WRK46_HORVU
ID WRK46_HORVU Reviewed; 573 AA.
AC Q6VWJ6;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=WRKY transcription factor SUSIBA2 {ECO:0000305|PubMed:12953112};
DE AltName: Full=Sugar signaling in barley 2 {ECO:0000303|PubMed:12953112};
DE AltName: Full=WRKY transcription factor 46;
GN Name=WRKY46 {ECO:0000312|EMBL:AAQ63880.1};
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513 {ECO:0000312|EMBL:AAQ63880.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY
RP SUGAR, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Pongo;
RX PubMed=12953112; DOI=10.1105/tpc.014597;
RA Sun C., Palmqvist S., Olsson H., Boren M., Ahlandsberg S., Jansson C.;
RT "A novel WRKY transcription factor, SUSIBA2, participates in sugar
RT signaling in barley by binding to the sugar-responsive elements of the iso1
RT promoter.";
RL Plant Cell 15:2076-2092(2003).
RN [2]
RP FUNCTION.
RC STRAIN=cv. Pongo;
RX PubMed=16167901; DOI=10.1111/j.1365-313x.2005.02515.x;
RA Sun C., Hoeglund A.S., Olsson H., Mangelsen E., Jansson C.;
RT "Antisense oligodeoxynucleotide inhibition as a potent strategy in plant
RT biology: identification of SUSIBA2 as a transcriptional activator in plant
RT sugar signalling.";
RL Plant J. 44:128-138(2005).
RN [3]
RP BIOTECHNOLOGY.
RX PubMed=26200336; DOI=10.1038/nature14673;
RA Su J., Hu C., Yan X., Jin Y., Chen Z., Guan Q., Wang Y., Zhong D.,
RA Jansson C., Wang F., Schnuerer A., Sun C.;
RT "Expression of barley SUSIBA2 transcription factor yields high-starch low-
RT methane rice.";
RL Nature 523:602-606(2015).
CC -!- FUNCTION: Transcription factor involved in starch synthesis
CC (PubMed:12953112). Acts as a transcriptional activator in sugar
CC signaling (PubMed:16167901). Interacts specifically with the SURE and
CC W-box elements, but not with the SP8a element (PubMed:12953112).
CC {ECO:0000269|PubMed:12953112, ECO:0000269|PubMed:16167901}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12953112}.
CC -!- TISSUE SPECIFICITY: Expressed in endosperm, but not in leaves.
CC {ECO:0000269|PubMed:12953112}.
CC -!- DEVELOPMENTAL STAGE: Peak of expression 12 days after pollination.
CC {ECO:0000269|PubMed:12953112}.
CC -!- INDUCTION: Up-regulated by sugar. {ECO:0000269|PubMed:12953112}.
CC -!- BIOTECHNOLOGY: Expression of barley SUSIBA2 transcription factor in
CC rice yields plants with increased starch content and reduced methane
CC emission. {ECO:0000269|PubMed:26200336}.
CC -!- SIMILARITY: Belongs to the WRKY group I family. {ECO:0000305}.
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DR EMBL; AY323206; AAQ63880.1; -; mRNA.
DR AlphaFoldDB; Q6VWJ6; -.
DR SMR; Q6VWJ6; -.
DR ExpressionAtlas; Q6VWJ6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IEA:EnsemblPlants.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR GO; GO:0009961; P:response to 1-aminocyclopropane-1-carboxylic acid; IEA:EnsemblPlants.
DR GO; GO:0009611; P:response to wounding; IEA:EnsemblPlants.
DR Gene3D; 2.20.25.80; -; 2.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31221; PTHR31221; 1.
DR Pfam; PF03106; WRKY; 2.
DR SMART; SM00774; WRKY; 2.
DR SUPFAM; SSF118290; SSF118290; 2.
DR PROSITE; PS50811; WRKY; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Repeat; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..573
FT /note="WRKY transcription factor SUSIBA2"
FT /id="PRO_0000434099"
FT DNA_BIND 183..247
FT /note="WRKY 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT DNA_BIND 357..422
FT /note="WRKY 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 56..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 240..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..83
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 316..332
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 242
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 244
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 388
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 393
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 417
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
FT BINDING 419
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9SI37"
SQ SEQUENCE 573 AA; 62243 MW; B21B894C29C0DC64 CRC64;
MSPARLPISR ESCLTIPAGF SPSALLDSPV LLTNFKVEPS PTTGSLGMAA ILHKSAHPDM
LPSPRDKSVR NAHEDRGSRD FEFKPHLNSS SQSLAPAMSD LKKHEHSMQN QSMNPSSSSS
NMVNENRPPC SRESSLTVNV SAQNQPVGMV GLTDSMPAEV GTSEPQQMNS SDNAMQEPQS
ENVADKSADD GYNWRKYGQK HVKGSENPRS YYKCTHPNCE VKKLLERAVD GLITEVVYKG
RHNHPKPQPN RRLAGGAVPS NQGEERYDGA SAADDKSSNA LSNLANPVHS PGMVEPVPAS
VSDDDIDAGG GRPYPGDDAT EEEDLESKRR KMESAGIDAA LMGKPNREPR VVVQTVSEVD
ILDDGYRWRK YGQKVVKGNP NPRSYYKCTS TGCPVRKHVE RASHDPKSVI TTYEGKHNHE
VPAARNATHE MSAPPMKNVV HQINSNMPSS IGGMMRACEA RNYTNQYSQA AETDTVSLDL
GVGISPNHSD ATNQMQSSGP DQMQYQMQTM GSMYGNMRHP SSMAAPAVQG NSAARMYGSR
EEKGNEGFTF RATPMDHSAN LCYSSAGNLV MGP
