WRK46_ARATH
ID WRK46_ARATH Reviewed; 295 AA.
AC Q9SKD9;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Probable WRKY transcription factor 46;
DE AltName: Full=WRKY DNA-binding protein 46;
GN Name=WRKY46; OrderedLocusNames=At2g46400; ORFNames=F11C10.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Ulker B., Somssich I.E.;
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INDUCTION BY SALICYLIC ACID.
RC STRAIN=cv. Columbia;
RX PubMed=22268143; DOI=10.1093/jxb/err450;
RA Besseau S., Li J., Palva E.T.;
RT "WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 63:2667-2679(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ERWINIA AMYLOVORA.
RC STRAIN=cv. Columbia;
RX PubMed=22316300; DOI=10.1094/mpmi-05-11-0111;
RA Moreau M., Degrave A., Vedel R., Bitton F., Patrit O., Renou J.-P.,
RA Barny M.-A., Fagard M.;
RT "EDS1 contributes to nonhost resistance of Arabidopsis thaliana against
RT Erwinia amylovora.";
RL Mol. Plant Microbe Interact. 25:421-430(2012).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY SALICYLIC ACID AND PSEUDOMONAS
RP SYRINGAE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=22325892; DOI=10.1016/j.plantsci.2011.12.003;
RA Hu Y., Dong Q., Yu D.;
RT "Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance
RT against pathogen Pseudomonas syringae.";
RL Plant Sci. 185:288-297(2012).
RN [8]
RP FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=24773321; DOI=10.1111/tpj.12538;
RA Ding Z.J., Yan J.Y., Xu X.Y., Yu D.Q., Li G.X., Zhang S.Q., Zheng S.J.;
RT "Transcription factor WRKY46 regulates osmotic stress responses and
RT stomatal movement independently in Arabidopsis.";
RL Plant J. 79:13-27(2014).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH BZR2/BES1, AND
RP PHOSPHORYLATION BY ASK7/BIN2.
RC STRAIN=cv. Columbia;
RX PubMed=28576847; DOI=10.1105/tpc.17.00364;
RA Chen J., Nolan T.M., Ye H., Zhang M., Tong H., Xin P., Chu J., Chu C.,
RA Li Z., Yin Y.;
RT "Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved
RT in brassinosteroid-regulated plant growth and drought responses.";
RL Plant Cell 29:1425-1439(2017).
CC -!- FUNCTION: Transcription factor involved in the regulation of osmotic
CC stress responses and stomatal movement (PubMed:24773321). Interacts
CC specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring
CC elicitor-responsive cis-acting element (By similarity). Positive
CC regulator of EDS1-dependent defense against E.amylovora
CC (PubMed:22316300). Together with WRKY70 and WRKY53, promotes resistance
CC to P.syringae, probably by enhancing salicylic acid (SA)- dependent
CC genes. Contributes to the suppression of jasmonic acid (MeJA)-induced
CC expression of PDF1.2 (PubMed:22325892). Together with WRKY54 and
CC WRKY70, promotes brassinosteroid (BR)-regulated plant growth but
CC prevent drought response by modulating gene expression
CC (PubMed:28576847). {ECO:0000250, ECO:0000269|PubMed:22316300,
CC ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321,
CC ECO:0000269|PubMed:28576847}.
CC -!- SUBUNIT: Binds to BZR2/BES1 to cooperatively regulate the expression of
CC target genes. {ECO:0000269|PubMed:28576847}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223}.
CC -!- TISSUE SPECIFICITY: Expressed in guard cells, hypocotyls, and in the
CC vascular tissues of cotyledon and root (PubMed:24773321). Mostly
CC expressed in roots, at lower levels in leaves and petioles, and, to a
CC lower extent, in stems, flowers and siliques (PubMed:22325892).
CC {ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321}.
CC -!- INDUCTION: Up-regulated by drought, salt and hydrogen peroxide
CC treatments (PubMed:24773321). Induced by salicylic acid (SA)
CC (PubMed:22268143, PubMed:22325892). Up-regulated by E.amylovora
CC (PubMed:22316300). Triggered by P.syringae (PubMed:22325892).
