WNT8B_HUMAN
ID WNT8B_HUMAN Reviewed; 351 AA.
AC Q93098; O00771; Q5VX55; Q8WYK9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 3.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Protein Wnt-8b;
DE Flags: Precursor;
GN Name=WNT8B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9536085; DOI=10.1093/hmg/7.5.813;
RA Lako M., Lindsay S., Bullen P., Wilson D.I., Robson S.C., Strachan T.;
RT "A novel mammalian Wnt gene, WNT8B, shows brain-restricted expression in
RT early development, with sharply delimited expression boundaries in the
RT developing forebrain.";
RL Hum. Mol. Genet. 7:813-822(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fetal brain;
RX PubMed=11788899;
RA Saitoh T., Mine T., Katoh M.;
RT "Up-regulation of WNT8B mRNA in human gastric cancer.";
RL Int. J. Oncol. 20:343-348(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-317.
RX PubMed=8661156; DOI=10.1006/geno.1996.0374;
RA Lako M., Strachan T., Curtis A.R.J., Lindsay S.;
RT "Isolation and characterization of WNT8B, a novel human Wnt gene that maps
RT to 10q24.";
RL Genomics 35:386-388(1996).
RN [5]
RP VARIANT [LARGE SCALE ANALYSIS] GLN-53.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Ligand for members of the frizzled family of seven
CC transmembrane receptors. May play an important role in the development
CC and differentiation of certain forebrain structures, notably the
CC hippocampus. {ECO:0000269|PubMed:9536085}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- TISSUE SPECIFICITY: Expression is restricted to the brain, and more
CC specifically to the forebrain. {ECO:0000269|PubMed:9536085}.
CC -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC receptors (By similarity). Depalmitoleoylation leads to Wnt signaling
CC pathway inhibition (By similarity). {ECO:0000250|UniProtKB:P28026,
CC ECO:0000250|UniProtKB:P56704}.
CC -!- PTM: Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and
CC formation of large disulfide-bond oligomers, leading to inactivation of
CC WNT8B. {ECO:0000250|UniProtKB:P28026}.
CC -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR EMBL; Y11094; CAA71968.1; -; mRNA.
DR EMBL; Y11108; CAA71994.1; -; Genomic_DNA.
DR EMBL; AB073637; BAB83924.1; -; mRNA.
DR EMBL; AL359759; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL133352; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; X91940; CAA63017.1; -; mRNA.
DR CCDS; CCDS7494.1; -.
DR RefSeq; NP_003384.2; NM_003393.3.
DR AlphaFoldDB; Q93098; -.
DR SMR; Q93098; -.
DR BioGRID; 113316; 6.
DR IntAct; Q93098; 2.
DR STRING; 9606.ENSP00000340677; -.
DR GlyGen; Q93098; 2 sites.
DR iPTMnet; Q93098; -.
DR PhosphoSitePlus; Q93098; -.
DR BioMuta; WNT8B; -.
DR DMDM; 66774217; -.
DR MassIVE; Q93098; -.
DR PaxDb; Q93098; -.
DR PeptideAtlas; Q93098; -.
DR PRIDE; Q93098; -.
DR ProteomicsDB; 75724; -.
DR Antibodypedia; 17653; 227 antibodies from 31 providers.
DR DNASU; 7479; -.
DR Ensembl; ENST00000343737.6; ENSP00000340677.5; ENSG00000075290.8.
DR GeneID; 7479; -.
DR KEGG; hsa:7479; -.
DR MANE-Select; ENST00000343737.6; ENSP00000340677.5; NM_003393.4; NP_003384.2.
DR UCSC; uc001krb.4; human.
DR CTD; 7479; -.
DR DisGeNET; 7479; -.
DR GeneCards; WNT8B; -.
DR HGNC; HGNC:12789; WNT8B.
DR HPA; ENSG00000075290; Tissue enhanced (parathyroid gland, retina).
DR MIM; 601396; gene.
DR neXtProt; NX_Q93098; -.
DR OpenTargets; ENSG00000075290; -.
DR PharmGKB; PA37390; -.
DR VEuPathDB; HostDB:ENSG00000075290; -.
DR eggNOG; KOG3913; Eukaryota.
DR GeneTree; ENSGT00940000160584; -.
DR HOGENOM; CLU_033039_1_2_1; -.
DR InParanoid; Q93098; -.
DR OMA; FMHLEVF; -.
