WOX4_ARATH
ID WOX4_ARATH Reviewed; 251 AA.
AC Q6X7J9; Q9C632;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=WUSCHEL-related homeobox 4;
GN Name=WOX4; OrderedLocusNames=At1g46480; ORFNames=F2G19.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14711878; DOI=10.1242/dev.00963;
RA Haecker A., Gross-Hardt R., Geiges B., Sarkar A., Breuninger H.,
RA Herrmann M., Laux T.;
RT "Expression dynamics of WOX genes mark cell fate decisions during early
RT embryonic patterning in Arabidopsis thaliana.";
RL Development 131:657-668(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20729381; DOI=10.1105/tpc.110.076083;
RA Hirakawa Y., Kondo Y., Fukuda H.;
RT "TDIF peptide signaling regulates vascular stem cell proliferation via the
RT WOX4 homeobox gene in Arabidopsis.";
RL Plant Cell 22:2618-2629(2010).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=20044450; DOI=10.1104/pp.109.149641;
RA Ji J., Strable J., Shimizu R., Koenig D., Sinha N., Scanlon M.J.;
RT "WOX4 promotes procambial development.";
RL Plant Physiol. 152:1346-1356(2010).
RN [7]
RP FUNCTION.
RX PubMed=21926336; DOI=10.1105/tpc.111.087874;
RA Suer S., Agusti J., Sanchez P., Schwarz M., Greb T.;
RT "WOX4 imparts auxin responsiveness to cambium cells in Arabidopsis.";
RL Plant Cell 23:3247-3259(2011).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23578929; DOI=10.1242/dev.091314;
RA Etchells J.P., Provost C.M., Mishra L., Turner S.R.;
RT "WOX4 and WOX14 act downstream of the PXY receptor kinase to regulate plant
RT vascular proliferation independently of any role in vascular
RT organisation.";
RL Development 140:2224-2234(2013).
CC -!- FUNCTION: Promotes differentiation and/or maintenance of the vascular
CC procambium, the initial cells of the developing vasculature
CC (PubMed:20044450). Part of the TDIF-TDR-WOX4 signaling pathway that
CC plays a crucial role in the maintenance of the vascular meristem
CC organization during secondary growth (PubMed:20729381). Is required for
CC promoting the proliferation of procambial/cambial stem cells but not
CC for repressing their commitment to xylem differentiation in response to
CC the TDIF signal (PubMed:20729381). Acts redundantly with WOX14
CC downstream of the TDR/PXY receptor kinase to regulate procambial cell
CC proliferation and differentiation in vascular tissue, independently of
CC any role in vascular (PubMed:23578929). Acts as a cambium regulator in
CC the inflorescence stem (PubMed:21926336). Is required for auxin-
CC dependent cambium stimulation in the inflorescence stem
CC (PubMed:21926336). {ECO:0000269|PubMed:20044450,
CC ECO:0000269|PubMed:20729381, ECO:0000269|PubMed:21926336,
CC ECO:0000269|PubMed:23578929}.
CC -!- INTERACTION:
CC Q6X7J9; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-4459694, EBI-15191535;
CC Q6X7J9; Q9SVX5: DREB2F; NbExp=3; IntAct=EBI-4459694, EBI-15195807;
CC Q6X7J9; Q9ZVC9: FRS3; NbExp=3; IntAct=EBI-4459694, EBI-4448729;
CC Q6X7J9; Q9SDW0: GT-3A; NbExp=3; IntAct=EBI-4459694, EBI-9348720;
CC Q6X7J9; O82277: TCP10; NbExp=3; IntAct=EBI-4459694, EBI-3133327;
CC Q6X7J9; Q9S7W5: TCP13; NbExp=3; IntAct=EBI-4459694, EBI-4424877;
CC Q6X7J9; Q93Z00: TCP14; NbExp=3; IntAct=EBI-4459694, EBI-4424563;
CC Q6X7J9; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-4459694, EBI-4426144;
CC Q6X7J9; Q9M1U4: TCP16; NbExp=3; IntAct=EBI-4459694, EBI-15198627;
CC Q6X7J9; A1YKT1: TCP18; NbExp=3; IntAct=EBI-4459694, EBI-15192731;
CC Q6X7J9; Q8LPR5: TCP4; NbExp=3; IntAct=EBI-4459694, EBI-15192325;
CC Q6X7J9; Q9ZSI7: WRKY47; NbExp=3; IntAct=EBI-4459694, EBI-2367993;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:20729381}.
