA LGKLKACGCD ASRRGDEEAF RRKLHRLQLD
ALQRGKGLSH GVPEHPAILP ASPGLQDSWE WGGCSPDVGF GERFSKDFLD SREPHRDIHA
RMRLHNNRVG RQAVMENMRR KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RNRFHRATLI
RPHNRNGGQL EPGPAGAPSP APGTPGLRRR ASHSDLVYFE KSPDFCEREP RLDSAGTVGR
LCNKSSTGPD GCGSMCCGRG HNILRQTRSE RCHCRFHWCC FVVCEECRIT EWVSVCK
