WRK19_ARATH
ID WRK19_ARATH Reviewed; 1895 AA.
AC Q9SZ67;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Probable WRKY transcription factor 19;
DE AltName: Full=WRKY DNA-binding protein 19;
GN Name=WRKY19; OrderedLocusNames=At4g12020; ORFNames=F16J13.90;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element. May act also as a disease resistance protein with a
CC serine/threonine-protein kinase activity (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q9SZ67; Q9FVU9: CSN5B; NbExp=3; IntAct=EBI-2356712, EBI-697501;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SZ67-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the disease resistance X-TIR-NB-LRR-X family.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AL049638; CAB40943.1; -; Genomic_DNA.
DR EMBL; AL161533; CAB78245.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83085.1; -; Genomic_DNA.
DR PIR; T06609; T06609.
DR RefSeq; NP_001118968.1; NM_001125496.2. [Q9SZ67-1]
DR AlphaFoldDB; Q9SZ67; -.
DR SMR; Q9SZ67; -.
DR BioGRID; 12108; 2.
DR IntAct; Q9SZ67; 2.
DR STRING; 3702.AT4G12020.2; -.
DR PaxDb; Q9SZ67; -.
DR PRIDE; Q9SZ67; -.
DR ProteomicsDB; 234218; -. [Q9SZ67-1]
DR EnsemblPlants; AT4G12020.2; AT4G12020.2; AT4G12020. [Q9SZ67-1]
DR GeneID; 826810; -.
DR Gramene; AT4G12020.2; AT4G12020.2; AT4G12020. [Q9SZ67-1]
DR KEGG; ath:AT4G12020; -.
DR Araport; AT4G12020; -.
DR TAIR; locus:2118116; AT4G12020.
DR eggNOG; KOG0198; Eukaryota.
DR eggNOG; KOG4204; Eukaryota.
DR HOGENOM; CLU_002119_0_0_1; -.
DR OrthoDB; 189439at2759; -.
DR PRO; PR:Q9SZ67; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZ67; baseline and differential.
DR Genevisible; Q9SZ67; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.20.1160.11; -; 1.
DR Gene3D; 2.20.25.80; -; 2.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003822; PAH.
DR InterPro; IPR036600; PAH_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF02671; PAH; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF03106; WRKY; 2.
DR SMART; SM00774; WRKY; 2.
DR SUPFAM; SSF118290; SSF118290; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47762; SSF47762; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS51477; PAH; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS50104; TIR; 1.
DR PROSITE; PS50811; WRKY; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; DNA-binding; Kinase;
KW Leucine-rich repeat; Nucleotide-binding; Nucleus; Plant defense;
KW Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription;
KW Transcription regulation; Transferase.
FT CHAIN 1..1895
FT /note="Probable WRKY transcription factor 19"
FT /id="PRO_0000133661"
FT DOMAIN 291..371
FT /note="PAH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00810"
FT DOMAIN 666..808
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 800..1087
FT /note="NB-ARC"
FT REPEAT 1206..1227
FT /note="LRR 1"
FT REPEAT 1228..1249
FT /note="LRR 2"
FT REPEAT 1259..1281
FT /note="LRR 3"
FT REPEAT 1282..1304
FT /note="LRR 4"
FT REPEAT 1306..1328
FT /note="LRR 5"
FT REPEAT 1329..1351
FT /note="LRR 6"
FT REPEAT 1352..1371
FT /note="LRR 7"
