WR52N_ARATH
ID WR52N_ARATH Reviewed; 1378 AA.
AC E1B328; Q0WWA0; Q689Y9; Q8GZ83; Q9FH83; Q9FH84;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Disease resistance protein RRS1 {ECO:0000303|PubMed:11842188};
DE AltName: Full=Disease resistance protein RCH2;
DE AltName: Full=Disease resistance protein SLH1;
DE AltName: Full=Probable WRKY transcription factor 52;
DE AltName: Full=Protein RPS4-homolog;
DE AltName: Full=Protein SENSITIVE TO LOW HUMIDITY 1 {ECO:0000303|PubMed:16146526};
DE AltName: Full=Resistance to Colletotrichum higginsianum 2 protein;
DE AltName: Full=Resistance to Ralstonia solanacearum 1 protein {ECO:0000303|PubMed:11842188};
DE AltName: Full=WRKY DNA-binding protein 52;
GN Name=RRS1 {ECO:0000303|PubMed:11842188};
GN Synonyms=RCH2, RRS1-R {ECO:0000303|PubMed:11842188}, RSH4,
GN SLH1 {ECO:0000303|PubMed:16146526}, WRKY52;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ADM88042.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RC STRAIN=cv. Nd-1;
RX PubMed=11842188; DOI=10.1073/pnas.032485099;
RA Deslandes L., Olivier J., Theulieres F., Hirsch J., Feng D.X.,
RA Bittner-Eddy P., Beynon J., Marco Y.;
RT "Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred
RT by the recessive RRS1-R gene, a member of a novel family of resistance
RT genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:2404-2409(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LEU-1224, AND FUNCTION.
RC STRAIN=cv. No-0;
RX PubMed=16146526; DOI=10.1111/j.1365-313x.2005.02500.x;
RA Noutoshi Y., Ito T., Seki M., Nakashita H., Yoshida S., Marco Y.,
RA Shirasu K., Shinozaki K.;
RT "A single amino acid insertion in the WRKY domain of the Arabidopsis TIR-
RT NBS-LRR-WRKY-type disease resistance protein SLH1 (sensitive to low
RT humidity 1) causes activation of defense responses and hypersensitive cell
RT death.";
RL Plant J. 43:873-888(2005).
RN [3]
RP INTERACTION WITH POPP2, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nd-1;
RX PubMed=12788974; DOI=10.1073/pnas.1230660100;
RA Deslandes L., Olivier J., Peeters N., Feng D.X., Khounlotham M.,
RA Boucher C., Somssich I., Genin S., Marco Y.;
RT "Physical interaction between RRS1-R, a protein conferring resistance to
RT bacterial wilt, and PopP2, a type III effector targeted to the plant
RT nucleus.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:8024-8029(2003).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element. Acts also as a disease resistance protein involved in
CC resistance to fungal and bacterial pathogens, including R.solanacearum,
CC P.syringae pv. tomato and C.higginsianum. RRS1 mediated resistance
CC depends on salicylic acid and NDR1 (AC O48915).
CC {ECO:0000250|UniProtKB:P0DKH5, ECO:0000269|PubMed:11842188,
CC ECO:0000305}.
CC -!- SUBUNIT: Interacts with PopP2, a R.solanacearum type III effector.
CC {ECO:0000269|PubMed:12788974}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12788974}. Cytoplasm
CC {ECO:0000305|PubMed:12788974}. Note=The nuclear localization is only
CC detected upon interaction with PopP2. {ECO:0000269|PubMed:12788974}.
CC -!- MISCELLANEOUS: Ecotypes susceptible to C.higginsianum or
CC R.solanacearum, such as cv. Columbia, contain a protein with a
CC premature stop codon while the longer allele found in cv. Nd-1, cv.
CC Wassilewskija or cv. RLD confers resistance. {ECO:0000305}.
CC -!- MISCELLANEOUS: The slh1-induced phenotype (severely stunted growth when
CC grown under conditions of low humidity) is suppressed under conditions
CC of high humidity and high temperature. {ECO:0000269|PubMed:16146526}.
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DR EMBL; HQ170631; ADM88042.1; -; mRNA.
DR EMBL; AX103687; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR EMBL; AX103688; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR EMBL; AX103691; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR EMBL; AB188827; BAD38678.1; -; Genomic_DNA.
