WRK44_ARATH
ID WRK44_ARATH Reviewed; 429 AA.
AC Q9ZUU0;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-APR-2003, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=WRKY transcription factor 44;
DE AltName: Full=Protein TRANSPARENT TESTA GLABRA 2;
DE AltName: Full=WRKY DNA-binding protein 44;
GN Name=WRKY44; Synonyms=TTG2; OrderedLocusNames=At2g37260; ORFNames=F3G5.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 45-429, CONCEPTUAL TRANSLATION
RP OF 1-44, FUNCTION, INDUCTION, AND MUTANT TTG2-2.
RC STRAIN=cv. Landsberg erecta; TISSUE=Root;
RX PubMed=12084832; DOI=10.1105/tpc.001404;
RA Johnson C.S., Kolevski B., Smyth D.R.;
RT "TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene of
RT Arabidopsis, encodes a WRKY transcription factor.";
RL Plant Cell 14:1359-1375(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 81-429.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Ulker B., Kushnir S., Somssich I.E.;
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element. Regulates trichome development, production of mucilage
CC and tannin in seed coats, and maybe root hair development.
CC {ECO:0000269|PubMed:12084832}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9SI37}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9ZUU0-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Leaf promordia, trichomes, atrichoblasts,
CC fertilized eggs, seed coat.
CC -!- INDUCTION: Not induced by salicylic acid or wounding.
CC {ECO:0000269|PubMed:12084832}.
CC -!- SIMILARITY: Belongs to the WRKY group I family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC98047.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005896; AAC98047.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09373.1; -; Genomic_DNA.
DR EMBL; AF516172; AAM61951.1; -; mRNA.
DR EMBL; AF404862; AAK96200.1; -; mRNA.
DR PIR; E84790; E84790.
DR RefSeq; NP_001323504.1; NM_001336644.1.
DR RefSeq; NP_181263.2; NM_129282.4. [Q9ZUU0-1]
DR AlphaFoldDB; Q9ZUU0; -.
DR SMR; Q9ZUU0; -.
DR BioGRID; 3647; 7.
DR IntAct; Q9ZUU0; 3.
DR STRING; 3702.AT2G37260.1; -.
DR PaxDb; Q9ZUU0; -.
DR PRIDE; Q9ZUU0; -.
DR ProteomicsDB; 234414; -. [Q9ZUU0-1]
DR EnsemblPlants; AT2G37260.1; AT2G37260.1; AT2G37260. [Q9ZUU0-1]
DR GeneID; 818303; -.
DR Gramene; AT2G37260.1; AT2G37260.1; AT2G37260. [Q9ZUU0-1]
DR KEGG; ath:AT2G37260; -.
DR Araport; AT2G37260; -.
DR TAIR; locus:2049852; AT2G37260.
DR eggNOG; ENOG502QS6E; Eukaryota.
DR InParanoid; Q9ZUU0; -.
DR PhylomeDB; Q9ZUU0; -.
DR PRO; PR:Q9ZUU0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUU0; baseline and differential.
DR Genevisible; Q9ZUU0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009957; P:epidermal cell fate specification; IMP:TAIR.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR Gene3D; 2.20.25.80; -; 2.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31221; PTHR31221; 1.
DR Pfam; PF03106; WRKY; 2.
DR SMART; SM00774; WRKY; 2.
DR SUPFAM; SSF118290; SSF118290; 2.
DR PROSITE; PS50811; WRKY; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..429
FT /note="WRKY transcription factor 44"
FT /id="PRO_0000133685"
FT DNA_BIND 159..223
FT /note="WRKY 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT DNA_BIND 343..408
FT /note="WRKY 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 214..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 410..429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..279
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 292..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..333
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 190
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 195
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 218
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 374
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 379
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 403
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 405
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT MUTAGEN 398..429
FT /note="TYEGKHNHHLLLSPPSSSTLPFNSPQLSKQTI->SLQPTRVNIIIICS: In
FT ttg2-2; defects in trichome development, seed coat color
FT and mucilage production."
FT CONFLICT 417
FT /note="L -> F (in Ref. 3; AAM61951)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 429 AA; 47141 MW; DD5B0D55B7267612 CRC64;
MEVNDGERVV IAKPVASRPS SSSGFRTFTE LLTDSVTVSP QTTCHEIVDA AIRPKTLRFN
QPVAASVSCP RAEVKGIGNG MSCDDDSDSR NYVVYKPKAK LVSKATVSAL ANMLQGNRQQ
TWRQSEAVSY GKSVSQGTHR AGPNLVQKVP SFTESETSTG DRSSVDGYNW RKYGQKQVKG
SECPRSYYKC THPKCPVKKK VERSVEGQVS EIVYQGEHNH SKPSCPLPRR ASSSISSGFQ
KPPKSIASEG SMGQDPNNNL YSPLWNNQSN DSTQNRTEKM SEGCVITPFE FAVPRSTNSN
PGTSDSGCKS SQCDEGELDD PSRSKRRKNE KQSSEAGVSQ GSVESDSLED GFRWRKYGQK
VVGGNAYPRS YYRCTSANCR ARKHVERASD DPRAFITTYE GKHNHHLLLS PPSSSTLPFN
SPQLSKQTI
