WNT6_MOUSE
ID WNT6_MOUSE Reviewed; 364 AA.
AC P22727; Q80ZM9;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Protein Wnt-6;
DE Flags: Precursor;
GN Name=Wnt6; Synonyms=Wnt-6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2279700; DOI=10.1101/gad.4.12b.2319;
RA Gavin B.J., McMahon J.A., McMahon A.P.;
RT "Expression of multiple novel Wnt-1/int-1-related genes during fetal and
RT adult mouse development.";
RL Genes Dev. 4:2319-2332(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Limb;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH PORCN.
RX PubMed=10866835; DOI=10.1046/j.1432-1033.2000.01478.x;
RA Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.;
RT "The evolutionarily conserved porcupine gene family is involved in the
RT processing of the Wnt family.";
RL Eur. J. Biochem. 267:4300-4311(2000).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=22370641; DOI=10.1038/onc.2012.40;
RA Yuan G., Regel I., Lian F., Friedrich T., Hitkova I., Hofheinz R.D.,
RA Stroebel P., Langer R., Keller G., Roecken C., Zimmermann W., Schmid R.M.,
RA Ebert M.P.A., Burgermeister E.;
RT "WNT6 is a novel target gene of caveolin-1 promoting chemoresistance to
RT epirubicin in human gastric cancer cells.";
RL Oncogene 32:375-387(2013).
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors. Probable developmental protein. May be a
CC signaling molecule which affects the development of discrete regions of
CC tissues. Is likely to signal over only few cell diameters.
CC -!- SUBUNIT: Interacts with PORCN. {ECO:0000269|PubMed:10866835}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- TISSUE SPECIFICITY: Detected in ileum, colon and stomach (at protein
CC level). {ECO:0000269|PubMed:22370641}.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC inhibition. {ECO:0000250|UniProtKB:P27467,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR EMBL; M89800; AAA40569.1; -; mRNA.
DR EMBL; AK133954; BAE21949.1; -; mRNA.
DR EMBL; CH466548; EDL00351.1; -; Genomic_DNA.
DR EMBL; BC048700; AAH48700.1; -; mRNA.
DR CCDS; CCDS15056.1; -.
DR PIR; F36470; F36470.
DR RefSeq; NP_033552.2; NM_009526.3.
DR RefSeq; XP_006495950.1; XM_006495887.3.
DR RefSeq; XP_006495951.1; XM_006495888.3.
DR AlphaFoldDB; P22727; -.
DR SMR; P22727; -.
DR BioGRID; 204579; 1.
DR IntAct; P22727; 1.
DR STRING; 10090.ENSMUSP00000006716; -.
DR GlyGen; P22727; 2 sites.
DR PhosphoSitePlus; P22727; -.
DR MaxQB; P22727; -.
DR PaxDb; P22727; -.
DR PRIDE; P22727; -.
DR ProteomicsDB; 299774; -.
DR Antibodypedia; 34291; 249 antibodies from 34 providers.
DR DNASU; 22420; -.
DR Ensembl; ENSMUST00000006716; ENSMUSP00000006716; ENSMUSG00000033227.
DR GeneID; 22420; -.
DR KEGG; mmu:22420; -.
DR UCSC; uc007bne.1; mouse.
DR CTD; 7475; -.
DR MGI; MGI:98960; Wnt6.
DR VEuPathDB; HostDB:ENSMUSG00000033227; -.
DR eggNOG; KOG3913; Eukaryota.
DR GeneTree; ENSGT00940000159281; -.
DR HOGENOM; CLU_033039_1_3_1; -.
DR InParanoid; P22727; -.
DR OMA; EQTNCKC; -.
DR OrthoDB; 624528at2759; -.
DR PhylomeDB; P22727; -.
DR TreeFam; TF105310; -.
DR Reactome; R-MMU-3238698; WNT ligand biogenesis and trafficking.
DR BioGRID-ORCS; 22420; 2 hits in 75 CRISPR screens.
DR PRO; PR:P22727; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P22727; protein.
DR Bgee; ENSMUSG00000033227; Expressed in lumbar dorsal root ganglion and 186 other tissues.
DR Genevisible; P22727; MM.
DR GO; GO:0009986; C:cell surface; IDA:BHF-UCL.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0031012; C:extracellular matrix; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR GO; GO:0005102; F:signaling receptor binding; TAS:MGI.
DR GO; GO:0009887; P:animal organ morphogenesis; TAS:MGI.
DR GO; GO:0009798; P:axis specification; IDA:MGI.
DR GO; GO:0001658; P:branching involved in ureteric bud morphogenesis; IGI:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; TAS:MGI.
DR GO; GO:0060684; P:epithelial-mesenchymal cell signaling; IDA:MGI.
DR GO; GO:0072080; P:nephron tubule development; IDA:MGI.
DR GO; GO:0072079; P:nephron tubule formation; IDA:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:MGI.
DR GO; GO:0070172; P:positive regulation of tooth mineralization; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0007165; P:signal transduction; TAS:MGI.
DR GO; GO:0016055; P:Wnt signaling pathway; IDA:MGI.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR009143; Wnt6.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01845; WNT6PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..364
FT /note="Protein Wnt-6"
FT /id="PRO_0000041441"
FT REGION 140..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 227
FT /note="O-palmitoleoyl serine; by PORCN"
FT /evidence="ECO:0000250|UniProtKB:P56704"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 310
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 75..86
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 123..131
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 133..171
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 221..235
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 223..230
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 293..324
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 309..319
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 323..363
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 339..354
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 341..351
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 346..347
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT CONFLICT 233
FT /note="R -> S (in Ref. 1; AAA40569)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 364 AA; 39656 MW; 6F28CE191F98A0AC CRC64;
MLPPVPSRLG LLLLLLCPAH VDGLWWAVGS PLVMDPTSIC RKARRLAGRQ AELCQAEPEV
VAELARGARL GVRECQFQFR FRRWNCSSHS KAFGRVLQQD IRETAFVFAI TAAGASHAVT
QACSMGELLQ CGCQAPRGRA PPRPSGLLGT PGPPGPTGSP DASAAWEWGG CGDDVDFGDE
KSRLFMDAQH KRGRGDIRAL VQLHNNEAGR LAVRSHTRTE CKCHGLSGSC ALRTCWQKLP
PFREVGARLL ERFHGASRVM GTNDGKALLP AVRTLKPPGR ADLLYAADSP DFCAPNRRTG
SPGTRGRACN SSAPDLSGCD LLCCGRGHRQ ESVQLEENCL CRFHWCCVVQ CHRCRVRKEL
SLCL