FT REPEAT 1373..1395
FT /note="LRR 8"
FT REPEAT 1397..1419
FT /note="LRR 9"
FT REPEAT 1421..1442
FT /note="LRR 10"
FT DOMAIN 1626..1877
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DNA_BIND 462..526
FT /note="WRKY 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT DNA_BIND 635..700
FT /note="WRKY 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 1..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 408..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..538
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 580..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1562..1583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 580..598
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1758
FT /evidence="ECO:0000250"
FT BINDING 844..851
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 1632..1640
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 1654
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 1895 AA; 210322 MW; 1C19D3EE164C9363 CRC64;
MSEKEELPLT LTSIGAATAT SDYHQRVGSS GEGISSSSSD VDPRFMQNSP TGLMISQSSS
MCTVPPGMAA TPPISSGSGL SQQLNNSSSS KLCQVEGCQK GARDASGRCI SHGGGRRCQK
PDCQKGAEGK TVYCKAHGGG RRCEYLGCTK GAEGSTDFCI AHGGGRRCNH EDCTRSAWGR
TEFCVKHGGG ARCKTYGCGK SASGPLPFCR AHGGGKKCSH EDCTGFARGR SGLCLMHGGG
KRCQRENCTK SAEGLSGLCI SHGGGRRCQS IGCTKGAKGS KMFCKACITK RPLTIDGGGN
MGGVTTGDAL NYLKAVKDKF EDSEKYDTFL EVLNDCKHQG VDTSGVIARL KDLFKGHDDL
LLGFNTYLSK EYQITILPED DFPIDFLDKV EGPYEMTYQQ AQTVQANANM QPQTEYPSSS
AVQSFSSGQP QIPTSAPDSS LLAKSNTSGI TIIEHMSQQP LNVDKQVNDG YNWQKYGQKK
VKGSKFPLSY YKCTYLGCPS KRKVERSLDG QVAEIVYKDR HNHEPPNQGK DGSTTYLSGS
STHINCMSSE LTASQFSSNK TKIEQQEAAS LATTIEYMSE ASDNEEDSNG ETSEGEKDED
EPEPKRRITE VQVSELADAS DRTVREPRVI FQTTSEVDNL DDGYRWRKYG QKVVKGNPYP
RFSSSKDYDV VIRYGRADIS NEDFISHLRA SLCRRGISVY EKFNEVDALP KCRVLIIVLT
STYVPSNLLN ILEHQHTEDR VVYPIFYRLS PYDFVCNSKN YERFYLQDEP KKWQAALKEI
TQMPGYTLTD KSESELIDEI VRDALKVLCS ADKVNMIGMD MQVEEILSLL CIESLDVRSI
GIWGTVGIGK TTIAEEIFRK ISVQYETCVV LKDLHKEVEV KGHDAVRENF LSEVLEVEPH
VIRISDIKTS FLRSRLQRKR ILVILDDVND YRDVDTFLGT LNYFGPGSRI IMTSRNRRVF
VLCKIDHVYE VKPLDIPKSL LLLDRGTCQI VLSPEVYKTL SLELVKFSNG NPQVLQFLSS
IDREWNKLSQ EVKTTSPIYI PGIFEKSCCG LDDNERGIFL DIACFFNRID KDNVAMLLDG
CGFSAHVGFR GLVDKSLLTI SQHNLVDMLS FIQATGREIV RQESADRPGD RSRLWNADYI
RHVFINDTGT SAIEGIFLDM LNLKFDANPN VFEKMCNLRL LKLYCSKAEE KHGVSFPQGL
EYLPSKLRLL HWEYYPLSSL PKSFNPENLV ELNLPSSCAK KLWKGKKARF CTTNSSLEKL
KKMRLSYSDQ LTKIPRLSSA TNLEHIDLEG CNSLLSLSQS ISYLKKLVFL NLKGCSKLEN
IPSMVDLESL EVLNLSGCSK LGNFPEISPN VKELYMGGTM IQEIPSSIKN LVLLEKLDLE
NSRHLKNLPT SIYKLKHLET LNLSGCISLE RFPDSSRRMK CLRFLDLSRT DIKELPSSIS
YLTALDELLF VDSRRNSPVV TNPNANSTEL MPSESSKLEI LGTPADNEVV VGGTVEKTRG
IERTPTILVK SREYLIPDDV VAVGGDIKGL RPPVLQLQPA MKLSHIPRGS TWDFVTHFAP
PETVAPPSSS SEAREEEVET EETGAMFIPL GDKETCSFTV NKGDSSRTIS NTSPIYASEG
SFITCWQKGQ LLGRGSLGSV YEGISADGDF FAFKEVSLLD QGSQAHEWIQ QVEGGIALLS
QLQHQNIVRY RGTTKDESNL YIFLELVTQG SLRKLYQRNQ LGDSVVSLYT RQILDGLKYL
HDKGFIHRNI KCANVLVDAN GTVKLADFGL AKVMSLWRTP YWNWMAPEVI LNPKDYDGYG
TPADIWSLGC TVLEMLTGQI PYSDLEIGTA LYNIGTGKLP KIPDILSLDA RDFILTCLKV
NPEERPTAAE LLNHPFVNMP LPSSGSGSVS SLLRG