CC {ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300,
CC ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321}.
CC -!- PTM: Phosphorylated and destabilized by ASK7/BIN2.
CC {ECO:0000269|PubMed:28576847}.
CC -!- DISRUPTION PHENOTYPE: Decreased tolerance to dehydration and salt
CC stress (PubMed:24773321). Increase in the number of necrotic leaves and
CC in the intensity of necrosis in response to E.amylovora
CC (PubMed:22316300). Increased susceptibility to P.syringae associated
CC with reduced PR1 induction in double mutants wrky46 wrky70 and wrky46
CC wrky53, and triple mutant wrky46 wrky70 wrky53. In these mutants,
CC higher induction of PDF1.2 upon jasmonic acid (MeJA) treatment
CC (PubMed:22325892). The triple mutant wrky46 wrky54 wrky70 has defects
CC in brassinosteroid (BR)-regulated growth and is more tolerant to
CC drought stress (PubMed:28576847). {ECO:0000269|PubMed:22316300,
CC ECO:0000269|PubMed:22325892, ECO:0000269|PubMed:24773321,
CC ECO:0000269|PubMed:28576847}.
CC -!- SIMILARITY: Belongs to the WRKY group III family. {ECO:0000305}.
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DR EMBL; AY046275; AAK96020.1; -; mRNA.
DR EMBL; AC006526; AAD23042.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10690.1; -; Genomic_DNA.
DR EMBL; AY080812; AAL87292.1; -; mRNA.
DR EMBL; AY117210; AAM51285.1; -; mRNA.
DR PIR; D84902; D84902.
DR RefSeq; NP_182163.1; NM_130204.3.
DR AlphaFoldDB; Q9SKD9; -.
DR SMR; Q9SKD9; -.
DR BioGRID; 4583; 6.
DR IntAct; Q9SKD9; 4.
DR STRING; 3702.AT2G46400.1; -.
DR iPTMnet; Q9SKD9; -.
DR PaxDb; Q9SKD9; -.
DR PRIDE; Q9SKD9; -.
DR ProteomicsDB; 234289; -.
DR DNASU; 819248; -.
DR EnsemblPlants; AT2G46400.1; AT2G46400.1; AT2G46400.
DR GeneID; 819248; -.
DR Gramene; AT2G46400.1; AT2G46400.1; AT2G46400.
DR KEGG; ath:AT2G46400; -.
DR Araport; AT2G46400; -.
DR TAIR; locus:2039119; AT2G46400.
DR eggNOG; ENOG502R7UM; Eukaryota.
DR HOGENOM; CLU_058534_0_0_1; -.
DR InParanoid; Q9SKD9; -.
DR OMA; TCTQASH; -.
DR OrthoDB; 1331082at2759; -.
DR PhylomeDB; Q9SKD9; -.
DR PRO; PR:Q9SKD9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKD9; baseline and differential.
DR Genevisible; Q9SKD9; AT.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IPI:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:1900457; P:regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:2000070; P:regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR32096; PTHR32096; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; DNA-binding;
KW Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..295
FT /note="Probable WRKY transcription factor 46"
FT /id="PRO_0000133687"
FT DNA_BIND 98..166
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
SQ SEQUENCE 295 AA; 33635 MW; 116F5DF206AD854F CRC64;
MMMEEKLVIN ELELGKELAN RLMNNLKHTS SVDSNKTLIS DILRIYQNAI FMLSFNQDKN
ILKRSLEIDG KDSKNVFKKR KVSEKNTEKV KVFVATEQEN GSIDDGHCWR KYGQKEIHGS
KNPRAYYRCT HRFTQDCLAV KQVQKSDTDP SLFEVKYLGN HTCNNITSPK TTTNFSVSLT
NTNIFEGNRV HVTEQSEDMK PTKSEEVMIS LEDLENKKNI FRTFSFSNHE IENGVWKSNL
FLGNFVEDLS PATSGSAITS EVLSAPAAVE NSETADSYFS SLDNIIDFGQ DWLWS
