WRK35_ARATH
ID WRK35_ARATH Reviewed; 427 AA.
AC O64747;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Probable WRKY transcription factor 35;
DE AltName: Full=WRKY DNA-binding protein 35;
GN Name=WRKY35; OrderedLocusNames=At2g34830; ORFNames=F19I3.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Ulker B., Kushnir S., Somssich I.E.;
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O64747-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O64747-2; Sequence=VSP_007125, VSP_007126, VSP_007127;
CC -!- SIMILARITY: Belongs to the WRKY group II-e family. {ECO:0000305}.
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DR EMBL; AF404863; AAK96201.1; -; mRNA.
DR EMBL; AC004238; AAC12823.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09027.1; -; Genomic_DNA.
DR EMBL; BT002781; AAO22609.1; -; mRNA.
DR PIR; T00465; T00465.
DR RefSeq; NP_181029.1; NM_129036.2. [O64747-1]
DR AlphaFoldDB; O64747; -.
DR SMR; O64747; -.
DR BioGRID; 3394; 1.
DR STRING; 3702.AT2G34830.1; -.
DR PaxDb; O64747; -.
DR PRIDE; O64747; -.
DR ProteomicsDB; 234345; -. [O64747-1]
DR EnsemblPlants; AT2G34830.1; AT2G34830.1; AT2G34830. [O64747-1]
DR GeneID; 818048; -.
DR Gramene; AT2G34830.1; AT2G34830.1; AT2G34830. [O64747-1]
DR KEGG; ath:AT2G34830; -.
DR Araport; AT2G34830; -.
DR TAIR; locus:2044777; AT2G34830.
DR eggNOG; ENOG502QU8N; Eukaryota.
DR HOGENOM; CLU_029232_3_0_1; -.
DR InParanoid; O64747; -.
DR PhylomeDB; O64747; -.
DR PRO; PR:O64747; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64747; baseline and differential.
DR Genevisible; O64747; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR32096; PTHR32096; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..427
FT /note="Probable WRKY transcription factor 35"
FT /id="PRO_0000133677"
FT DNA_BIND 209..275
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..37
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..66
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_007125"
FT VAR_SEQ 67..76
FT /note="SMKDPLIHLP -> MPLDVLGRTH (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_007126"
FT VAR_SEQ 235..238
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_007127"
SQ SEQUENCE 427 AA; 46280 MW; 1CCDC53C5CE4EE5D CRC64;
MDNFQGDLTD VVRGIGSGHV SPSPGPPEGP SPSSMSPPPT SDLHVEFPSA ATSASCLANP
FGDPFVSMKD PLIHLPASYI SGAGDNKSNK SFAIFPKIFE DDHIKSQCSV FPRIKISQSN
NIHDASTCNS PAITVSSAAV AASPWGMINV NTTNSPRNCL LVDNNNNTSS CSQVQISSSP
RNLGIKRRKS QAKKVVCIPA PAAMNSRSSG EVVPSDLWAW RKYGQKPIKG SPYPRGYYRC
SSSKGCSARK QVERSRTDPN MLVITYTSEH NHPWPTQRNA LAGSTRSSSS SSLNPSSKSS
TAAATTSPSS RVFQNNSSKD EPNNSNLPSS STHPPFDAAA IKEENVEERQ EKMEFDYNDV
ENTYRPELLQ EFQHQPEDFF ADLDELEGDS LTMLLSHSSG GGNMENKTTI PDVFSDFFDD
DESSRSL
