WNT7B_MOUSE
ID WNT7B_MOUSE Reviewed; 349 AA.
AC P28047; Q80US5;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Protein Wnt-7b;
DE Flags: Precursor;
GN Name=Wnt7b; Synonyms=Wnt-7b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2279700; DOI=10.1101/gad.4.12b.2319;
RA Gavin B.J., McMahon J.A., McMahon A.P.;
RT "Expression of multiple novel Wnt-1/int-1-related genes during fetal and
RT adult mouse development.";
RL Genes Dev. 4:2319-2332(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PORCN.
RX PubMed=10866835; DOI=10.1046/j.1432-1033.2000.01478.x;
RA Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.;
RT "The evolutionarily conserved porcupine gene family is involved in the
RT processing of the Wnt family.";
RL Eur. J. Biochem. 267:4300-4311(2000).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=11543617; DOI=10.1006/dbio.2001.0373;
RA Parr B.A., Cornish V.A., Cybulsky M.I., McMahon A.P.;
RT "Wnt7b regulates placental development in mice.";
RL Dev. Biol. 237:324-332(2001).
RN [5]
RP FUNCTION, INTERACTION WITH FZD1; FZD4 AND FZD10, AND SUBCELLULAR LOCATION.
RX PubMed=15923619; DOI=10.1128/mcb.25.12.5022-5030.2005;
RA Wang Z., Shu W., Lu M.M., Morrisey E.E.;
RT "Wnt7b activates canonical signaling in epithelial and vascular smooth
RT muscle cells through interactions with Fzd1, Fzd10, and LRP5.";
RL Mol. Cell. Biol. 25:5022-5030(2005).
RN [6]
RP FUNCTION.
RX PubMed=28803732; DOI=10.1016/j.neuron.2017.07.031;
RA Cho C., Smallwood P.M., Nathans J.;
RT "Reck and Gpr124 Are Essential Receptor Cofactors for Wnt7a/Wnt7b-specific
RT signaling in mammalian CNS angiogenesis and blood-brain barrier
RT regulation.";
RL Neuron 95:1056-1073(2017).
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors that functions in the canonical Wnt/beta-
CC catenin signaling pathway (PubMed:15923619, PubMed:28803732). Required
CC for normal fusion of the chorion and the allantois during placenta
CC development (PubMed:11543617). Required for central nervous system
CC (CNS) angiogenesis and blood-brain barrier regulation
CC (PubMed:28803732). {ECO:0000269|PubMed:11543617,
CC ECO:0000269|PubMed:15923619, ECO:0000269|PubMed:28803732}.
CC -!- SUBUNIT: Forms a soluble 1:1 complex with AFM; this prevents
CC oligomerization and is required for prolonged biological activity. The
CC complex with AFM may represent the physiological form in body fluids
CC (By similarity). Interacts with FZD1 and FZD10 (PubMed:15923619).
CC Interacts with FZD4 (in vitro) (PubMed:15923619). Interacts with PORCN
CC (PubMed:10866835). Interacts with glypican GPC3 (By similarity).
CC Interacts (via intrinsically disordered linker region) with RECK;
CC interaction with RECK confers ligand selectivity for Wnt7 in brain
CC endothelial cells and allows these cells to selectively respond to Wnt7
CC (By similarity). {ECO:0000250|UniProtKB:P56706,
CC ECO:0000269|PubMed:10866835, ECO:0000269|PubMed:15923619}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250|UniProtKB:P56706}. Secreted
CC {ECO:0000269|PubMed:15923619}.
CC -!- DEVELOPMENTAL STAGE: At 7.5 and 8.5 dpc, detected in extraembryonic
CC membranes and cells that form the chorionic plate.
CC {ECO:0000269|PubMed:11543617}.
CC -!- DOMAIN: The intrinsically disordered linker region is required for
CC recognition by RECK in brain endothelial cells.
