WNK1_ORYSJ
ID WNK1_ORYSJ Reviewed; 704 AA.
AC Q0D598; A0A0P0X875; Q6ZL25; Q6ZL26;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Probable serine/threonine-protein kinase WNK1;
DE Short=OsWNK1;
DE EC=2.7.11.1;
DE AltName: Full=Mitogen-activated protein kinase kinase 1;
DE AltName: Full=Protein kinase with no lysine 1;
GN Name=WNK1; Synonyms=MEK1; OrderedLocusNames=Os07g0572800, LOC_Os07g38530;
GN ORFNames=OJ1121_A05.35-1, OJ1121_A05.35-2, OJ1699_E05.11-1,
GN OJ1699_E05.11-2, OsJ_023858;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0D598-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0D598-2; Sequence=VSP_035532, VSP_035533;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. WNK subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Was named WNK/'with no lysine(K)' because key residues for
CC catalysis, including the lysine involved in ATP binding, are either not
CC conserved or differ compared to the residues described in other kinase
CC family proteins. {ECO:0000250|UniProtKB:Q9H4A3}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC83145.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAC83145.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD31874.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD31875.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAZ40375.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AP003845; BAC83145.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP003845; BAC83146.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005908; BAD31874.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP005908; BAD31875.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008213; BAF21975.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT02251.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ40375.1; ALT_INIT; Genomic_DNA.
DR EMBL; AK100426; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015647040.1; XM_015791554.1. [Q0D598-1]
DR AlphaFoldDB; Q0D598; -.
DR SMR; Q0D598; -.
DR STRING; 4530.OS07T0572800-01; -.
DR iPTMnet; Q0D598; -.
DR PaxDb; Q0D598; -.
DR PRIDE; Q0D598; -.
DR EnsemblPlants; Os07t0572800-01; Os07t0572800-01; Os07g0572800. [Q0D598-1]
DR GeneID; 4343678; -.
DR Gramene; Os07t0572800-01; Os07t0572800-01; Os07g0572800. [Q0D598-1]
DR KEGG; osa:4343678; -.
DR eggNOG; KOG0584; Eukaryota.
DR HOGENOM; CLU_000288_142_2_1; -.
DR InParanoid; Q0D598; -.
DR OMA; HCEVDAQ; -.
DR OrthoDB; 695382at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR ExpressionAtlas; Q0D598; baseline and differential.
DR Genevisible; Q0D598; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..704
FT /note="Probable serine/threonine-protein kinase WNK1"
FT /id="PRO_0000351670"
FT DOMAIN 27..284
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 499..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 174
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9JIH7"
FT BINDING 107..110
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT BINDING 157
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT VAR_SEQ 649..654
FT /note="YHSRHP -> PKGPAL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_035532"
FT VAR_SEQ 655..704
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_035533"
FT CONFLICT 11
FT /note="A -> V (in Ref. 5; AK100426)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="E -> K (in Ref. 5; AK100426)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 704 AA; 79820 MW; 439F27F7699460FE CRC64;
MMGPKANAAA AGDLPEYAEV DPTGRYGRYN DVLGKGASKT VYRAFDEYQG MEVAWNQVKL
HDFLQSPEDL ERLYCEIHLL KTLKHRNIMK FYTSWVDVSR RNINFITEMF TSGTLRQYRQ
KHMRVNIWAV KHWCRQILSG LLYLHSHDPP IIHRDLKCDN IFVNGNQGEV KIGDLGLAAI
LRKSHAVHCV GTPEFMAPEV YEEEYNELVD IYSFGMCVLE MVTFEYPYSE CTHPVQIYKK
VISGTKPEAL YKVKDPMVRQ FVEKCLATAS RRLSARELLK DPFLQVDDLV FCPGDGDYSL
MNYLRQPYLE HAYSNVSMMS NGLSESIDED TPTEDRWDCE DDDIKADGID LFNGHEDEPL
GNVDITIKGR KSEDGSIFLR LRIADNDGHV RNIYFPFDIE ADTALSVATE MVAELDITDH
EVTRIAEMID GEVSALVPDW RPGPGIEESQ DTTYCHNCGS NVSSCGSLYA YMSSAARGCQ
CAELHGRFEE ITFQANGEQT DLQDSGGSSD DGGGQTQHVK DQEAVHSNGF VQMGRRGPRD
QFCFSSFQEQ SCSPRHYEYD TSLQAKGFDM KHEVKMAKYK ARKMAHLRRA IHPSLDFDNL
NGERRMKSSL NKLQSFHIGK NHNFRIPTCE RSPGARDAEE DPDIFNLAYH SRHPDPGAQR
ARHCEVDAQS SPDLMFTARS YYTGAQLPTN LPRTKSVTLN AVDA
