CAN3_ORYSJ
ID CAN3_ORYSJ Reviewed; 377 AA.
AC Q2QYR1; C7JA21;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 03-SEP-2014, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Probable staphylococcal-like nuclease CAN3;
DE EC=3.1.31.-;
DE AltName: Full=Calcium-dependent nuclease 3;
DE Short=Ca(2+)-dependent nuclease 3;
GN OrderedLocusNames=Os12g0109200, LOC_Os12g01830; ORFNames=OSJNBa0024J08;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Enzyme that catalyzes the hydrolysis of both DNA and RNA at
CC the 5' position of the phosphodiester bond. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC ProRule:PRU00272}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABA96197.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAG96050.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=BAH95483.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX000492; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DP000011; ABA96197.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008218; BAH95483.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014968; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK103380; BAG96050.1; ALT_SEQ; mRNA.
DR RefSeq; XP_015618302.1; XM_015762816.1.
DR AlphaFoldDB; Q2QYR1; -.
DR SMR; Q2QYR1; -.
DR PaxDb; Q2QYR1; -.
DR PRIDE; Q2QYR1; -.
DR GeneID; 4351283; -.
DR KEGG; osa:4351283; -.
DR OrthoDB; 1333771at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.90; -; 1.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR Pfam; PF00565; SNase; 1.
DR SMART; SM00318; SNc; 1.
DR SUPFAM; SSF50199; SSF50199; 1.
DR PROSITE; PS50830; TNASE_3; 1.
PE 2: Evidence at transcript level;
KW Calcium; Cell membrane; Endonuclease; Hydrolase; Lipoprotein; Membrane;
KW Metal-binding; Myristate; Nuclease; Palmitate; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..377
FT /note="Probable staphylococcal-like nuclease CAN3"
FT /id="PRO_0000430202"
FT DOMAIN 177..353
FT /note="TNase-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT REGION 15..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..51
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 260
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT ACT_SITE 268
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT ACT_SITE 302
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT BINDING 190
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT BINDING 265
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT LIPID 7
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 377 AA; 42474 MW; 664CE968CA9CB030 CRC64;
MGNILRCFKG DDDGGDHYPY YKPTSRPHYQ PPHYHGQPAA PPAPPQQQPL GPHGVTPSTV
GVAALAHDLL NFESTSMVPD GLSQHVVSSR KAQVKWYQKL LEAYKNTTPP PKTPADAAQL
IARALNMIQR ADLEITSNYV SPQGILEFYN FPIPSLPSAS SNYQPSSLPE GVQFVLNTLP
VYDKCIGDGD GFTAYVSTTD PRESANVPLE VHELVIARTQ ARKCRDYQSA DALLSSLDEA
GYKIISCSDD EVLARKYRIR MRGIDAPELK MPYGRESRNA LVKLIGGKSV KIYVYDLDQF
GRYVGDIYCN NLFIQEQMLK NGHAWHFKTY DKRPEFARWE REAKAANRGL WASGNPEKPW
DWRRDQRNAR QDAIQVY