CC {ECO:0000250|UniProtKB:P56706}.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors. Depalmitoleoylation leads to Wnt signaling pathway
CC inhibition. {ECO:0000250|UniProtKB:P27467,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR EMBL; M89802; AAA40571.1; -; mRNA.
DR EMBL; BC052018; AAH52018.2; -; mRNA.
DR CCDS; CCDS37171.1; -.
DR PIR; H36470; H36470.
DR RefSeq; NP_001157105.1; NM_001163633.1.
DR RefSeq; NP_001157106.1; NM_001163634.1.
DR RefSeq; NP_033554.3; NM_009528.3.
DR AlphaFoldDB; P28047; -.
DR SMR; P28047; -.
DR BioGRID; 204581; 7.
DR IntAct; P28047; 1.
DR STRING; 10090.ENSMUSP00000105051; -.
DR GlyGen; P28047; 3 sites.
DR iPTMnet; P28047; -.
DR PhosphoSitePlus; P28047; -.
DR MaxQB; P28047; -.
DR PaxDb; P28047; -.
DR PRIDE; P28047; -.
DR ProteomicsDB; 297561; -.
DR Antibodypedia; 27975; 102 antibodies from 22 providers.
DR DNASU; 22422; -.
DR Ensembl; ENSMUST00000109424; ENSMUSP00000105051; ENSMUSG00000022382.
DR GeneID; 22422; -.
DR KEGG; mmu:22422; -.
DR UCSC; uc007xdf.2; mouse.
DR CTD; 7477; -.
DR MGI; MGI:98962; Wnt7b.
DR VEuPathDB; HostDB:ENSMUSG00000022382; -.
DR eggNOG; KOG3913; Eukaryota.
DR GeneTree; ENSGT00940000158861; -.
DR InParanoid; P28047; -.
DR OMA; NSCSERT; -.
DR OrthoDB; 745245at2759; -.
DR PhylomeDB; P28047; -.
DR Reactome; R-MMU-3238698; WNT ligand biogenesis and trafficking.
DR BioGRID-ORCS; 22422; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Wnt7b; mouse.
DR PRO; PR:P28047; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; P28047; protein.
DR Bgee; ENSMUSG00000022382; Expressed in cortical plate and 241 other tissues.
DR ExpressionAtlas; P28047; baseline and differential.
DR Genevisible; P28047; MM.
DR GO; GO:0009986; C:cell surface; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:1902379; F:chemoattractant activity involved in axon guidance; IDA:ParkinsonsUK-UCL.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; IPI:MGI.
DR GO; GO:0048018; F:receptor ligand activity; ISO:MGI.
DR GO; GO:0060033; P:anatomical structure regression; IMP:MGI.
DR GO; GO:0001525; P:angiogenesis; IMP:MGI.
DR GO; GO:1902262; P:apoptotic process involved in blood vessel morphogenesis; IMP:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IDA:MGI.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0021846; P:cell proliferation in forebrain; IDA:BHF-UCL.
DR GO; GO:0022009; P:central nervous system vasculogenesis; IGI:MGI.
DR GO; GO:0036516; P:chemoattraction of dopaminergic neuron axon; IDA:ParkinsonsUK-UCL.
DR GO; GO:0060710; P:chorio-allantoic fusion; IMP:MGI.
DR GO; GO:0060560; P:developmental growth involved in morphogenesis; IMP:MGI.
DR GO; GO:0048568; P:embryonic organ development; IMP:MGI.
DR GO; GO:0060669; P:embryonic placenta morphogenesis; IMP:MGI.
DR GO; GO:0016332; P:establishment or maintenance of polarity of embryonic epithelium; IMP:MGI.
DR GO; GO:0021871; P:forebrain regionalization; IEP:UniProtKB.
DR GO; GO:0042592; P:homeostatic process; IMP:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0072061; P:inner medullary collecting duct development; IMP:MGI.
DR GO; GO:0060482; P:lobar bronchus development; IMP:MGI.
