WOR1_CANAL
ID WOR1_CANAL Reviewed; 785 AA.
AC Q5AP80; A0A1D8PET0; Q5JB38;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=White-opaque regulator 1;
GN Name=WOR1; Synonyms=EAP2, TOS9; OrderedLocusNames=CAALFM_C110150WA;
GN ORFNames=CaO19.12348, CaO19.4884;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION.
RX PubMed=16321041; DOI=10.1021/bp050236c;
RA Li F., Palecek S.P.;
RT "Identification of Candida albicans genes that induce Saccharomyces
RT cerevisiae cell adhesion and morphogenesis.";
RL Biotechnol. Prog. 21:1601-1609(2005).
RN [5]
RP FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16950924; DOI=10.1128/ec.00252-06;
RA Srikantha T., Borneman A.R., Daniels K.J., Pujol C., Wu W.,
RA Seringhaus M.R., Gerstein M., Yi S., Snyder M., Soll D.R.;
RT "TOS9 regulates white-opaque switching in Candida albicans.";
RL Eukaryot. Cell 5:1674-1687(2006).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=16899543; DOI=10.1073/pnas.0605138103;
RA Zordan R.E., Galgoczy D.J., Johnson A.D.;
RT "Epigenetic properties of white-opaque switching in Candida albicans are
RT based on a self-sustaining transcriptional feedback loop.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:12807-12812(2006).
RN [7]
RP FUNCTION.
RX PubMed=16905649; DOI=10.1073/pnas.0605270103;
RA Huang G., Wang H., Chou S., Nie X., Chen J., Liu H.;
RT "Bistable expression of WOR1, a master regulator of white-opaque switching
RT in Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:12813-12818(2006).
RN [8]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=17880264; DOI=10.1371/journal.pbio.0050256;
RA Zordan R.E., Miller M.G., Galgoczy D.J., Tuch B.B., Johnson A.D.;
RT "Interlocking transcriptional feedback loops control white-opaque switching
RT in Candida albicans.";
RL PLoS Biol. 5:E256-E256(2007).
RN [9]
RP FUNCTION.
RX PubMed=18551173; DOI=10.1371/journal.ppat.1000089;
RA Ramirez-Zavala B., Reuss O., Park Y.N., Ohlsen K., Morschhauser J.;
RT "Environmental induction of white-opaque switching in Candida albicans.";
RL PLoS Pathog. 4:E1000089-E1000089(2008).
RN [10]
RP FUNCTION.
RX PubMed=20735781; DOI=10.1111/j.1365-2958.2010.07331.x;
RA Lohse M.B., Johnson A.D.;
RT "Temporal anatomy of an epigenetic switch in cell programming: the white-
RT opaque transition of C. albicans.";
RL Mol. Microbiol. 78:331-343(2010).
RN [11]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=22095082; DOI=10.1534/genetics.111.134080;
RA Cain C.W., Lohse M.B., Homann O.R., Sil A., Johnson A.D.;
RT "A conserved transcriptional regulator governs fungal morphology in widely
RT diverged species.";
RL Genetics 190:511-521(2012).
RN [12]
RP FUNCTION.
RX PubMed=22621896; DOI=10.1091/mbc.e12-02-0094;
RA Du H., Guan G., Xie J., Cottier F., Sun Y., Jia W., Muhlschlegel F.A.,
RA Huang G.;
RT "The transcription factor Flo8 mediates CO2 sensing in the human fungal
RT pathogen Candida albicans.";
RL Mol. Biol. Cell 23:2692-2701(2012).
CC -!- FUNCTION: Master transcriptional regulator of the switch between 2
CC heritable states, the white and opaque states. These 2 cell types
CC differ in many characteristics, including cell structure, mating
CC competence, and virulence. Each state is heritable for many
CC generations, and switching between states occurs stochastically, at low
CC frequency. WOR1 Binds the intergenic regions upstream of the genes
CC encoding three additional transcriptional regulators of white-opaque
CC switching, CZF1, EFG1, and WOR2. Phenotypic switching from the white to
CC the opaque phase is a necessary step for mating. Plays a role in cell
CC adhesion and pseudohyphal growth. {ECO:0000269|PubMed:16321041,
CC ECO:0000269|PubMed:16899543, ECO:0000269|PubMed:16905649,
CC ECO:0000269|PubMed:16950924, ECO:0000269|PubMed:17880264,
CC ECO:0000269|PubMed:18551173, ECO:0000269|PubMed:20735781,
CC ECO:0000269|PubMed:22095082, ECO:0000269|PubMed:22621896}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16950924}.
CC -!- INDUCTION: Only expressed in opaque cells, in which it forms a positive
CC feedback loop since it binds its own DNA regulatory region and
CC activates its own transcription leading to the accumulation of high
CC levels of WOR1. {ECO:0000269|PubMed:16899543,
CC ECO:0000269|PubMed:16950924}.
CC -!- SIMILARITY: Belongs to the MIT1/WOR1 family. {ECO:0000305}.
