WRK54_ARATH
ID WRK54_ARATH Reviewed; 346 AA.
AC Q93WU8;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable WRKY transcription factor 54 {ECO:0000303|PubMed:22268143};
DE AltName: Full=WRKY DNA-binding protein 54 {ECO:0000303|PubMed:22268143};
GN Name=WRKY54 {ECO:0000303|PubMed:22268143};
GN OrderedLocusNames=At2g40750 {ECO:0000312|Araport:AT2G40750};
GN ORFNames=T7D17.7 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Kushnir S., Ulker B., Somssich I.E.;
RL Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INTERACTION WITH WRKY30, AND INDUCTION BY REACTIVE OXYGEN SPECIES AND
RP SALICYLIC ACID.
RC STRAIN=cv. Columbia;
RX PubMed=22268143; DOI=10.1093/jxb/err450;
RA Besseau S., Li J., Palva E.T.;
RT "WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 63:2667-2679(2012).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ERWINIA AMYLOVORA.
RC STRAIN=cv. Columbia;
RX PubMed=22316300; DOI=10.1094/mpmi-05-11-0111;
RA Moreau M., Degrave A., Vedel R., Bitton F., Patrit O., Renou J.-P.,
RA Barny M.-A., Fagard M.;
RT "EDS1 contributes to nonhost resistance of Arabidopsis thaliana against
RT Erwinia amylovora.";
RL Mol. Plant Microbe Interact. 25:421-430(2012).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY OSMOTIC STRESS.
RC STRAIN=cv. Columbia;
RX PubMed=23815736; DOI=10.1111/nph.12378;
RA Li J., Besseau S., Toeroenen P., Sipari N., Kollist H., Holm L.,
RA Palva E.T.;
RT "Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic
RT stress tolerance by regulating stomatal aperture in Arabidopsis.";
RL New Phytol. 200:457-472(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH BZR2/BES1, AND
RP PHOSPHORYLATION BY ASK7/BIN2.
RC STRAIN=cv. Columbia;
RX PubMed=28576847; DOI=10.1105/tpc.17.00364;
RA Chen J., Nolan T.M., Ye H., Zhang M., Tong H., Xin P., Chu J., Chu C.,
RA Li Z., Yin Y.;
RT "Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved
RT in brassinosteroid-regulated plant growth and drought responses.";
RL Plant Cell 29:1425-1439(2017).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=28837631; DOI=10.1371/journal.pone.0183731;
RA Li J., Zhong R., Palva E.T.;
RT "WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated
RT defenses to necrotrophic pathogens in Arabidopsis.";
RL PLoS ONE 12:E0183731-E0183731(2017).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element (By similarity). Together with WRKY70, negative
CC regulator of developmental senescence, probably via the regulation of
CC several senescence-associated markers genes (PubMed:22268143). Positive
CC regulator of EDS1-dependent defense against E.amylovora
CC (PubMed:22316300). In collaboration with WRKY70, prevents stomatal
CC closure and, consequently, osmotic stress tolerance (PubMed:23815736).
CC Together with WRKY46 and WRKY70, promotes brassinosteroid (BR)-
CC regulated plant growth but prevent drought response by modulating gene
CC expression (PubMed:28576847). Negative regulator of SA biosynthesis
CC (PubMed:28837631). Prevents defense response to the necrotrophic
CC pathogens P.carotovorum and B.cinerea, but promotes defense against
CC biotrophic/hemibiotrophic pathogens P.syringae pv. tomato (Pst) DC3000,
CC probably by regulating negatively the jasmonic acid (JA)/ethylene (ET)
CC and positively the salicylic acid (SA) signaling pathways
CC (PubMed:28837631). {ECO:0000250|UniProtKB:Q9SUP6,
CC ECO:0000269|PubMed:22268143, ECO:0000269|PubMed:22316300,
CC ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:28576847,
CC ECO:0000269|PubMed:28837631, ECO:0000303|PubMed:28837631}.
CC -!- SUBUNIT: Interacts with WRKY30 (PubMed:22268143). Binds to BZR2/BES1 to
CC cooperatively regulate the expression of target genes. Interacts with
CC ASK7/BIN2 (PubMed:28576847). {ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:28576847}.
CC -!- INTERACTION:
CC Q93WU8; Q9SZI2: NAP1;1; NbExp=3; IntAct=EBI-15207592, EBI-4424361;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:22268143}.
CC -!- DEVELOPMENTAL STAGE: In leaves, level increases gradually up to the
CC point of leaf senescence. {ECO:0000269|PubMed:22268143}.
