WNK6_ARATH
ID WNK6_ARATH Reviewed; 567 AA.
AC Q8S8Y8; Q2V3U7; Q9LSA2;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Probable serine/threonine-protein kinase WNK6;
DE Short=AtWNK6;
DE EC=2.7.11.1;
DE AltName: Full=Protein kinase with no lysine 6;
GN Name=WNK6; OrderedLocusNames=At3g18750; ORFNames=MVE11.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=12506983; DOI=10.1271/bbb.66.2429;
RA Nakamichi N., Murakami-Kojima M., Sato E., Kishi Y., Yamashino T.,
RA Mizuno T.;
RT "Compilation and characterization of a novel WNK family of protein kinases
RT in Arabiodpsis thaliana with reference to circadian rhythms.";
RL Biosci. Biotechnol. Biochem. 66:2429-2436(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May regulate flowering time by modulating the photoperiod
CC pathway. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8S8Y8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8S8Y8-2; Sequence=VSP_035530;
CC -!- INDUCTION: Expressed with a circadian rhythm showing a peak before
CC dawn. {ECO:0000269|PubMed:12506983}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. WNK subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- CAUTION: Was named WNK/'with no lysine(K)' because key residues for
CC catalysis, including the lysine involved in ATP binding, are either not
CC conserved or differ compared to the residues described in other kinase
CC family proteins. {ECO:0000250|UniProtKB:Q9H4A3}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01801.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB084270; BAB91129.1; -; mRNA.
DR EMBL; AB026654; BAB01801.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76139.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76140.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76141.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64345.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64346.1; -; Genomic_DNA.
DR EMBL; AK175997; BAD43760.1; -; mRNA.
DR EMBL; BT030341; ABO38754.1; -; mRNA.
DR RefSeq; NP_001030723.1; NM_001035646.2. [Q8S8Y8-2]
DR RefSeq; NP_001189928.1; NM_001202999.2. [Q8S8Y8-1]
DR RefSeq; NP_001319584.1; NM_001338354.1. [Q8S8Y8-2]
DR RefSeq; NP_001326380.1; NM_001338356.1. [Q8S8Y8-1]
DR RefSeq; NP_188505.1; NM_112761.3. [Q8S8Y8-1]
DR AlphaFoldDB; Q8S8Y8; -.
DR SMR; Q8S8Y8; -.
DR STRING; 3702.AT3G18750.3; -.
DR PaxDb; Q8S8Y8; -.
DR PRIDE; Q8S8Y8; -.
DR ProteomicsDB; 243084; -. [Q8S8Y8-1]
DR EnsemblPlants; AT3G18750.1; AT3G18750.1; AT3G18750. [Q8S8Y8-1]
DR EnsemblPlants; AT3G18750.2; AT3G18750.2; AT3G18750. [Q8S8Y8-2]
DR EnsemblPlants; AT3G18750.3; AT3G18750.3; AT3G18750. [Q8S8Y8-1]
DR EnsemblPlants; AT3G18750.5; AT3G18750.5; AT3G18750. [Q8S8Y8-1]
DR EnsemblPlants; AT3G18750.6; AT3G18750.6; AT3G18750. [Q8S8Y8-2]
DR GeneID; 821406; -.
DR Gramene; AT3G18750.1; AT3G18750.1; AT3G18750. [Q8S8Y8-1]
DR Gramene; AT3G18750.2; AT3G18750.2; AT3G18750. [Q8S8Y8-2]
DR Gramene; AT3G18750.3; AT3G18750.3; AT3G18750. [Q8S8Y8-1]
DR Gramene; AT3G18750.5; AT3G18750.5; AT3G18750. [Q8S8Y8-1]
DR Gramene; AT3G18750.6; AT3G18750.6; AT3G18750. [Q8S8Y8-2]
DR KEGG; ath:AT3G18750; -.
DR Araport; AT3G18750; -.
DR TAIR; locus:2093994; AT3G18750.
DR eggNOG; KOG0584; Eukaryota.
DR HOGENOM; CLU_000288_142_0_1; -.
DR InParanoid; Q8S8Y8; -.
DR OMA; FINGHRG; -.
DR OrthoDB; 695382at2759; -.
DR PhylomeDB; Q8S8Y8; -.
DR PRO; PR:Q8S8Y8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8S8Y8; baseline and differential.
DR Genevisible; Q8S8Y8; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; TAS:TAIR.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Coiled coil; Kinase; Nucleotide-binding;
KW Reference proteome; Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..567
FT /note="Probable serine/threonine-protein kinase WNK6"
FT /id="PRO_0000351664"
FT DOMAIN 28..285
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 499..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 519..553
FT /evidence="ECO:0000255"
FT COMPBIAS 499..513
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 175
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9JIH7"
FT BINDING 108..111
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT BINDING 158
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q9H4A3"
FT VAR_SEQ 192..258
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_035530"
SQ SEQUENCE 567 AA; 64866 MW; 9D0AB562D4E94BB2 CRC64;
MEGTDDASAL QEPPDPEVLE VDPTFRYIRY KEVIGKGAFK TVYKAFDEVD GIEVAWNQVR
IDDVLQSPNC LERLYSEVRL LKSLKHNNII RFYNSWIDDK NKTVNIITEL FTSGSLRHYR
KKHRKVNMKA VKNWARQILM GLRYLHGQEP PIIHRDLKCD NIFINGNHGE VKIGDLGLAT
VMEQANAKSV IGTPEFMAPE LYDENYNELA DIYSFGMCML EMVTFDYPYC ECKNSAQIYK
KVSSGIKPAS LSRVKDPEVK QFIEKCLLPA SERLSAKELL LDPFLQLNGL TMNNPLPLPD
IVMPKEGAFG DRCLMSEGPP TTRPSKTLSI DLDEDSNLPI VTFSDNSGSR CIEVRRAKRG
NFFVLKGEEN DEQSVSLILR IVDENGRVRN IHFLFYQEGD TASKVSSEMV EQLELTDQNV
TFIAELIDIL LVNMIPTWKT DVTVDHLIHS QLNQNSRSHH NEAKPQKQEE TVFHDTCELV
SHSCNSDCPR SDEEDKQCVD ATKGEDKSSI QEVEEATEPV SLEEEERLRQ ELEEIEAKYQ
EDMKEIATKR EEAIMETKKK LSLMKLK