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DR EMBL; CP017623; AOW26645.1; -; Genomic_DNA.
DR RefSeq; XP_723567.2; XM_718474.2.
DR PDB; 4QTJ; X-ray; 2.10 A; A=6-272.
DR PDB; 4QTK; X-ray; 2.99 A; A/B=6-272.
DR PDBsum; 4QTJ; -.
DR PDBsum; 4QTK; -.
DR AlphaFoldDB; Q5AP80; -.
DR SMR; Q5AP80; -.
DR BioGRID; 1217875; 105.
DR STRING; 237561.Q5AP80; -.
DR GeneID; 3634781; -.
DR KEGG; cal:CAALFM_C110150WA; -.
DR CGD; CAL0000193718; WOR1.
DR VEuPathDB; FungiDB:C1_10150W_A; -.
DR eggNOG; KOG4476; Eukaryota.
DR HOGENOM; CLU_356372_0_0_1; -.
DR InParanoid; Q5AP80; -.
DR OMA; VFVFIEQ; -.
DR OrthoDB; 1503680at2759; -.
DR PRO; PR:Q5AP80; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0000785; C:chromatin; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0003677; F:DNA binding; IDA:SGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:SGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0044406; P:adhesion of symbiont to host; IGI:SGD.
DR GO; GO:0007155; P:cell adhesion; IGI:SGD.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IMP:SGD.
DR GO; GO:1990277; P:parasexual reproduction with cellular fusion; IMP:SGD.
DR GO; GO:0036166; P:phenotypic switching; IMP:SGD.
DR GO; GO:1900241; P:positive regulation of phenotypic switching; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR GO; GO:1900239; P:regulation of phenotypic switching; IDA:SGD.
DR GO; GO:1900231; P:regulation of single-species biofilm formation on inanimate substrate; IMP:SGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:SGD.
DR GO; GO:0044011; P:single-species biofilm formation on inanimate substrate; IMP:SGD.
DR InterPro; IPR018608; Gti1/Pac2.
DR PANTHER; PTHR28027; PTHR28027; 1.
DR Pfam; PF09729; Gti1_Pac2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell adhesion; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Virulence.
FT CHAIN 1..785
FT /note="White-opaque regulator 1"
FT /id="PRO_0000420153"
FT REGION 96..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 516..616
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 697..785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..785
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 9..12
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 17..28
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 41..43
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 44..47
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 52..57
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 58..61
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 76..78
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 81..86
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 205..214
FT /evidence="ECO:0007829|PDB:4QTJ"
FT TURN 215..219
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 221..223
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 226..235
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 238..241
FT /evidence="ECO:0007829|PDB:4QTJ"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:4QTK"
FT HELIX 248..250
FT /evidence="ECO:0007829|PDB:4QTK"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:4QTJ"
FT HELIX 260..267
FT /evidence="ECO:0007829|PDB:4QTJ"
SQ SEQUENCE 785 AA; 83234 MW; 58A264075A9E2C4C CRC64;
MSNSSIVPTY NGYIHNTRDA LAVIQQVLDK QLEPVSRRPH ERERGVLIVS GSVFVFIEQS
SGIKRWTDGI SWSPSRIQGR FLVYGELDKK NLIDKDKKKK KKRKFGPDDE YDHNVNEPDY
TGGYGNHLHN DNRSHLNKNS RSGPMLLATG TGSITHTVIE NKPSSSASMI HSNVIPASSS
FLTDYRTSLG NGPMVSAAIS QNGLVKKTIT LTTTTKELHM EGKAEKQTIH LISYYSKQDI
DSGKLQRPSE SDLKHVQISP ALWTMVQENS LGGKAPIDDE ECFIVDGHNQ YTNVSYIQQQ
QQQHQLQHQP LLHHSSSVAG STTSIVNNSL SISNGGYGNN YSKNLSRSYN KYSNSQVSQL
SYMLPPQTES SSTATIASGA SVSVKREDDT NNGSNAPTGN ENQYVNAINH SHTSSYGGQG
YATDATGIAT PAYNSYSQAN TSINTSSQQQ QQLQQGQYGQ YVQYGVAPST ISGATSTNNN
SGNAPNIPQD VYYSHYTGFV QPHYPQYHIA TGNASDQYNT NAANHQYHSN NTTSSANNNS
SSRTTGVGSK RKPSIVSNST SGSVSGGNGN GNNYGYNSNS STSTNRPPAV STNTTSTTSG
GSSFSGPSSN ITTNSMSNNP WFNSSTNMAV NSSYITSSGG GNSHGGIGNN EYEPMPMTNN
SASIPAYYQQ HVPSHVGSAQ QHQSQQQVAG VGAPHIIHNH PYLHPTYGQG SNSASTGDNS
TPGGSSGSGS GGSGNNGAGG SSSVAATSGV TSSNTSGNIV TNGTLVAAGT DDAVGNSSGS
YYTGT
