WNK4_ORYSJ
ID WNK4_ORYSJ Reviewed; 612 AA.
AC Q6EU49; A0A0N7KFV2; A3AA15;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable serine/threonine-protein kinase WNK4;
DE Short=OsWNK4;
DE EC=2.7.11.1;
DE AltName: Full=Protein kinase with no lysine 4;
GN Name=WNK4; OrderedLocusNames=Os02g0672800, LOC_Os02g45130;
GN ORFNames=OJ1197_E09.9, OsJ_007637;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. WNK subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Was named WNK/'with no lysine(K)' because key residues for
CC catalysis, including the lysine involved in ATP binding, are either not
CC conserved or differ compared to the residues described in other kinase
CC family proteins. {ECO:0000250|UniProtKB:Q9H4A3}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAZ24154.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004160; BAD27820.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09623.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80249.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ24154.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK073845; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015625440.1; XM_015769954.1.
DR AlphaFoldDB; Q6EU49; -.
DR SMR; Q6EU49; -.
DR STRING; 4530.OS02T0672800-01; -.
DR PaxDb; Q6EU49; -.
DR PRIDE; Q6EU49; -.
DR EnsemblPlants; Os02t0672800-01; Os02t0672800-01; Os02g0672800.
DR GeneID; 4330286; -.
DR Gramene; Os02t0672800-01; Os02t0672800-01; Os02g0672800.
DR KEGG; osa:4330286; -.
DR eggNOG; KOG0584; Eukaryota.
DR HOGENOM; CLU_000288_142_0_1; -.
DR InParanoid; Q6EU49; -.
DR OMA; WIDELNK; -.
DR OrthoDB; 695382at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6EU49; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR024678; Kinase_OSR1/WNK_CCT.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF12202; OSR1_C; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..612
FT /note="Probable serine/threonine-protein kinase WNK4"
FT /id="PRO_0000351674"
FT DOMAIN 25..282
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 172
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9JIH7"
FT BINDING 105..108
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT BINDING 155
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT CONFLICT 258
FT /note="Q -> R (in Ref. 5; AK073845)"
FT /evidence="ECO:0000305"
FT CONFLICT 599
FT /note="E -> G (in Ref. 5; AK073845)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 612 AA; 68710 MW; FB302C6247A94409 CRC64;
MEVFGDLDKV DDAECVEVDP TRRYIRYNEV LGRGAMKTVY KAFDEVEGIE VAWSQVEIDE
VMQSPDNLER LYSEVHLLKS LKHENVMKFY NYWVDDQKKT INVITELFTS GSLRQYRQKH
PRVDLKAIKN WARQVLRGLD YLHTHQPPII HRDLKCDNIF VNGNHGEVKI GDLGLATVML
TPRAKSVIGT PEFMAPELYD ENYDELVDIY SFGMCMLEMF TLEYPYSECT NAAQIFKKVS
KGVKPAALAK ITNIQAKQFI DKCLVPASER LSAKELLQDP FLCSDNSSVL VGTKFPSSLP
KSVDVSLEAL HMDVDTNESM CTSTCKRNDL GGPHRSVLEF TRTNKNTELK LTGEKLDDNS
VSLVLRIADL CGHARNIHFL FYLDSDTAMS VAAEMVEQLE LADCDVTFIA DFIDLLIVNL
VPGQQLMNDA VMSTSSESKM GESEHVITSQ QHPSELTHDY VLVEGMMHSK EANASPSDYI
DSLLNATNLG GPNSSEGSDI SVQLDGSSKS LSEYGVDEYR TLECGAYKGT DKLGCRHPLS
NGSSNFAIFQ MDQASHHSEL VIGASVSITE NRDVLNGELG LIEAQYEQWF RELTRMREEA
LEGARKKWLP DK