DR AlphaFoldDB; E1B328; -.
DR SMR; E1B328; -.
DR ExpressionAtlas; E1B328; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 2.20.25.80; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 2.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; DNA-binding; Leucine-rich repeat;
KW Nucleotide-binding; Nucleus; Plant defense; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..1378
FT /note="Disease resistance protein RRS1"
FT /id="PRO_0000431360"
FT DOMAIN 5..146
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 170..421
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 498..522
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 535..553
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 554..575
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 577..598
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 621..646
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 665..688
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 742..766
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 768..793
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 831..854
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT DNA_BIND 1204..1272
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 1300..1321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 988..1005
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT BINDING 179..186
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00499"
FT MUTAGEN 1224
FT /note="L->LL: In slh1; loss of DNA-binding activity and
FT constitutive defense activation."
FT /evidence="ECO:0000269|PubMed:16146526"
SQ SEQUENCE 1378 AA; 156108 MW; DB20F541F0BB73F0 CRC64;
MTNCEKDEEF VCISCVEEVR YSFVSHLSEA LRRKGINNVV VDVDIDDLLF KESQAKIEKA
GVSVMVLPGN CDPSEVWLDK FAKVLECQRN NKDQAVVSVL YGDSLLRDQW LSELDFRGLS
RIHQSRKECS DSILVEEIVR DVYETHFYVG RIGIYSKLLE IENMVNKQPI GIRCVGIWGM
PGIGKTTLAK AVFDQMSSAF DASCFIEDYD KSIHEKGLYC LLEEQLLPGN DATIMKLSSL
RDRLNSKRVL VVLDDVCNAL VAESFLEGFD WLGPGSLIII TSRDKQVFRL CGINQIYEVQ
GLNEKEARQL FLLSASIMED MGEQNLHELS VRVISYANGN PLAISVYGRE LKGKKKLSEM
ETAFLKLKRR PPFKIVDAFK SSYDTLSDNE KNIFLDIACF FQGENVNYVI QLLEGCGFFP
HVEIDVLVDK CLVTISENRV WLHKLTQDIG REIINGETVQ IERRRRLWEP WSIKYLLEYN
EHKANGEPKT TFKRAQGSEE IEGLFLDTSN LRFDLQPSAF KNMLNLRLLK IYCSNPEVHP
VINFPTGSLH SLPNELRLLH WENYPLKSLP QNFDPRHLVE INMPYSQLQK LWGGTKNLEM
LRTIRLCHSQ HLVDIDDLLK AENLEVIDLQ GCTRLQNFPA AGRLLRLRVV NLSGCIKIKS
VLEIPPNIEK LHLQGTGILA LPVSTVKPNH RELVNFLTEI PGLSEASKLE RLTSLLESNS
SCQDLGKLIC LELKDCSCLQ SLPNMANLDL NVLDLSGCSS LNSIQGFPRF LKQLYLGGTA
IREVPQLPQS LEILNAHGSC LRSLPNMANL EFLKVLDLSG CSELETIQGF PRNLKELYFA
GTTLREVPQL PLSLEVLNAH GSDSEKLPMH YKFNNFFDLS QQVVNDFFLK ALTYVKHIPR
GYTQELINKA PTFSFSAPSH TNQNATFDLQ PGSSVMTRLN HSWRNTLVGF GMLVEVAFPE
DYCDATDVGI SCVCRWSNKE GRSCRIERNF HCWAPGKVVP KVRKDHTFVF SDVNMRPSTG
EGNDPDIWAG LVVFEFFPIN QQTKCLNDRF TVTRCGVRVI NVATGNTSLE NISLVLSLDP
VEVSGYEVLR VSYDDLQEMD KVLFLYIASL FNDEDVDFVA PLIAGIDLDV SSGLKVLADV
SLISVSSNGE IVMHSLQRQM GKEILHGQSM LLSDCESSMT ENLSDVPKKE KKHRESKVKK
VVSIPAIDEG DLWTWRKYGQ KDILGSRFPR GYYRCAYKFT HGCKATKQVQ RSETDSNMLA
ITYLSEHNHP RPTKRKALAD STRSTSSSIC SAITTSASSR VFQNKDEPNQ PHLPSSSTPP
RNAAVLFKMT DMEEFQDNME VDNDVVDTRT LALFPEFQHQ PEEEDPWSTF FDDYNFYF