DR GO; GO:0030324; P:lung development; IMP:MGI.
DR GO; GO:0060428; P:lung epithelium development; IMP:MGI.
DR GO; GO:0060425; P:lung morphogenesis; IMP:MGI.
DR GO; GO:0060484; P:lung-associated mesenchyme development; IMP:MGI.
DR GO; GO:0072205; P:metanephric collecting duct development; IMP:MGI.
DR GO; GO:0072207; P:metanephric epithelium development; IMP:MGI.
DR GO; GO:0072236; P:metanephric loop of Henle development; IMP:MGI.
DR GO; GO:0003338; P:metanephros morphogenesis; IMP:MGI.
DR GO; GO:0050768; P:negative regulation of neurogenesis; IDA:BHF-UCL.
DR GO; GO:0045879; P:negative regulation of smoothened signaling pathway; IMP:MGI.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0031175; P:neuron projection development; ISO:MGI.
DR GO; GO:0048812; P:neuron projection morphogenesis; ISO:MGI.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI.
DR GO; GO:0001649; P:osteoblast differentiation; IDA:MGI.
DR GO; GO:0072060; P:outer medullary collecting duct development; IMP:MGI.
DR GO; GO:0032364; P:oxygen homeostasis; IMP:MGI.
DR GO; GO:1904938; P:planar cell polarity pathway involved in axon guidance; IMP:ParkinsonsUK-UCL.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:MGI.
DR GO; GO:0046330; P:positive regulation of JNK cascade; ISO:MGI.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; IDA:MGI.
DR GO; GO:0032536; P:regulation of cell projection size; ISO:MGI.
DR GO; GO:0072053; P:renal inner medulla development; IMP:MGI.
DR GO; GO:0072054; P:renal outer medulla development; IMP:MGI.
DR GO; GO:0051145; P:smooth muscle cell differentiation; IMP:MGI.
DR GO; GO:0048864; P:stem cell development; IDA:BHF-UCL.
DR GO; GO:0050808; P:synapse organization; IGI:MGI.
DR GO; GO:0060535; P:trachea cartilage morphogenesis; IMP:MGI.
DR GO; GO:0001944; P:vasculature development; IMP:MGI.
DR GO; GO:0016055; P:Wnt signaling pathway; IMP:MGI.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR013300; Wnt7.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01891; WNT7PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..349
FT /note="Protein Wnt-7b"
FT /id="PRO_0000041445"
FT REGION 238..266
FT /note="Disordered linker"
FT /evidence="ECO:0000250|UniProtKB:P56706"
FT LIPID 206
FT /note="O-palmitoleoyl serine; by PORCN"
FT /evidence="ECO:0000250|UniProtKB:P56704"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 295
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 73..84
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 123..131
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 133..152
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 200..214
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 202..209
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 278..309
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 294..304
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 308..348
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 324..339
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 326..336
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 331..332
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT CONFLICT 33
FT /note="G -> V (in Ref. 2; AAH52018)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 349 AA; 39302 MW; BDD82AB020DC680E CRC64;
MHRNFRKWIF YVFLCFGVLY VKLGALSSVV ALGANIICNK IPGLAPRQRA ICQSRPDAII
VIGEGAQMGI DECQHQFRFG RWNCSALGEK TVFGQELRVG SREAAFTYAI TAAGVAHAVT
AACSQGNLSN CGCDREKQGY YNQAEGWKWG GCSADVRYGI DFSRRFVDAR EIKKNARRLM
NLHNNEAGRK VLEDRMKLEC KCHGVSGSCT TKTCWTTLPK FREVGHLLKE KYNAAVQVEV
VRASRLRQPT FLRIKQLRSY QKPMETDLVY IEKSPNYCEE DAATGSVGTQ GRLCNRTSPG
ADGCDTMCCG RGYNTHQYTK VWQCNCKFHW CCFVKCNTCS ERTEVFTCK
