WNK2_ORYSJ
ID WNK2_ORYSJ Reviewed; 621 AA.
AC Q65X23; B9FM26; Q7XB94; Q94HK9;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Probable serine/threonine-protein kinase WNK2;
DE Short=OsWNK2;
DE EC=2.7.11.1;
DE AltName: Full=Protein DISEASE RELATIVE SIGNAL 1;
DE AltName: Full=Protein kinase with no lysine 2;
GN Name=WNK2; Synonyms=RDRS1; OrderedLocusNames=Os05g0108300, LOC_Os05g01780;
GN ORFNames=OsJ_16836 {ECO:0000312|EMBL:EEE62052.1}, OSJNBa0068N01.12;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Yao Q., Peng R., Xiong A.;
RT "Characterization of a protein kinase gene in rice.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q65X23-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q65X23-2; Sequence=VSP_035536, VSP_035537, VSP_035538;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. WNK subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Was named WNK/'with no lysine(K)' because key residues for
CC catalysis, including the lysine involved in ATP binding, are either not
CC conserved or differ compared to the residues described in other kinase
CC family proteins. {ECO:0000250|UniProtKB:Q9H4A3}.
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DR EMBL; AY336987; AAQ01193.1; -; mRNA.
DR EMBL; AC079022; AAK73135.1; -; Genomic_DNA.
DR EMBL; AC129716; AAU44135.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16344.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS91895.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE62052.1; -; Genomic_DNA.
DR EMBL; AK070061; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015640623.1; XM_015785137.1. [Q65X23-1]
DR AlphaFoldDB; Q65X23; -.
DR SMR; Q65X23; -.
DR STRING; 4530.OS05T0108300-01; -.
DR PaxDb; Q65X23; -.
DR PRIDE; Q65X23; -.
DR EnsemblPlants; Os05t0108300-01; Os05t0108300-01; Os05g0108300. [Q65X23-1]
DR GeneID; 4337579; -.
DR Gramene; Os05t0108300-01; Os05t0108300-01; Os05g0108300. [Q65X23-1]
DR KEGG; osa:4337579; -.
DR eggNOG; KOG0584; Eukaryota.
DR HOGENOM; CLU_000288_142_3_1; -.
DR InParanoid; Q65X23; -.
DR OMA; LATCKIK; -.
DR OrthoDB; 695382at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q65X23; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR024678; Kinase_OSR1/WNK_CCT.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF12202; OSR1_C; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..621
FT /note="Probable serine/threonine-protein kinase WNK2"
FT /id="PRO_0000351672"
FT DOMAIN 28..286
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 438..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 501..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 527..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 600..621
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..465
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 175
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9JIH7"
FT BINDING 108..111
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT BINDING 158
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT VAR_SEQ 1..42
FT /note="MPPTPPPELDLLDTEPEFAEVDPTARYGRYTEVLGKGAFKTV -> MHAASC
FT STRRSSARAPSRRCILLPTLDFWALTCDSVC (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_035536"
FT VAR_SEQ 305..359
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_035537"
FT VAR_SEQ 547..565
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_035538"
FT CONFLICT 298
FT /note="Q -> L (in Ref. 7; AK070061)"
FT /evidence="ECO:0000305"
FT CONFLICT 539
FT /note="D -> N (in Ref. 7; AK070061)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 621 AA; 70101 MW; 1D1DEC0A8BF4805E CRC64;
MPPTPPPELD LLDTEPEFAE VDPTARYGRY TEVLGKGAFK TVYKAFDQLE GLEVAWNQIK
VGDILRNNDD LERLRSEVRL LKTLKHKNII KFYNSWLDKK NNNINFITEV FTSGTLRQYR
IKHKKVDVRA LKKWSRQILS GLVYLHSHDP PVIHRDLKCD NIFVNGNQGE VKIGDLGLAT
ILDNARSAHS IIGTPEFMAP ELYDEEYNEL VDIYAFGMCL LELVTFEYPY CECSNAAQIY
KKVSDGEKPS SLAKIEDPEV RFFIEKCIAK ASQRLSAQEL LMDPFLRDDG EKIFYPLQSN
TKASDGAGSS NSSMGYKYDR DASSMAIREH TGSFAEEHPS DRYIHSTMDP QAAAGRIITV
ESQMKDLNTI FLKLRIADST GHAQNIHFPF DIEADTSISV ATEMVVQLDL TDQDVTAIAE
MIDAEIRAHI PDWALEESVE NQGDERAHSE TDSSEADDET SELRNEPNAT HNGFVQEHLP
SGHKYWSDSP RRNIEMSHSA VEPHIGGNMP NGILKKNDTD DTVSNLGTSV DLPNPSMIDR
KSGVASVSTS PQSFDDEHIE ADVSERLVNL LAQQQEELNV LRRKHKADIE VILKGVPEEH
REETLTRCRL KADERNRSDK P
