WNT4_RAT
ID WNT4_RAT Reviewed; 351 AA.
AC Q9QXQ5;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Protein Wnt-4;
DE Flags: Precursor;
GN Name=Wnt4; Synonyms=Wnt-4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Corpus luteum;
RA Lacher M.D., Walther P.R., Lareu R., Dharmarajan A.M., Friis R.R.;
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors. Plays an important role in the embryonic
CC development of the urogenital tract and the lung. Required for normal
CC mesenchyme to epithelium transition during embryonic kidney
CC development. Required for the formation of early epithelial renal
CC vesicles during kidney development. Required for normal formation of
CC the Mullerian duct in females, and normal levels of oocytes in the
CC ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid
CC dehydrogenase in the ovary. Required for normal lung development and
CC for normal patterning of trachael cartilage rings.
CC {ECO:0000250|UniProtKB:P22724}.
CC -!- SUBUNIT: Interacts with PORCN (By similarity). Interacts with PKD1 (By
CC similarity). {ECO:0000250|UniProtKB:P22724,
CC ECO:0000250|UniProtKB:P56705}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC inhibition. {ECO:0000250|UniProtKB:P27467,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR EMBL; AF188608; AAF15589.1; -; mRNA.
DR RefSeq; NP_445854.1; NM_053402.2.
DR AlphaFoldDB; Q9QXQ5; -.
DR SMR; Q9QXQ5; -.
DR STRING; 10116.ENSRNOP00000018064; -.
DR GlyGen; Q9QXQ5; 2 sites.
DR PaxDb; Q9QXQ5; -.
DR GeneID; 84426; -.
DR KEGG; rno:84426; -.
DR UCSC; RGD:621348; rat.
DR CTD; 54361; -.
DR RGD; 621348; Wnt4.
DR eggNOG; KOG3913; Eukaryota.
DR InParanoid; Q9QXQ5; -.
DR OrthoDB; 745245at2759; -.
DR PhylomeDB; Q9QXQ5; -.
DR Reactome; R-RNO-3238698; WNT ligand biogenesis and trafficking.
DR Reactome; R-RNO-4086400; PCP/CE pathway.
DR PRO; PR:Q9QXQ5; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0009986; C:cell surface; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0031012; C:extracellular matrix; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:RGD.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; ISO:RGD.
DR GO; GO:0048018; F:receptor ligand activity; ISO:RGD.
DR GO; GO:0003714; F:transcription corepressor activity; ISO:RGD.
DR GO; GO:0030325; P:adrenal gland development; ISO:RGD.
DR GO; GO:0048856; P:anatomical structure development; ISO:RGD.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; ISO:RGD.
DR GO; GO:0048754; P:branching morphogenesis of an epithelial tube; ISO:RGD.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0045165; P:cell fate commitment; ISO:RGD.
DR GO; GO:0071392; P:cellular response to estradiol stimulus; IEP:RGD.
DR GO; GO:0009267; P:cellular response to starvation; ISO:RGD.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; ISO:RGD.
DR GO; GO:0001838; P:embryonic epithelial tube formation; ISO:RGD.
DR GO; GO:0001837; P:epithelial to mesenchymal transition; ISO:RGD.
DR GO; GO:0008585; P:female gonad development; ISO:RGD.
DR GO; GO:0030237; P:female sex determination; ISO:RGD.
DR GO; GO:0007276; P:gamete generation; ISO:RGD.
DR GO; GO:0042445; P:hormone metabolic process; ISO:RGD.
DR GO; GO:0033080; P:immature T cell proliferation in thymus; ISO:RGD.
DR GO; GO:0001822; P:kidney development; ISO:RGD.
DR GO; GO:0060993; P:kidney morphogenesis; ISO:RGD.
DR GO; GO:0001889; P:liver development; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; ISO:RGD.
DR GO; GO:0061180; P:mammary gland epithelium development; ISO:RGD.
DR GO; GO:0060231; P:mesenchymal to epithelial transition; ISO:RGD.
DR GO; GO:0072164; P:mesonephric tubule development; ISO:RGD.
DR GO; GO:0001823; P:mesonephros development; ISO:RGD.
DR GO; GO:0072210; P:metanephric nephron development; ISO:RGD.
DR GO; GO:0072273; P:metanephric nephron morphogenesis; ISO:RGD.
