ID   WNK3_ORYSJ              Reviewed;         601 AA.
AC   Q0D847; B7ER38; Q8H4Y8;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Probable serine/threonine-protein kinase WNK3;
DE            Short=OsWNK3;
DE            EC=2.7.11.1;
DE   AltName: Full=Protein kinase with no lysine 3;
GN   Name=WNK3; OrderedLocusNames=Os07g0185000, LOC_Os07g08750;
GN   ORFNames=OJ1046_F10.129-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1;
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC       kinase family. WNK subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC   -!- CAUTION: Was named WNK/'with no lysine(K)' because key residues for
CC       catalysis, including the lysine involved in ATP binding, are either not
CC       conserved or differ compared to the residues described in other kinase
CC       family proteins. {ECO:0000250|UniProtKB:Q9H4A3}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC24889.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP003861; BAC24889.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP008213; BAF20976.1; -; Genomic_DNA.
DR   EMBL; AP014963; BAT00359.1; -; Genomic_DNA.
DR   EMBL; AK100930; BAG94835.1; -; mRNA.
DR   RefSeq; XP_015646740.1; XM_015791254.1.
DR   AlphaFoldDB; Q0D847; -.
DR   SMR; Q0D847; -.
DR   STRING; 4530.OS07T0185000-01; -.
DR   PaxDb; Q0D847; -.
DR   PRIDE; Q0D847; -.
DR   EnsemblPlants; Os07t0185000-01; Os07t0185000-01; Os07g0185000.
DR   GeneID; 4342585; -.
DR   Gramene; Os07t0185000-01; Os07t0185000-01; Os07g0185000.
DR   KEGG; osa:4342585; -.
DR   eggNOG; KOG0584; Eukaryota.
DR   HOGENOM; CLU_000288_142_2_1; -.
DR   InParanoid; Q0D847; -.
DR   OMA; CKNCRPA; -.
DR   OrthoDB; 695382at2759; -.
DR   Proteomes; UP000000763; Chromosome 7.
DR   Proteomes; UP000059680; Chromosome 7.
DR   ExpressionAtlas; Q0D847; baseline and differential.
DR   Genevisible; Q0D847; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR   GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR   GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW   Serine/threonine-protein kinase; Transferase.
FT   CHAIN           1..601
FT                   /note="Probable serine/threonine-protein kinase WNK3"
FT                   /id="PRO_0000351673"
FT   DOMAIN          34..291
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          470..498
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          551..601
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        181
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9JIH7"
FT   BINDING         114..117
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT   BINDING         164
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H4A3"
SQ   SEQUENCE   601 AA;  65438 MW;  AE3495128FF6A8E6 CRC64;
     MMGALQQQSN GHGHGVLLLA EAGYAEVDPT GRYGRFNEIL GKGSSKIVYR GFDEWRGVEV
     AWNQVRLRDV VRGGGELERF YGEVHLLAAL RHRGIVRLHA YWVDAPRRAL NFVTELFVSG
     TLRQYRERHR RVSAAAVRRW CAQILDGLAY LHAHSPPIIH RDLKCDNIFV NGNQGEVKIG
     DLGLAAFRRG GGHARCVGTP EFMAPEVYDE SYDELADVYS FGMCVLEMVT LDYPYSECSN
     PIQIYKRVIS GIKPAALYRV SDPVVRQFIE RCLAPAARRP AARELLDDPF LLPLEDDGFF
     SGDGGDGHGG FGVGYYNLMY NYLHQPACID DHHACSNGGL SPSNSVGDND VDAAVQRGDD
     DGDNWLRDIH MLFDEDDDDA AAADANERVG GVDITIKGRR TDDGGVYLGL RIADKNGTGR
     GRIICFRFDT EADTAMTVAA EMVAELDITD HEVTRIAQLI DGKVAALVPG WRPGPATDDD
     DDDDLVGGGD DPDAPGGAAA ACCKNCRPAA SSSSSCGSLV DFMSSAAAAE RHGCRRCAEL
     HGRFEEITFQ ADDDEEEQHL QGSSSDTGGS NHEQHAMGKD KEVMNINGIA QDGTVQGSEQ
     P