WRK24_ORYSJ
ID WRK24_ORYSJ Reviewed; 555 AA.
AC Q6IEQ7; Q7XAA7; Q94D89;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=WRKY transcription factor WRKY24 {ECO:0000303|PubMed:15047897};
DE Short=OsWRKY24 {ECO:0000303|PubMed:15047897};
GN Name=WRKY24 {ECO:0000303|PubMed:15047897};
GN OrderedLocusNames=Os01g0826400 {ECO:0000312|EMBL:BAF06589.1};
GN ORFNames=OsJ_03928 {ECO:0000312|EMBL:EAZ14002.1},
GN OSNPB_010826400 {ECO:0000312|EMBL:BAS75019.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Yao Q., Peng R., Xiong A.;
RT "Isolation of rice WRKY protein through W-box bait vector by modified yeast
RT one-hybrid system method.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX DOI=10.1007/BF03185783;
RA Qiu Y., Jing S., Fu J., Li L., Yu D.;
RT "Cloning and analysis of expression profile of 13 WRKY genes in rice.";
RL Chin. Sci. Bull. 49:2159-2168(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=15047897; DOI=10.1104/pp.103.034967;
RA Zhang Z.-L., Xie Z., Zou X., Casaretto J., Ho T.-H.D., Shen Q.J.;
RT "A rice WRKY gene encodes a transcriptional repressor of the gibberellin
RT signaling pathway in aleurone cells.";
RL Plant Physiol. 134:1500-1513(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP FUNCTION, INDUCTION BY ABSCISIC ACID AND GIBBERELLIC ACID, TISSUE
RP SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [11]
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY ABSCISIC ACID, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. M-104, and cv. Tainung 67;
RX PubMed=19199048; DOI=10.1007/s11103-009-9463-4;
RA Zhang Z.L., Shin M., Zou X., Huang J., Ho T.H., Shen Q.J.;
RT "A negative regulator encoded by a rice WRKY gene represses both abscisic
RT acid and gibberellins signaling in aleurone cells.";
RL Plant Mol. Biol. 70:139-151(2009).
RN [12]
RP FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=26025535; DOI=10.1016/j.plantsci.2015.04.014;
RA Zhang L., Gu L., Ringler P., Smith S., Rushton P.J., Shen Q.J.;
RT "Three WRKY transcription factors additively repress abscisic acid and
RT gibberellin signaling in aleurone cells.";
RL Plant Sci. 236:214-222(2015).
CC -!- FUNCTION: Transcription activator (PubMed:26025535). Interacts
CC specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring
CC elicitor-responsive cis-acting element (PubMed:19199048,
CC PubMed:26025535). Negative regulator of both gibberellic acid (GA) and
CC abscisic acid (ABA) signaling in aleurone cells, probably by
CC interfering with GAM1, via the specific repression of GA- and ABA-
CC induced promoters (PubMed:15618416, PubMed:19199048, PubMed:26025535).
CC {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:19199048,
CC ECO:0000269|PubMed:26025535}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:19199048}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6IEQ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6IEQ7-2; Sequence=VSP_058455;
CC -!- TISSUE SPECIFICITY: Expressed in aleurone cells (PubMed:15618416).
CC Mostly expressed in aleurone layers and leaves, and, to a lower extent,
CC in roots, panicles and embryos (PubMed:26025535).
CC {ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:26025535}.
CC -!- DEVELOPMENTAL STAGE: In dry seeds, expressed in aleurone cells and
CC embryos. Levels drop rapidly but transiently in the embryos of imbibed
CC seeds. {ECO:0000269|PubMed:19199048}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) in aleurone cells, embryos,
CC roots and leaves (PubMed:15618416, PubMed:19199048). Slightly down-
CC regulated by gibberellic acid (GA) (PubMed:15618416). Accumulates in
CC response to jasmonic acid (MeJA) (By similarity).
CC {ECO:0000250|UniProtKB:Q6B6R4, ECO:0000269|PubMed:15618416,
CC ECO:0000269|PubMed:19199048}.
