WOR2_CANAL
ID WOR2_CANAL Reviewed; 446 AA.
AC Q5ANB1; A0A1D8PK50;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=White-opaque regulator 2;
GN Name=WOR2; OrderedLocusNames=CAALFM_C305170WA;
GN ORFNames=CaO19.13413, CaO19.5992;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP DNA-BINDING.
RX PubMed=18629206; DOI=10.1002/cfg.492;
RA Maicas S., Moreno I., Nieto A., Gomez M., Sentandreu R., Valentin E.;
RT "In silico analysis for transcription factors with Zn(II)(2)C(6) binuclear
RT cluster DNA-binding domains in Candida albicans.";
RL Comp. Funct. Genomics 6:345-356(2005).
RN [5]
RP FUNCTION.
RX PubMed=17880264; DOI=10.1371/journal.pbio.0050256;
RA Zordan R.E., Miller M.G., Galgoczy D.J., Tuch B.B., Johnson A.D.;
RT "Interlocking transcriptional feedback loops control white-opaque switching
RT in Candida albicans.";
RL PLoS Biol. 5:E256-E256(2007).
RN [6]
RP FUNCTION.
RX PubMed=20735781; DOI=10.1111/j.1365-2958.2010.07331.x;
RA Lohse M.B., Johnson A.D.;
RT "Temporal anatomy of an epigenetic switch in cell programming: the white-
RT opaque transition of C. albicans.";
RL Mol. Microbiol. 78:331-343(2010).
RN [7]
RP INDUCTION.
RX PubMed=21592964; DOI=10.1074/jbc.m111.233569;
RA Singh R.P., Prasad H.K., Sinha I., Agarwal N., Natarajan K.;
RT "Cap2-HAP complex is a critical transcriptional regulator that has dual but
RT contrasting roles in regulation of iron homeostasis in Candida albicans.";
RL J. Biol. Chem. 286:25154-25170(2011).
RN [8]
RP FUNCTION.
RX PubMed=22621896; DOI=10.1091/mbc.e12-02-0094;
RA Du H., Guan G., Xie J., Cottier F., Sun Y., Jia W., Muhlschlegel F.A.,
RA Huang G.;
RT "The transcription factor Flo8 mediates CO2 sensing in the human fungal
RT pathogen Candida albicans.";
RL Mol. Biol. Cell 23:2692-2701(2012).
CC -!- FUNCTION: Transcriptional regulator of the switch between 2 heritable
CC states, the white and opaque states. These 2 cell types differ in many
CC characteristics, including cell structure, mating competence, and
CC virulence. Each state is heritable for many generations, and switching
CC between states occurs stochastically, at low frequency. WOR2 is
CC necessary for the stability of the opaque state phenotypic switching
CC from the white to the opaque phase is a necessary step for mating.
CC Plays a role in cell adhesion and pseudohyphal growth.
CC {ECO:0000269|PubMed:17880264, ECO:0000269|PubMed:20735781,
CC ECO:0000269|PubMed:22621896}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Expression is induced by HAP43 under low iron conditions.
CC {ECO:0000269|PubMed:21592964}.
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DR EMBL; CP017625; AOW28532.1; -; Genomic_DNA.
DR RefSeq; XP_723202.1; XM_718109.1.
DR AlphaFoldDB; Q5ANB1; -.
DR SMR; Q5ANB1; -.
DR BioGRID; 1218324; 1.
DR PRIDE; Q5ANB1; -.
DR GeneID; 3635230; -.
DR KEGG; cal:CAALFM_C305170WA; -.
DR CGD; CAL0000177394; WOR2.
DR VEuPathDB; FungiDB:C3_05170W_A; -.
DR HOGENOM; CLU_613927_0_0_1; -.
DR InParanoid; Q5ANB1; -.
DR OMA; LDFNEHF; -.
DR OrthoDB; 1607569at2759; -.
DR PRO; PR:Q5ANB1; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0070783; P:growth of unicellular organism as a thread of attached cells; IMP:CGD.
DR GO; GO:0070785; P:negative regulation of growth of unicellular organism as a thread of attached cells; IMP:CGD.
DR GO; GO:0036166; P:phenotypic switching; IMP:CGD.
DR GO; GO:1900241; P:positive regulation of phenotypic switching; IMP:CGD.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence; Zinc.
FT CHAIN 1..446
FT /note="White-opaque regulator 2"
FT /id="PRO_0000420155"
FT DNA_BIND 305..332
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 171..188
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..402
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 446 AA; 49106 MW; 9A12AD123EF57602 CRC64;
MTQLPSVSEL INRTGSIGSS SNITRVPPMT TTSATNTTTA ATATTVTSTT PRSENSYSPN
SPYSLPTRPS NTSLTNYSAG SGITVASSSF QFSQPSPGRS KHNSTSSQSS SGDSFQQHQS
NPSGVSMSSN TSPRTSIVQS MSSVPTPPST GPAPQQFVYQ ESQLPVQPPP QQQQLQQPPP
PPPQQQQHIY PQQQPNFPYH NNFVSPPTYT TAYPQYYYQP VMTPPHHQPH QQAPIHLANN
VSILQSQAVM QNAYPTTHYL QHVGPHHIYA NVNSQVYYQD MAREEANKAL INKRRIIKRR
TRTGCLTCRK RRIKCDERKP TCFNCERSKK SCLGYQDLSK LPPRKRNRDT SLDLPDGNQQ
QQQQQQNQQS IVGGAGNVSD EPHNETHQIT SISGSSTNSR NLDMMIPQSF RNNISSQPIN
FYGPVATTAS GASAVINRVS VADLLK