DR GO; GO:0072174; P:metanephric tubule formation; ISO:RGD.
DR GO; GO:0001656; P:metanephros development; ISO:RGD.
DR GO; GO:2000180; P:negative regulation of androgen biosynthetic process; ISO:RGD.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0045596; P:negative regulation of cell differentiation; ISO:RGD.
DR GO; GO:0030336; P:negative regulation of cell migration; ISO:RGD.
DR GO; GO:0040037; P:negative regulation of fibroblast growth factor receptor signaling pathway; ISO:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:2000019; P:negative regulation of male gonad development; ISO:RGD.
DR GO; GO:0010894; P:negative regulation of steroid biosynthetic process; ISO:RGD.
DR GO; GO:0061369; P:negative regulation of testicular blood vessel morphogenesis; ISO:RGD.
DR GO; GO:2000225; P:negative regulation of testosterone biosynthetic process; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0061045; P:negative regulation of wound healing; ISO:RGD.
DR GO; GO:0072006; P:nephron development; ISO:RGD.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0035567; P:non-canonical Wnt signaling pathway; ISO:RGD.
DR GO; GO:0038030; P:non-canonical Wnt signaling pathway via MAPK cascade; ISO:RGD.
DR GO; GO:0048599; P:oocyte development; ISO:RGD.
DR GO; GO:0061205; P:paramesonephric duct development; ISO:RGD.
DR GO; GO:0032349; P:positive regulation of aldosterone biosynthetic process; ISO:RGD.
DR GO; GO:0030501; P:positive regulation of bone mineralization; ISO:RGD.
DR GO; GO:0032967; P:positive regulation of collagen biosynthetic process; ISO:RGD.
DR GO; GO:2000066; P:positive regulation of cortisol biosynthetic process; ISO:RGD.
DR GO; GO:0061184; P:positive regulation of dermatome development; ISO:RGD.
DR GO; GO:0051894; P:positive regulation of focal adhesion assembly; ISO:RGD.
DR GO; GO:0043547; P:positive regulation of GTPase activity; ISO:RGD.
DR GO; GO:0045836; P:positive regulation of meiotic nuclear division; ISO:RGD.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; ISO:RGD.
DR GO; GO:0051496; P:positive regulation of stress fiber assembly; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0022407; P:regulation of cell-cell adhesion; ISO:RGD.
DR GO; GO:0072033; P:renal vesicle formation; ISO:RGD.
DR GO; GO:0072034; P:renal vesicle induction; ISO:RGD.
DR GO; GO:0032355; P:response to estradiol; IEP:RGD.
DR GO; GO:0007548; P:sex differentiation; ISO:RGD.
DR GO; GO:0051145; P:smooth muscle cell differentiation; ISO:RGD.
DR GO; GO:0060126; P:somatotropin secreting cell differentiation; ISO:RGD.
DR GO; GO:0033077; P:T cell differentiation in thymus; ISO:RGD.
DR GO; GO:0060748; P:tertiary branching involved in mammary gland duct morphogenesis; ISO:RGD.
DR GO; GO:0060129; P:thyroid-stimulating hormone-secreting cell differentiation; ISO:RGD.
DR GO; GO:0035239; P:tube morphogenesis; ISO:RGD.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR009142; Wnt4.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01844; WNT4PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..351
FT /note="Protein Wnt-4"
FT /id="PRO_0000041423"
FT LIPID 212
FT /note="O-palmitoleoyl serine; by PORCN"
FT /evidence="ECO:0000250|UniProtKB:P56704"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 297
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 78..89
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 128..136
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 138..155
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 206..220
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 208..215
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 280..311
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 296..306
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 310..350
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 326..341
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 328..338
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 333..334
FT /evidence="ECO:0000250|UniProtKB:P28026"
SQ SEQUENCE 351 AA; 39044 MW; 5F8D80C3B4502BA1 CRC64;
MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI QRQVQMCKRN
LEVMDSVRHG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK VVTQGTREAA FVYAISSAGV
AFAVTRACSS GDLEKCGCDR TVHGVSPQGF QWSGCSDNIA YGVAFSQSFV DVRERSKGAS
SSRALMNLHN NEAGRKAILT HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG
ATEVEPRRVG SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDMRSGVLG TRGRTCNKTS
KAIDGCELLC CGRGFHTAHV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC R
