WR451_ORYSJ
ID WR451_ORYSJ Reviewed; 326 AA.
AC Q5W6D6; A0A0P0WKZ2;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Transcription factor WRKY45-1 {ECO:0000303|PubMed:19700558};
DE Short=OsWRKY45-1 {ECO:0000303|PubMed:19700558};
DE AltName: Full=OsWRKY45 {ECO:0000303|PubMed:15618416};
DE AltName: Full=WRKY transcription factor 45-2 {ECO:0000303|PubMed:19700558};
GN Name=WRKY45-1 {ECO:0000303|PubMed:19700558};
GN Synonyms=WRKY45 {ECO:0000303|PubMed:15618416};
GN OrderedLocusNames=Os05g0322900 {ECO:0000312|EMBL:BAS93366.1},
GN LOC_Os05g25770 {ECO:0000305};
GN ORFNames=OSJNBb0109A13.9 {ECO:0000312|EMBL:AAV44133.1},
GN P0018A03.1 {ECO:0000312|EMBL:AAV44172.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX DOI=10.1007/BF03185783;
RA Qiu Y., Jing S., Fu J., Li L., Yu D.;
RT "Cloning and analysis of expression profile of 13 WRKY genes in rice.";
RL Chin. Sci. Bull. 49:2159-2168(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19700558; DOI=10.1104/pp.109.145623;
RA Tao Z., Liu H., Qiu D., Zhou Y., Li X., Xu C., Wang S.;
RT "A pair of allelic WRKY genes play opposite roles in rice-bacteria
RT interactions.";
RL Plant Physiol. 151:936-948(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP TISSUE SPECIFICITY, AND INDUCTION BY ABSCISIC ACID.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [9]
RP INDUCTION.
RX PubMed=16528562; DOI=10.1007/s00299-006-0138-1;
RA Ryu H.-S., Han M., Lee S.-K., Cho J.-I., Ryoo N., Heu S., Lee Y.-H.,
RA Bhoo S.H., Wang G.-L., Hahn T.-R., Jeon J.-S.;
RT "A comprehensive expression analysis of the WRKY gene superfamily in rice
RT plants during defense response.";
RL Plant Cell Rep. 25:836-847(2006).
RN [10]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17601827; DOI=10.1105/tpc.106.046250;
RA Shimono M., Sugano S., Nakayama A., Jiang C.-J., Ono K., Toki S.,
RA Takatsuji H.;
RT "Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast
RT resistance.";
RL Plant Cell 19:2064-2076(2007).
RN [11]
RP FUNCTION.
RX PubMed=20459318; DOI=10.1094/mpmi-23-6-0791;
RA Jiang C.J., Shimono M., Sugano S., Kojima M., Yazawa K., Yoshida R.,
RA Inoue H., Hayashi N., Sakakibara H., Takatsuji H.;
RT "Abscisic acid interacts antagonistically with salicylic acid signaling
RT pathway in rice-Magnaporthe grisea interaction.";
RL Mol. Plant Microbe Interact. 23:791-798(2010).
RN [12]
RP FUNCTION, AND INDUCTION.
RX PubMed=21725029; DOI=10.1093/jxb/err144;
RA Tao Z., Kou Y., Liu H., Li X., Xiao J., Wang S.;
RT "OsWRKY45 alleles play different roles in abscisic acid signalling and salt
RT stress tolerance but similar roles in drought and cold tolerance in rice.";
RL J. Exp. Bot. 62:4863-4874(2011).
RN [13]
RP FUNCTION.
RX PubMed=21726399; DOI=10.1111/j.1364-3703.2011.00732.x;
RA Shimono M., Koga H., Akagi A., Hayashi N., Goto S., Sawada M., Kurihara T.,
RA Matsushita A., Sugano S., Jiang C.J., Kaku H., Inoue H., Takatsuji H.;
RT "Rice WRKY45 plays important roles in fungal and bacterial disease
RT resistance.";
RL Mol. Plant Pathol. 13:83-94(2012).
CC -!- FUNCTION: Transcriptional activator involved in defense responses
CC against pathogens (PubMed:17601827, PubMed:19700558, PubMed:21726399).
CC Acts as positive regulator of defense responses against the rice blast
CC fungus Magnaporthe oryzae (PubMed:17601827, PubMed:19700558,
CC PubMed:21726399). Acts through W-boxes, which are cis-elements that are
CC enriched in the promoters of several defense-related genes
CC (PubMed:17601827). Plays an important role in the benzothiadiazole-
CC induced disease resistance by mediating salicylic acid (SA) defense
CC signaling pathway, independently of the disease resistance gene
CC NPR1/NH1 (PubMed:17601827, PubMed:21726399). Acts as negative regulator
CC of defense responses against the bacterial blight Xanthomonas oryzae pv
CC oryzae (Xoo) and the bacterial streak Xanthomonas oryzae pv oryzicola
CC (Xoc) (PubMed:19700558). Acts downstream of abscisic acid (ABA)
CC signaling in response to the rice blast fungus (PubMed:20459318). ABA
CC is a negative regulator of defense responses that interacts
CC antagonistically with salicylic acid (SA) signaling pathway
CC (PubMed:20459318). Acts as negative regulator of ABA signaling that
CC suppresses growth of seedlings (PubMed:21725029). Does not seem to be
CC involved in the regulation of salt stress response (PubMed:21725029).
CC Acts as negative regulator of cold stress response (PubMed:21725029).
