CAN4_ORYSJ
ID CAN4_ORYSJ Reviewed; 495 AA.
AC Q2QUS0; A0A0P0Y8P5;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable staphylococcal-like nuclease CAN4;
DE EC=3.1.31.-;
DE AltName: Full=Calcium-dependent nuclease 4;
DE Short=Ca(2+)-dependent nuclease 4;
GN OrderedLocusNames=Os12g0255200, LOC_Os12g15314; ORFNames=OSJNBa0036A15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Enzyme that catalyzes the hydrolysis of both DNA and RNA at
CC the 5' position of the phosphodiester bond. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC ProRule:PRU00272}.
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DR EMBL; AL928776; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DP000011; ABA96961.2; -; Genomic_DNA.
DR EMBL; AP008218; BAH95603.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT16555.1; -; Genomic_DNA.
DR EMBL; AK068445; BAG90912.1; -; mRNA.
DR RefSeq; XP_015620732.1; XM_015765246.1.
DR AlphaFoldDB; Q2QUS0; -.
DR SMR; Q2QUS0; -.
DR STRING; 4530.OS12T0255200-01; -.
DR PaxDb; Q2QUS0; -.
DR PRIDE; Q2QUS0; -.
DR EnsemblPlants; Os12t0255200-01; Os12t0255200-01; Os12g0255200.
DR GeneID; 9272184; -.
DR Gramene; Os12t0255200-01; Os12t0255200-01; Os12g0255200.
DR KEGG; osa:9272184; -.
DR eggNOG; ENOG502QT2R; Eukaryota.
DR HOGENOM; CLU_046484_1_0_1; -.
DR InParanoid; Q2QUS0; -.
DR OMA; CIHEKDD; -.
DR OrthoDB; 1333771at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR ExpressionAtlas; Q2QUS0; baseline and differential.
DR Genevisible; Q2QUS0; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 2.40.50.90; -; 1.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR Pfam; PF00565; SNase; 1.
DR SMART; SM00318; SNc; 1.
DR SUPFAM; SSF50199; SSF50199; 1.
DR PROSITE; PS50830; TNASE_3; 1.
PE 2: Evidence at transcript level;
KW Calcium; Cell membrane; Endonuclease; Hydrolase; Lipoprotein; Membrane;
KW Metal-binding; Myristate; Nuclease; Palmitate; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..495
FT /note="Probable staphylococcal-like nuclease CAN4"
FT /id="PRO_0000430203"
FT DOMAIN 297..470
FT /note="TNase-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT REGION 45..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 81..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..62
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..97
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 377
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT ACT_SITE 385
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT ACT_SITE 419
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT BINDING 310
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT BINDING 382
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT LIPID 11
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 495 AA; 56249 MW; 545DCB296113297B CRC64;
MGNILKCFIS CFDGEDEDGG SGHGRFPNSY PPVSSLHYQP LHLDDLQVPL SPPPPSTRQQ
QPPPRPALSR LHYQPLRLAD LQVPLSPPPP STRQQQLPPR PARPLGYHHG VLWPTIEITP
CDDFLNLEFT TKVCEGPQHH STPSRKTDLN RCIHEKDDGN SKPSYYWLTK NKDNHVVTPQ
TKRSPRPPSG VAALERDLVD FTRTFEVPEG LAQHVTSPMQ AQVTWYRKLL AAYKDIKYPP
KESADAAVLV AATLRGIERT NLEGILAFYG FPIPTISKEA SENHPSSIPK GVLFVLKTLP
VNAKCIVDGD GFTAYVDTLD PIELRQDCNA SCQSRNSKKQ KLWDKTAADL QISLENTGQK
KMFFGGREIL ARKYEIRLRG IDAPEIGMQY GKESQDALVK LIARKCVTLH VYGQDQFKRF
VCDIHCGGVF IQEQMLVNGH AWHFKNYDKR PQFAKWEKMA RDARQGLWAY DNLEKPWEWR
KNKRKASRHH NSDVR
