WRK18_ARATH
ID WRK18_ARATH Reviewed; 310 AA.
AC Q9C5T4; O81768; Q944H8;
DT 15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2002, sequence version 2.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=WRKY transcription factor 18;
DE AltName: Full=WRKY DNA-binding protein 18;
DE Short=AtWRKY18;
GN Name=WRKY18; OrderedLocusNames=At4g31800; ORFNames=F28M20.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE, AND INDUCTION.
RX PubMed=11449049; DOI=10.2307/3871384;
RA Yu D., Chen C., Chen Z.;
RT "Evidence for an important role of WRKY DNA binding proteins in the
RT regulation of NPR1 gene expression.";
RL Plant Cell 13:1527-1540(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=12068113; DOI=10.1104/pp.001057;
RA Chen C., Chen Z.;
RT "Potentiation of developmentally regulated plant defense response by
RT AtWRKY18, a pathogen-induced Arabidopsis transcription factor.";
RL Plant Physiol. 129:706-716(2002).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element. Positively modulates defense-related gene expression
CC and disease resistance. {ECO:0000269|PubMed:12068113}.
CC -!- INTERACTION:
CC Q9C5T4; Q42403: TRX3; NbExp=3; IntAct=EBI-1993349, EBI-449157;
CC Q9C5T4; Q9C5T4: WRKY18; NbExp=4; IntAct=EBI-1993349, EBI-1993349;
CC Q9C5T4; Q9SAH7: WRKY40; NbExp=7; IntAct=EBI-1993349, EBI-1993363;
CC Q9C5T4; Q9SK33: WRKY60; NbExp=7; IntAct=EBI-1993349, EBI-2112777;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9C5T4-1; Sequence=Displayed;
CC -!- INDUCTION: By salicylic acid and pathogens.
CC {ECO:0000269|PubMed:11449049, ECO:0000269|PubMed:12068113}.
CC -!- MISCELLANEOUS: Constitutive expression at high level causes severe
CC abnormality in plant growth.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19743.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79898.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF224698; AAK28308.1; -; mRNA.
DR EMBL; AL031004; CAA19743.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161579; CAB79898.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85960.1; -; Genomic_DNA.
DR EMBL; AF428421; AAL16190.1; -; mRNA.
DR EMBL; AY125557; AAM78067.1; -; mRNA.
DR EMBL; AY088048; AAM65594.1; -; mRNA.
DR RefSeq; NP_567882.1; NM_119329.4. [Q9C5T4-1]
DR AlphaFoldDB; Q9C5T4; -.
DR SMR; Q9C5T4; -.
DR BioGRID; 14594; 13.
DR IntAct; Q9C5T4; 10.
DR STRING; 3702.AT4G31800.1; -.
DR iPTMnet; Q9C5T4; -.
DR PaxDb; Q9C5T4; -.
DR PRIDE; Q9C5T4; -.
DR ProteomicsDB; 234174; -. [Q9C5T4-1]
DR EnsemblPlants; AT4G31800.1; AT4G31800.1; AT4G31800. [Q9C5T4-1]
DR GeneID; 829308; -.
DR Gramene; AT4G31800.1; AT4G31800.1; AT4G31800. [Q9C5T4-1]
DR KEGG; ath:AT4G31800; -.
DR Araport; AT4G31800; -.
DR TAIR; locus:2124874; AT4G31800.
DR eggNOG; ENOG502QR7M; Eukaryota.
DR InParanoid; Q9C5T4; -.
DR OMA; TSSCKRD; -.
DR PhylomeDB; Q9C5T4; -.
DR PRO; PR:Q9C5T4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9C5T4; baseline and differential.
DR Genevisible; Q9C5T4; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0042742; P:defense response to bacterium; IEP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEP:TAIR.
DR GO; GO:0031347; P:regulation of defense response; IMP:TAIR.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IMP:TAIR.
DR GO; GO:0009751; P:response to salicylic acid; IEP:TAIR.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..310
FT /note="WRKY transcription factor 18"
FT /id="PRO_0000133660"
FT DNA_BIND 170..236
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 82..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 124..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 271
FT /note="D -> N (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 34766 MW; 762E6B520AC3FF8D CRC64;
MDGSSFLDIS LDLNTNPFSA KLPKKEVSVL ASTHLKRKWL EQDESASELR EELNRVNSEN
KKLTEMLARV CESYNELHNH LEKLQSRQSP EIEQTDIPIK KRKQDPDEFL GFPIGLSSGK
TENSSSNEDH HHHHQQHEQK NQLLSCKRPV TDSFNKAKVS TVYVPTETSD TSLTVKDGFQ
WRKYGQKVTR DNPSPRAYFR CSFAPSCPVK KKVQRSAEDP SLLVATYEGT HNHLGPNASE
GDATSQGGSS TVTLDLVNGC HRLALEKNER DNTMQEVLIQ QMASSLTKDS KFTAALAAAI
SGRLMEQSRT