CC Acts as negative regulator of drought stress response
CC (PubMed:21725029). {ECO:0000269|PubMed:17601827,
CC ECO:0000269|PubMed:19700558, ECO:0000269|PubMed:20459318,
CC ECO:0000269|PubMed:21725029, ECO:0000269|PubMed:21726399}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223}.
CC -!- TISSUE SPECIFICITY: Expressed in aleurone cells.
CC {ECO:0000269|PubMed:15618416}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) in aleurone cells
CC (PubMed:15618416). Induced by salicylic acid (SA) and infection with
CC the rice blast fungus Magnaporthe oryzae (PubMed:16528562). Induced by
CC benzothiadiazole (BTH) and salicylic acid (SA) (PubMed:17601827).
CC Induced by cold stress (PubMed:21725029). Down-regulated by drought
CC stress (PubMed:21725029). {ECO:0000269|PubMed:15618416,
CC ECO:0000269|PubMed:16528562, ECO:0000269|PubMed:17601827,
CC ECO:0000269|PubMed:21725029}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants are compromised in benzothiadiazole-
CC induced resistance to the rice blast fungus Magnaporthe oryzae
CC (PubMed:17601827). Enhanced resistance to the bacterial blight
CC Xanthomonas oryzae pv oryzae (Xoo) and the bacterial streak Xanthomonas
CC oryzae pv oryzicola (Xoc) (PubMed:19700558). Enhanced resistance to
CC bacterial pathogens is associated with increased accumulation of
CC salicylic acid (SA) and jasmonate (JA) (PubMed:19700558).
CC {ECO:0000269|PubMed:17601827, ECO:0000269|PubMed:19700558}.
CC -!- MISCELLANEOUS: Plants overexpressing WRKY45 exhibit enhanced resistance
CC to the rice blast fungus Magnaporthe oryzae (PubMed:17601827,
CC PubMed:19700558). Plants overexpressing WRKY45 exhibit increased
CC susceptibility to the bacterial blight Xanthomonas oryzae pv oryzae
CC (Xoo) and the bacterial streak Xanthomonas oryzae pv oryzicola (Xoc)
CC (PubMed:19700558). {ECO:0000269|PubMed:17601827,
CC ECO:0000269|PubMed:19700558}.
CC -!- SIMILARITY: Belongs to the WRKY group III family. {ECO:0000305}.
CC -!- CAUTION: Rice plants contain two different alleles of WRKY45, WRKY45-1
CC (AC Q5W6D6) and WRKY45-2 (AC B8AWM1), which play similar and opposite
CC roles in defense response, response to abscisic acid (ABA) and response
CC to abiotic stresses. {ECO:0000305|PubMed:19700558,
CC ECO:0000305|PubMed:21725029}.
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DR EMBL; AY870611; AAW63720.1; -; mRNA.
DR EMBL; GQ331930; ACX37128.1; -; Genomic_DNA.
DR EMBL; GQ331931; ACX37129.1; -; Genomic_DNA.
DR EMBL; GQ331932; ACX37130.1; -; Genomic_DNA.
DR EMBL; AC136227; AAV44133.1; -; Genomic_DNA.
DR EMBL; AC134346; AAV44172.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17106.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93366.1; -; Genomic_DNA.
DR EMBL; AK066255; BAG89885.1; -; mRNA.
DR EMBL; AK103959; BAG96342.1; -; mRNA.
DR RefSeq; XP_015637594.1; XM_015782108.1.
DR AlphaFoldDB; Q5W6D6; -.
DR SMR; Q5W6D6; -.
DR STRING; 4530.OS05T0322900-01; -.
DR PaxDb; Q5W6D6; -.
DR PRIDE; Q5W6D6; -.
DR EnsemblPlants; Os05t0322900-01; Os05t0322900-01; Os05g0322900.
DR GeneID; 4338413; -.
DR Gramene; Os05t0322900-01; Os05t0322900-01; Os05g0322900.
DR KEGG; osa:4338413; -.
DR eggNOG; ENOG502RYCZ; Eukaryota.
DR HOGENOM; CLU_071943_0_0_1; -.
DR InParanoid; Q5W6D6; -.
DR OMA; NRKANCR; -.
DR OrthoDB; 1376049at2759; -.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR PlantReactome; R-OSA-6788019; Salicylic acid signaling.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR ExpressionAtlas; Q5W6D6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IDA:UniProtKB.
DR GO; GO:1900425; P:negative regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0080148; P:negative regulation of response to water deprivation; IDA:UniProtKB.
DR GO; GO:0080151; P:positive regulation of salicylic acid mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009409; P:response to cold; IDA:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31282; PTHR31282; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Plant defense; Reference proteome;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..326
FT /note="Transcription factor WRKY45-1"
FT /id="PRO_0000453487"
FT DNA_BIND 112..180
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 67..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 326 AA; 33988 MW; 42C0EC6BB9EB6E3C CRC64;
MTSSMSPAPA PAYAQVMEDM EKGKELAAQL QGLLRDSPEA GRFVDQILHT FSRAMRALDK
AAVSAAGGEG SEVQSEVTCG GGASAGGKRK APAADRKANC RRRTQQSSGN SVVVKNLDDG
QAWRKYGQKE IQNSKHPKAY FRCTHKYDQL CTAQRQVQRC DDDPASYRVT YIGEHTCRDP
ATAPIIAAHV IHQVAAGDND DGCGGLQAGS RLISFVAAPA APVDAAAAPT TSTITTVTAP
GPLLQPLKVE GGVGSSDQEE VLSSLTPGSS AARGGGGGGG VAGPFGPDQG DVTSSLHWSY
DAVAGMEFFK NDEVVFDLDD IMGLSF
