ID   CAN5_RAT                Reviewed;         640 AA.
AC   Q8R4C0;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Calpain-5;
DE            EC=3.4.22.-;
GN   Name=Capn5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RA   Wang D.-S., McKinsey D., Pike B.R., DeFord M., Pineda J., Wang K.K.-W.,
RA   Hayes R.L.;
RT   "Cloning and normal expression profiling of rat calpain-5 by real-time
RT   PCR.";
RL   Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 277-283; 446-455 AND 566-572, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RA   Lubec G., Kang S.U., Lubec S.;
RL   Submitted (SEP-2007) to UniProtKB.
CC   -!- FUNCTION: Calcium-regulated non-lysosomal thiol-protease.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C2 family. {ECO:0000305}.
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DR   EMBL; AF484958; AAL92024.1; -; mRNA.
DR   AlphaFoldDB; Q8R4C0; -.
DR   SMR; Q8R4C0; -.
DR   STRING; 10116.ENSRNOP00000019370; -.
DR   MEROPS; C02.011; -.
DR   PhosphoSitePlus; Q8R4C0; -.
DR   SwissPalm; Q8R4C0; -.
DR   jPOST; Q8R4C0; -.
DR   PaxDb; Q8R4C0; -.
DR   RGD; 620084; Capn5.
DR   eggNOG; KOG0045; Eukaryota.
DR   InParanoid; Q8R4C0; -.
DR   PhylomeDB; Q8R4C0; -.
DR   BRENDA; 3.4.22.B25; 5301.
DR   Reactome; R-RNO-1474228; Degradation of the extracellular matrix.
DR   PRO; PR:Q8R4C0; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0009986; C:cell surface; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0045202; C:synapse; ISO:RGD.
DR   GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0060014; P:granulosa cell differentiation; IEP:RGD.
DR   GO; GO:0001553; P:luteinization; IEP:RGD.
DR   GO; GO:0001541; P:ovarian follicle development; IEP:RGD.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   CDD; cd04046; C2_Calpain; 1.
DR   CDD; cd00214; Calpain_III; 1.
DR   CDD; cd00044; CysPc; 1.
DR   Gene3D; 2.60.40.150; -; 1.
DR   InterPro; IPR033884; C2_Calpain.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR033883; C2_III.
DR   InterPro; IPR022684; Calpain_cysteine_protease.
DR   InterPro; IPR022682; Calpain_domain_III.
DR   InterPro; IPR022683; Calpain_III.
DR   InterPro; IPR036213; Calpain_III_sf.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR   Pfam; PF00168; C2; 1.
DR   Pfam; PF01067; Calpain_III; 1.
DR   Pfam; PF00648; Peptidase_C2; 1.
DR   PRINTS; PR00704; CALPAIN.
DR   SMART; SM00239; C2; 1.
DR   SMART; SM00720; calpain_III; 1.
DR   SMART; SM00230; CysPc; 1.
DR   SUPFAM; SSF49562; SSF49562; 1.
DR   SUPFAM; SSF49758; SSF49758; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS50004; C2; 1.
DR   PROSITE; PS50203; CALPAIN_CAT; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protease; Reference proteome;
KW   Thiol protease.
FT   CHAIN           1..640
FT                   /note="Calpain-5"
FT                   /id="PRO_0000207715"
FT   DOMAIN          26..343
FT                   /note="Calpain catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT   DOMAIN          499..617
FT                   /note="C2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   REGION          344..496
FT                   /note="Domain III"
FT   ACT_SITE        81
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        252
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        284
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   640 AA;  73065 MW;  098A6CDA7799BC61 CRC64;
     MFSCTKAYEY QNYSALKRAC LRRKVLFEDP HFPASDDSLY YKGTPGPTVR WKRPFDICDD
     PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWN PEKPDSYAGI
     FHFNFWRFGE WVDVVVDDRL PTVNNQLIYC HSNSKNEFWC APVEKAYAKL AGCYQALDGG
     NTADALVDFT GGVSEPIDLT EGDLATDEAK RSQLFERVLK VHSRGGLISA SIKAMTAADM
     ETRLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERV WTGPWSDTSE
     EWQKVSKSER EKMGVTVQDD GEFWMTYEDM CRYFTDIIKC RLINTSYLSI HKTWEEARLR
     GAWTRHEDPQ QNRSGGCINH KDTFFQNPQY IFEVKKPEDE VLICIQQRPK RSTRREGKGE
     NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TELPEGRYVI IPTTFEPGHT
     GELLLRVFTD VPSNCRELRL DEPPRTCWSS LCGYPQQVTQ VHVLGAAGLK DSSTGANSYV
     IIKCEGEKVR SAVQRGTSTP EYNVKGIFYR KKLSQPITVQ VWNNRVLKDE FLGQVHLKTA
     PDDLQDLHSL HLQDRSGRQP SDLPGIVAVR VLCSASLTAV