WRK10_ARATH
ID WRK10_ARATH Reviewed; 485 AA.
AC Q9LG05; Q1PFK1; Q8VWQ3;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Probable WRKY transcription factor 10;
DE AltName: Full=Protein MINISEED 3;
DE AltName: Full=WRKY DNA-binding protein 10;
GN Name=WRKY10; Synonyms=MINI3; OrderedLocusNames=At1g55600; ORFNames=F20N2.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Ulker B., Kushnir S., Somssich I.E.;
RT "Arabidopsis thaliana transcription factor WRKY10.";
RL Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND MUTAGENESIS OF GLU-344.
RX PubMed=16293693; DOI=10.1073/pnas.0508418102;
RA Luo M., Dennis E.S., Berger F., Peacock W.J., Chaudhury A.;
RT "MINISEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucine-rich
RT repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:17531-17536(2005).
RN [6]
RP INTERACTION WITH IKU1.
RX PubMed=20545893; DOI=10.1111/j.1365-313x.2010.04271.x;
RA Wang A., Garcia D., Zhang H., Feng K., Chaudhury A., Berger F.,
RA Peacock W.J., Dennis E.S., Luo M.;
RT "The VQ motif protein IKU1 regulates endosperm growth and seed size in
RT Arabidopsis.";
RL Plant J. 63:670-679(2010).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element (By similarity). Modulates seed size by negatively
CC regulating the cellularization of syncytial endosperm
CC (PubMed:16293693). {ECO:0000250|UniProtKB:Q9SI37,
CC ECO:0000269|PubMed:16293693}.
CC -!- SUBUNIT: Interacts with IKU1. {ECO:0000269|PubMed:20545893}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:16293693}.
CC -!- TISSUE SPECIFICITY: Expressed in male gametophytes (pollen) and in the
CC endosperm of fertilized ovules. {ECO:0000269|PubMed:16293693}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the endosperm of embryo from the two
CC nuclei stage to the late globular embryo stage, including the endosperm
CC cellularization time. {ECO:0000269|PubMed:16293693}.
CC -!- SIMILARITY: Belongs to the WRKY group I family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79511.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY071851; AAL61861.1; -; mRNA.
DR EMBL; AC002328; AAF79511.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33270.1; -; Genomic_DNA.
DR EMBL; DQ446362; ABE65713.1; -; mRNA.
DR RefSeq; NP_175956.1; NM_104436.2.
DR AlphaFoldDB; Q9LG05; -.
DR SMR; Q9LG05; -.
DR BioGRID; 27234; 1.
DR STRING; 3702.AT1G55600.1; -.
DR PaxDb; Q9LG05; -.
DR PRIDE; Q9LG05; -.
DR ProteomicsDB; 232452; -.
DR EnsemblPlants; AT1G55600.1; AT1G55600.1; AT1G55600.
DR GeneID; 842009; -.
DR Gramene; AT1G55600.1; AT1G55600.1; AT1G55600.
DR KEGG; ath:AT1G55600; -.
DR Araport; AT1G55600; -.
DR TAIR; locus:2020467; AT1G55600.
DR eggNOG; ENOG502QU86; Eukaryota.
DR HOGENOM; CLU_027756_0_0_1; -.
DR InParanoid; Q9LG05; -.
DR OMA; DYATTTI; -.
DR OrthoDB; 847761at2759; -.
DR PhylomeDB; Q9LG05; -.
DR PRO; PR:Q9LG05; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LG05; baseline and differential.
DR Genevisible; Q9LG05; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009960; P:endosperm development; IMP:TAIR.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31221; PTHR31221; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..485
FT /note="Probable WRKY transcription factor 10"
FT /id="PRO_0000133652"
FT DNA_BIND 301..366
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 43..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..293
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..417
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..263
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..396
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 398..414
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 332
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 337
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 361
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 363
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT MUTAGEN 344
FT /note="E->K: In mini3-1; reduced seed size and earlier
FT endosperm cellularization."
FT /evidence="ECO:0000269|PubMed:16293693"
SQ SEQUENCE 485 AA; 54552 MW; 3D96CE29CC9BFAC0 CRC64;
MSDFDENFIE MTSYWAPPSS PSPRTILAML EQTDNGLNPI SEIFPQESLP RDHTDQSGQR
SGLRERLAAR VGFNLPTLNT EENMSPLDAF FRSSNVPNSP VVAISPGFSP SALLHTPNMV
SDSSQIIPPS SATNYGPLEM VETSGEDNAA MMMFNNDLPY QPYNVDLPSL EVFDDIATEE
SFYIPSYEPH VDPIGTPLVT SFESELVDDA HTDIISIEDS ESEDGNKDDD DEDFQYEDED
EDQYDQDQDV DEDEEEEKDE DNVALDDPQP PPPKRRRYEV SNMIGATRTS KTQRIILQME
SDEDNPNDGY RWRKYGQKVV KGNPNPRSYF KCTNIECRVK KHVERGADNI KLVVTTYDGI
HNHPSPPARR SNSSSRNRSA GATIPQNQND RTSRLGRAPP TPTPPTPPPS SYTPEEMRPF
SSLATEIDLT EVYMTGISML PNIPVYENSG FMYQNDEPTM NAMPDGSDVY DGIMERLYFK
FGVDM
