ID   WNT8_THUTH              Reviewed;         128 AA.
AC   P28146;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Protein Wnt-8;
DE   Flags: Fragment;
GN   Name=wnt8;
OS   Thunnus thynnus (Atlantic bluefin tuna) (Scomber thynnus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Pelagiaria; Scombriformes; Scombridae; Thunnus.
OX   NCBI_TaxID=8237;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1534411; DOI=10.1073/pnas.89.11.5098;
RA   Sidow A.;
RT   "Diversification of the Wnt gene family on the ancestral lineage of
RT   vertebrates.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:5098-5102(1992).
CC   -!- FUNCTION: Ligand for members of the frizzled family of seven
CC       transmembrane receptors. Probable developmental protein. May be a
CC       signaling molecule which affects the development of discrete regions of
CC       tissues. Is likely to signal over only few cell diameters.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix.
CC   -!- PTM: Palmitoleoylation is required for efficient binding to frizzled
CC       receptors (By similarity). Depalmitoleoylation leads to Wnt signaling
CC       pathway inhibition (By similarity). {ECO:0000250|UniProtKB:P28026,
CC       ECO:0000250|UniProtKB:P56704}.
CC   -!- PTM: Proteolytic processing by tiki1 and tiki2 promotes oxidation and
CC       formation of large disulfide-bond oligomers, leading to inactivation of
CC       wnt8. {ECO:0000250|UniProtKB:P28026}.
CC   -!- SIMILARITY: Belongs to the Wnt family. {ECO:0000305}.
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DR   EMBL; M91308; AAA49625.1; -; Genomic_DNA.
DR   AlphaFoldDB; P28146; -.
DR   SMR; P28146; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0048513; P:animal organ development; IEA:UniProt.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2460.20; -; 1.
DR   InterPro; IPR034311; Protein_Wnt-8B.
DR   InterPro; IPR005817; Wnt.
DR   InterPro; IPR013301; Wnt8.
DR   InterPro; IPR043158; Wnt_C.
DR   PANTHER; PTHR12027; PTHR12027; 1.
DR   PANTHER; PTHR12027:SF94; PTHR12027:SF94; 1.
DR   Pfam; PF00110; wnt; 1.
DR   PRINTS; PR01892; WNT8PROTEIN.
DR   SMART; SM00097; WNT1; 1.
PE   3: Inferred from homology;
KW   Developmental protein; Disulfide bond; Extracellular matrix; Glycoprotein;
KW   Lipoprotein; Secreted; Wnt signaling pathway.
FT   CHAIN           <1..>128
FT                   /note="Protein Wnt-8"
FT                   /id="PRO_0000200659"
FT   LIPID           1
FT                   /note="O-palmitoleoyl serine"
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        93
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        71..109
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   DISULFID        87..102
FT                   /evidence="ECO:0000250|UniProtKB:P28026"
FT   NON_TER         1
FT   NON_TER         128
SQ   SEQUENCE   128 AA;  14293 MW;  849134882DE8C6B9 CRC64;
     SGSCTTQTCW LQLPEFREVG NYLKEKYHRA LKVDLLRGAG NSAASRGAIA ETFSSISRKE
     LVHLEDSPDY CLENRTLGLP GTEGRECLKK GKNLSKWEKR SCKRLCGECG LAVEERKAEM
     VSSCNCKF