CC -!- DOMAIN: The WRKY domain is required to bind DNA.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
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DR EMBL; AY341849; AAQ20908.1; -; mRNA.
DR EMBL; AY870608; AAW63717.1; -; mRNA.
DR EMBL; BK005027; DAA05089.1; -; Genomic_DNA.
DR EMBL; AP003315; BAB61266.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06589.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS75019.1; -; Genomic_DNA.
DR EMBL; CM000138; EAZ14002.1; -; Genomic_DNA.
DR EMBL; AK107199; BAG97993.1; -; mRNA.
DR RefSeq; XP_015619871.1; XM_015764385.1. [Q6IEQ7-1]
DR AlphaFoldDB; Q6IEQ7; -.
DR SMR; Q6IEQ7; -.
DR STRING; 4530.OS01T0826400-01; -.
DR PaxDb; Q6IEQ7; -.
DR PRIDE; Q6IEQ7; -.
DR EnsemblPlants; Os01t0826400-01; Os01t0826400-01; Os01g0826400. [Q6IEQ7-1]
DR GeneID; 4327518; -.
DR Gramene; Os01t0826400-01; Os01t0826400-01; Os01g0826400. [Q6IEQ7-1]
DR KEGG; osa:4327518; -.
DR eggNOG; ENOG502QRXJ; Eukaryota.
DR HOGENOM; CLU_012086_5_0_1; -.
DR InParanoid; Q6IEQ7; -.
DR OMA; NNMFSRT; -.
DR OrthoDB; 782615at2759; -.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IDA:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IDA:UniProtKB.
DR Gene3D; 2.20.25.80; -; 2.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31221; PTHR31221; 1.
DR Pfam; PF03106; WRKY; 2.
DR SMART; SM00774; WRKY; 2.
DR SUPFAM; SSF118290; SSF118290; 2.
DR PROSITE; PS50811; WRKY; 2.
PE 2: Evidence at transcript level;
KW Abscisic acid signaling pathway; Activator; Alternative splicing;
KW DNA-binding; Gibberellin signaling pathway; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..555
FT /note="WRKY transcription factor WRKY24"
FT /id="PRO_0000436950"
FT DNA_BIND 214..278
FT /note="WRKY 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT DNA_BIND 379..444
FT /note="WRKY 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 133..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..248
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..555
FT /note="Transcription repression of gibberellic acid (GA)-
FT induced promoters"
FT /evidence="ECO:0000250|UniProtKB:Q6QHD1"
FT REGION 514..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 253..259
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000303|PubMed:15618416"
FT COMPBIAS 159..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..219
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..298
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 306..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..198
FT /note="Missing (in isoform 2)"
FT /id="VSP_058455"
SQ SEQUENCE 555 AA; 59306 MW; B1936C9DCB313733 CRC64;
MTTSSSGSVE TSANSRLGTF SFASASFTDL LGGNAGAGGG GVSRYKAMTP PSLPLSPPPV
SPSSFFNSPI GMNQADFLGS PVLLTSSIFP SPTTGAFASQ HFDWRPEVAA AQSADQGGKD
EQRNSYSDFS FQTAPASEEA VRTTTFQPPV PPAPLGDEAY RSQQQQQPWG YQQQPAGMDA
GANAASFGAA PFQATSSEMA PQVQGGGGYS QPQSQRRSSD DGYNWRKYGQ KQVKGSENPR
SYYKCTFPNC PTKKKVERSL DGQITEIVYK GTHNHAKPQN TRRNSGSSAA QVLQSGGDMS
EHSFGGMSGT AATPENSSAS FGDDEIRVGS PRAGNGGGDE FDDDEPDSKR WRKDGDGEGI
SMAGNRTVRE PRVVVQTMSD IDILDDGYRW RKYGQKVVKG NPNPRSYYKC TTAGCPVRKH
VERASHDLRA VITTYEGKHN HDVPAARGSA ALYRPAPPAA AATSSHPYLP NQPPPMSYQP
TGPQPYALRP DGFGGQGPFG GVVGGSSFGG FSGFDDARGS YMSQHQQQQR QNDAMHASRA
KEEPGDDMFF QNSLY
