WRK28_ORYSJ
ID WRK28_ORYSJ Reviewed; 396 AA.
AC Q0DAJ3; A0A0N7KMI2; Q67WY4; Q6IEQ3;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=WRKY transcription factor WRKY28 {ECO:0000303|PubMed:15618416};
DE Short=OsWRKY28 {ECO:0000303|PubMed:15618416};
GN Name=WRKY28 {ECO:0000303|PubMed:15618416};
GN OrderedLocusNames=Os06g0649000 {ECO:0000312|EMBL:BAF20130.1};
GN ORFNames=OsJ_22168 {ECO:0000312|EMBL:EEE66124.1},
GN OSJNBa0062J02.29 {ECO:0000312|EMBL:BAD37335.1},
GN OSNPB_060649000 {ECO:0000312|EMBL:BAS98888.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15047897; DOI=10.1104/pp.103.034967;
RA Zhang Z.-L., Xie Z., Zou X., Casaretto J., Ho T.-H.D., Shen Q.J.;
RT "A rice WRKY gene encodes a transcriptional repressor of the gibberellin
RT signaling pathway in aleurone cells.";
RL Plant Physiol. 134:1500-1513(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 32-396.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=15629062; DOI=10.1186/1471-2148-5-1;
RA Zhang Y., Wang L.;
RT "The WRKY transcription factor superfamily: its origin in eukaryotes and
RT expansion in plants.";
RL BMC Evol. Biol. 5:1-1(2005).
RN [8]
RP GENE FAMILY.
RX PubMed=16106749; DOI=10.1093/dnares/12.1.9;
RA Wu K.-L., Guo Z.-J., Wang H.-H., Li J.;
RT "The WRKY family of transcription factors in rice and Arabidopsis and their
RT origins.";
RL DNA Res. 12:9-26(2005).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [10]
RP GENE FAMILY.
RX PubMed=19772648; DOI=10.1186/1471-2229-9-120;
RA Berri S., Abbruscato P., Faivre-Rampant O., Brasileiro A.C., Fumasoni I.,
RA Satoh K., Kikuchi S., Mizzi L., Morandini P., Pe M.E., Piffanelli P.;
RT "Characterization of WRKY co-regulatory networks in rice and Arabidopsis.";
RL BMC Plant Biol. 9:120-120(2009).
RN [11]
RP FUNCTION.
RX PubMed=21961049; DOI=10.1007/s12284-010-9039-6;
RA Peng Y., Bartley L.E., Canlas P., Ronald P.C.;
RT "OsWRKY IIa transcription factors modulate rice innate immunity.";
RL Rice 3:36-42(2010).
RN [12]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY MAGNAPORTHE ORYZAE.
RC STRAIN=cv. Nipponbare;
RX PubMed=21726398; DOI=10.1111/j.1364-3703.2011.00731.x;
RA Delteil A., Blein M., Faivre-Rampant O., Guellim A., Estevan J., Hirsch J.,
RA Bevitori R., Michel C., Morel J.B.;
RT "Building a mutant resource for the study of disease resistance in rice
RT reveals the pivotal role of several genes involved in defence.";
RL Mol. Plant Pathol. 13:72-82(2012).
RN [13]
RP FUNCTION, INDUCTION BY BIOTIC ELICITORS, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=23462973; DOI=10.1007/s11103-013-0032-5;
RA Chujo T., Miyamoto K., Shimogawa T., Shimizu T., Otake Y., Yokotani N.,
RA Nishizawa Y., Shibuya N., Nojiri H., Yamane H., Minami E., Okada K.;
RT "OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate
RT immune responses in rice against rice blast fungus.";
RL Plant Mol. Biol. 82:23-37(2013).
CC -!- FUNCTION: Transcription repressor. Interacts specifically with the W
CC box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive
CC cis-acting element (PubMed:23462973). Regulates, probably indirectly,
CC the activation of defense-related genes during defense response (By
CC similarity). Modulates plant innate immunity against X.oryzae pv.
CC oryzae (Xoo) (PubMed:21961049). Regulates negatively the basal defense
CC responses to the compatible fungus M.oryzae (PubMed:23462973,
CC PubMed:21726398). {ECO:0000250|UniProtKB:Q6QHD1,
CC ECO:0000269|PubMed:21726398, ECO:0000269|PubMed:21961049,
CC ECO:0000269|PubMed:23462973}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:23462973}.
CC -!- INDUCTION: Induced by biotic elicitors (e.g. fungal chitin
CC oligosaccharide) (PubMed:23462973). Accumulates in response to M.oryzae
CC (PubMed:21726398). {ECO:0000269|PubMed:21726398,
CC ECO:0000269|PubMed:23462973}.
CC -!- DOMAIN: The WRKY domain is required to bind DNA.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- DISRUPTION PHENOTYPE: Increased basal resistance to rice blast mediated
CC by M.oryzae. {ECO:0000269|PubMed:21726398}.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD37335.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAF20130.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAS98888.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAS98889.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=DAA05093.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EEE66124.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BK005031; DAA05093.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP003517; BAD37335.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008212; BAF20130.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014962; BAS98888.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014962; BAS98889.1; ALT_INIT; Genomic_DNA.
DR EMBL; CM000143; EEE66124.1; ALT_INIT; Genomic_DNA.
DR EMBL; AK106282; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK119644; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q0DAJ3; -.
DR SMR; Q0DAJ3; -.
DR STRING; 4530.OS06T0649000-01; -.
DR PaxDb; Q0DAJ3; -.
DR PRIDE; Q0DAJ3; -.
DR eggNOG; ENOG502QR7M; Eukaryota.
DR InParanoid; Q0DAJ3; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:1905034; P:regulation of antifungal innate immune response; IMP:UniProtKB.
DR GO; GO:0010200; P:response to chitin; IEP:UniProtKB.
DR GO; GO:0009620; P:response to fungus; IEP:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; DNA-binding; Nucleus; Plant defense; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..396
FT /note="WRKY transcription factor WRKY28"
FT /id="PRO_0000436952"
FT DNA_BIND 245..311
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 157..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 306..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 101..135
FT /evidence="ECO:0000255"
FT MOTIF 222..229
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 157..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 331
FT /note="P -> R (in Ref. 6; AK119644)"
FT /evidence="ECO:0000305"
FT CONFLICT 340
FT /note="V -> D (in Ref. 6; AK106282)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 42643 MW; E2671ADE3BB7186A CRC64;
MAKMLPPPSQ SVPSRPPSWL YIPPRRRHGT FTSSCAFRLS PSSPSSPPPP VLDFQYIQFM
DSWIEQTSLS LDLNVGLPST ARRSSAPAAP IKVLVEENFL SFKKDHEVEA LEAELRRASE
ENKKLTEMLR AVVAKYTELQ GQVNDMMSAA AAAAVNAGNH QSSTSEGGSV SPSRKRIRSV
DSLDDAAHHR KPSPPFVAAA AAAAYASPDQ MECTSAAAAA AAKRVVREDC KPKVSKRFVH
ADPSDLSLVV KDGYQWRKYG QKVTKDNPCP RAYFRCSFAP ACPVKKKVQR SADDNTVLVA
TYEGEHNHAQ PPHHDAGSKT AAAAKHSQHQ PPPSAAAAVV RQQQEQAAAA GPSTEVAARK
NLAEQMAATL TRDPGFKAAL VTALSGRILE LSPTKN
