WNT4A_DANRE
ID WNT4A_DANRE Reviewed; 352 AA.
AC P47793; Q92049;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Protein Wnt-4a;
DE Flags: Precursor;
GN Name=wnt4a; Synonyms=wnt-4, wnt4;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Embryo;
RX PubMed=8541205; DOI=10.1016/0925-4773(95)00386-f;
RA Ungar A.R., Kelly G.M., Moon R.T.;
RT "Wnt4 affects morphogenesis when misexpressed in the zebrafish embryo.";
RL Mech. Dev. 52:153-164(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RA Blader P., Straehle U., Ingham P.W.;
RT "Three Wnt genes expressed in a wide variety of tissues during development
RT of the zebrafish, Danio rerio: developmental and evolutionary
RT perspectives.";
RL Dev. Genes Evol. 206:3-13(1996).
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors (Probable). Plays an important role in
CC embryonic development (PubMed:8541205). {ECO:0000269|PubMed:8541205,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- TISSUE SPECIFICITY: Caudal forebrain and neural keel, the floor plate,
CC the gill slit and the developing pronephros.
CC -!- DEVELOPMENTAL STAGE: First detected at the end of gastrulation in the
CC anterior neuroectoderm. Expression increases in the developing brain
CC during somitogenesis but has gone by 36 hours. Expression in the floor
CC plate begins at the 10 somite stage and persists at 72 hours.
CC {ECO:0000269|Ref.2}.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC inhibition. {ECO:0000250|UniProtKB:P27467,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U25141; AAA96004.1; -; mRNA.
DR EMBL; U51267; AAA96518.1; -; mRNA.
DR RefSeq; NP_001035477.1; NM_001040387.1.
DR AlphaFoldDB; P47793; -.
DR SMR; P47793; -.
DR STRING; 7955.ENSDARP00000095904; -.
DR PaxDb; P47793; -.
DR Ensembl; ENSDART00000105133; ENSDARP00000095904; ENSDARG00000071208.
DR GeneID; 30123; -.
DR KEGG; dre:30123; -.
DR CTD; 54361; -.
DR ZFIN; ZDB-GENE-980526-352; wnt4.
DR eggNOG; KOG3913; Eukaryota.
DR GeneTree; ENSGT00940000159654; -.
DR HOGENOM; CLU_033039_1_0_1; -.
DR InParanoid; P47793; -.
DR OMA; CWRAMPP; -.
DR OrthoDB; 745245at2759; -.
DR PhylomeDB; P47793; -.
DR TreeFam; TF105310; -.
DR Reactome; R-DRE-3238698; WNT ligand biogenesis and trafficking.
DR Reactome; R-DRE-4086400; PCP/CE pathway.
DR PRO; PR:P47793; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 11.
DR Bgee; ENSDARG00000071208; Expressed in neural tube and 43 other tissues.
DR ExpressionAtlas; P47793; baseline.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:UniProtKB.
DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; IMP:ZFIN.
DR GO; GO:0021986; P:habenula development; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IGI:ZFIN.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IPI:ZFIN.
DR GO; GO:0048841; P:regulation of axon extension involved in axon guidance; IMP:ZFIN.
DR GO; GO:0070654; P:sensory epithelium regeneration; IEP:ZFIN.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR009142; Wnt4.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01844; WNT4PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..352
FT /note="Protein Wnt-4a"
FT /id="PRO_0000041424"
FT LIPID 212
FT /note="O-palmitoleoyl serine; by PORCN"
FT /evidence="ECO:0000250|UniProtKB:P56704"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 298
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 78..89
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 128..136
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 138..155
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 206..220
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 208..215
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 280..312
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 297..307
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 311..351
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 327..342
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 329..339
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 334..335
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT CONFLICT 245..246
FT /note="EL -> DV (in Ref. 2; AAA96518)"
FT /evidence="ECO:0000305"
FT CONFLICT 335
FT /note="C -> S (in Ref. 2; AAA96518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 352 AA; 39431 MW; D63CBF9F00886633 CRC64;
MSSEYLIRSL LMLFLALFSA NASNWLYLAK LSSVGSISDE ETCEKLRGLI QRQVQICKRN
VEVMDAVRRG AQLAIDECQY QFRNRRWNCS TLESVPVFGK VVTQGTREAA FVYAISAASV
AFAVTRACSS GELDKCGCDR NVHGVSPEGF QWSGCSDNIA YGVAFSQSFV DIRERSKGQS
SNRALMNLHN NEAGRKAILN HMRVECKCHG VSGSCEVKTC WKAMPPFRKV GNVIKEKFDG
ATEVELRKVG TTKVLVPRNS QFKPHTDEDL VYLDPSPDFC EHDPRTPGIM GTAGRFCNKT
SKAIDGCELM CCGRGFHTEE VEVVDRCSCK FHWCCYVKCK QCRKMVEMHT CR