CC -!- TISSUE SPECIFICITY: Expressed in the vasculature of the whole plant
CC (roots, hypocotyls, cotyledons and leaves), trichomes and stomata
CC (PubMed:20729381). Expresse in the developing vascular bundles of root
CC and shoot lateral organs (PubMed:20044450).
CC {ECO:0000269|PubMed:20044450, ECO:0000269|PubMed:20729381}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:20729381). The double mutants wox4 and wox14 exhibit
CC reductions in vascular cell division (PubMed:23578929).
CC {ECO:0000269|PubMed:20729381, ECO:0000269|PubMed:23578929}.
CC -!- SIMILARITY: Belongs to the WUS homeobox family. {ECO:0000305}.
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DR EMBL; AY251396; AAP37134.1; -; mRNA.
DR EMBL; AC083835; AAG50620.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32127.1; -; Genomic_DNA.
DR EMBL; BT010692; AAR20749.1; -; mRNA.
DR EMBL; BT010974; AAR24752.1; -; mRNA.
DR PIR; F96511; F96511.
DR RefSeq; NP_175145.2; NM_103605.7.
DR AlphaFoldDB; Q6X7J9; -.
DR SMR; Q6X7J9; -.
DR BioGRID; 26338; 48.
DR IntAct; Q6X7J9; 45.
DR STRING; 3702.AT1G46480.1; -.
DR PaxDb; Q6X7J9; -.
DR PRIDE; Q6X7J9; -.
DR ProteomicsDB; 242721; -.
DR EnsemblPlants; AT1G46480.1; AT1G46480.1; AT1G46480.
DR GeneID; 841113; -.
DR Gramene; AT1G46480.1; AT1G46480.1; AT1G46480.
DR KEGG; ath:AT1G46480; -.
DR Araport; AT1G46480; -.
DR TAIR; locus:2825767; AT1G46480.
DR eggNOG; ENOG502R34Q; Eukaryota.
DR HOGENOM; CLU_106029_0_0_1; -.
DR InParanoid; Q6X7J9; -.
DR OMA; NDVIRRD; -.
DR OrthoDB; 1186068at2759; -.
DR PhylomeDB; Q6X7J9; -.
DR PRO; PR:Q6X7J9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6X7J9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:TAIR.
DR GO; GO:0010067; P:procambium histogenesis; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEP:TAIR.
DR GO; GO:0009888; P:tissue development; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR044186; WOX4.
DR PANTHER; PTHR47716; PTHR47716; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..251
FT /note="WUSCHEL-related homeobox 4"
FT /id="PRO_0000049370"
FT DNA_BIND 86..150
FT /note="Homeobox; WUS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 33..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..60
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..83
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 82..83
FT /note="Missing (in Ref. 2; AAG50620)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 28675 MW; 439373A6B8BE0C37 CRC64;
MKVHEFSNGF SSSWDQHDST SSLSLSCKRL RPLAPKLSGS PPSPPSSSSG VTSATFDLKN
FIRPDQTGPT KFEHKRDPPH QLETHPGGTR WNPTQEQIGI LEMLYKGGMR TPNAQQIEHI
TLQLGKYGKI EGKNVFYWFQ NHKARERQKQ KRNNLISLSC QSSFTTTGVF NPSVTMKTRT
SSSLDIMREP MVEKEELVEE NEYKRTCRSW GFENLEIENR RNKNSSTMAT TFNKIIDNVT
LELFPLHPEG R