DR OrthoDB; 618621at2759; -.
DR PhylomeDB; Q93098; -.
DR TreeFam; TF105310; -.
DR PathwayCommons; Q93098; -.
DR Reactome; R-HSA-201681; TCF dependent signaling in response to WNT.
DR Reactome; R-HSA-3238698; WNT ligand biogenesis and trafficking.
DR Reactome; R-HSA-373080; Class B/2 (Secretin family receptors).
DR Reactome; R-HSA-4641262; Disassembly of the destruction complex and recruitment of AXIN to the membrane.
DR SignaLink; Q93098; -.
DR SIGNOR; Q93098; -.
DR BioGRID-ORCS; 7479; 9 hits in 1070 CRISPR screens.
DR GeneWiki; WNT8B; -.
DR GenomeRNAi; 7479; -.
DR Pharos; Q93098; Tbio.
DR PRO; PR:Q93098; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q93098; protein.
DR Bgee; ENSG00000075290; Expressed in adult mammalian kidney and 25 other tissues.
DR ExpressionAtlas; Q93098; baseline and differential.
DR Genevisible; Q93098; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0005109; F:frizzled binding; IBA:GO_Central.
DR GO; GO:0048018; F:receptor ligand activity; IDA:WormBase.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IDA:WormBase.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0071300; P:cellular response to retinoic acid; ISS:UniProtKB.
DR GO; GO:0048263; P:determination of dorsal identity; ISS:BHF-UCL.
DR GO; GO:0007369; P:gastrulation; ISS:BHF-UCL.
DR GO; GO:0007399; P:nervous system development; TAS:ProtInc.
DR GO; GO:0030182; P:neuron differentiation; ISS:UniProtKB.
DR GO; GO:0032355; P:response to estradiol; NAS:BHF-UCL.
DR GO; GO:0032526; P:response to retinoic acid; NAS:BHF-UCL.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR Gene3D; 3.30.2460.20; -; 1.
DR InterPro; IPR034311; Protein_Wnt-8B.
DR InterPro; IPR005817; Wnt.
DR InterPro; IPR013301; Wnt8.
DR InterPro; IPR043158; Wnt_C.
DR InterPro; IPR018161; Wnt_CS.
DR PANTHER; PTHR12027; PTHR12027; 1.
DR PANTHER; PTHR12027:SF94; PTHR12027:SF94; 1.
DR Pfam; PF00110; wnt; 1.
DR PRINTS; PR01892; WNT8PROTEIN.
DR PRINTS; PR01349; WNTPROTEIN.
DR SMART; SM00097; WNT1; 1.
DR PROSITE; PS00246; WNT1; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Lipoprotein; Reference proteome; Secreted; Signal; Wnt signaling pathway.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..351
FT /note="Protein Wnt-8b"
FT /id="PRO_0000041450"
FT LIPID 186
FT /note="O-palmitoleoyl serine"
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 259
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 54..65
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 104..112
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 114..132
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 180..194
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 182..189
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 256..294
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 272..287
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 291..333
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 309..324
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 311..321
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT DISULFID 316..317
FT /evidence="ECO:0000250|UniProtKB:P28026"
FT VARIANT 11
FT /note="C -> S (in dbSNP:rs3793771)"
FT /id="VAR_020310"
FT VARIANT 53
FT /note="E -> Q (in a colorectal cancer sample; somatic
FT mutation)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036288"
FT CONFLICT 230
FT /note="G -> A (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="R -> L (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 351 AA; 38721 MW; DD242070948813F8 CRC64;
MFLSKPSVYI CLFTCVLQLS HSWSVNNFLM TGPKAYLIYS SSVAAGAQSG IEECKYQFAW
DRWNCPERAL QLSSHGGLRS ANRETAFVHA ISSAGVMYTL TRNCSLGDFD NCGCDDSRNG
QLGGQGWLWG GCSDNVGFGE AISKQFVDAL ETGQDARAAM NLHNNEAGRK AVKGTMKRTC
KCHGVSGSCT TQTCWLQLPE FREVGAHLKE KYHAALKVDL LQGAGNSAAG RGAIADTFRS
ISTRELVHLE DSPDYCLENK TLGLLGTEGR ECLRRGRALG RWERRSCRRL CGDCGLAVEE
RRAETVSSCN CKFHWCCAVR CEQCRRRVTK YFCSRAERPR GGAAHKPGRK P