CC -!- INDUCTION: Induced by reactive oxygen species (ROS) and salicylic acid
CC (SA) (PubMed:22268143). Early but transient accumulation after osmotic
CC stress (e.g. polyethylene glycol, PEG) (PubMed:23815736). Up-regulated
CC by E.amylovora (PubMed:22316300). {ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:23815736}.
CC -!- PTM: Phosphorylated and destabilized by ASK7/BIN2.
CC {ECO:0000269|PubMed:28576847}.
CC -!- DISRUPTION PHENOTYPE: Promotion of leaf senescence associated with
CC abnormal expression levels of several senescence-associated markers
CC genes. The double mutant wrky54 wrky70 exhibits stronger leaf
CC senescence symptoms (PubMed:22268143). Increase in the number of
CC necrotic leaves and in the intensity of necrosis in response to
CC E.amylovora (PubMed:22316300). The double mutant wrky54 wrky70 exhibits
CC an enhanced tolerance to osmotic stress associated with improved water
CC retention and enhanced stomatal closure as well as salicylic acid (SA)
CC accumulation, but a reduced induction of osmotic stress-responsive
CC genes and reduced accumulation of the osmoprotectant proline
CC (PubMed:23815736). Unstressed wrky54 wrky70 double mutant exhibits
CC increased levels of SA, moderate accumulation of hydrogen peroxide
CC H(2)O(2) and up-regulated expression of both SA and JA/ethylene (ET)
CC responsive defense related genes; thus promoting cell wall
CC fortification and consequently enhancing resistance to necrotrophic
CC pathogens (e.g. P.carotovorum and B.cinerea) associated with reduced
CC cell death, but is not sufficient to trigger hypersensitive reaction
CC (HR)-like cell death and resistance to biotrophic/hemibiotrophic
CC pathogens (e.g. P.syringae), characterized by reduced amount of callose
CC (PubMed:28837631). The triple mutant wrky46 wrky54 wrky70 has defects
CC in brassinosteroid (BR)-regulated growth and is more tolerant to
CC drought stress (PubMed:28576847). {ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:23815736,
CC ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631}.
CC -!- SIMILARITY: Belongs to the WRKY group III family. {ECO:0000305}.
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DR EMBL; AF426253; AAL29430.1; -; mRNA.
DR EMBL; CP002685; AEC09872.1; -; Genomic_DNA.
DR RefSeq; NP_181607.1; NM_129637.3.
DR AlphaFoldDB; Q93WU8; -.
DR SMR; Q93WU8; -.
DR BioGRID; 4008; 5.
DR IntAct; Q93WU8; 4.
DR STRING; 3702.AT2G40750.1; -.
DR PaxDb; Q93WU8; -.
DR PRIDE; Q93WU8; -.
DR EnsemblPlants; AT2G40750.1; AT2G40750.1; AT2G40750.
DR GeneID; 818670; -.
DR Gramene; AT2G40750.1; AT2G40750.1; AT2G40750.
DR KEGG; ath:AT2G40750; -.
DR Araport; AT2G40750; -.
DR TAIR; locus:2064806; AT2G40750.
DR eggNOG; ENOG502RYCZ; Eukaryota.
DR HOGENOM; CLU_066547_0_0_1; -.
DR InParanoid; Q93WU8; -.
DR OMA; YERTSAM; -.
DR OrthoDB; 891144at2759; -.
DR PRO; PR:Q93WU8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q93WU8; baseline and differential.
DR Genevisible; Q93WU8; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:1900056; P:negative regulation of leaf senescence; IGI:TAIR.
DR GO; GO:1900457; P:regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0031347; P:regulation of defense response; IMP:TAIR.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0047484; P:regulation of response to osmotic stress; IMP:UniProtKB.
DR GO; GO:2000070; P:regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0090333; P:regulation of stomatal closure; IMP:UniProtKB.
DR GO; GO:0006970; P:response to osmotic stress; IEP:UniProtKB.
DR GO; GO:0000302; P:response to reactive oxygen species; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:TAIR.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR32096; PTHR32096; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; DNA-binding; Ethylene signaling pathway;
KW Jasmonic acid signaling pathway; Nucleus; Phosphoprotein; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..346
FT /note="Probable WRKY transcription factor 54"
FT /id="PRO_0000133695"
FT DNA_BIND 146..214
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 109..130
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 143
FT /note="T -> A (in Ref. 1; AAL29430)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="F -> L (in Ref. 1; AAL29430)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 346 AA; 38645 MW; B1EEB2DE80AA5A8D CRC64;
MDSNSNNTKS IKRKVVDQLV EGYEFATQLQ LLLSHQHSNQ YHIDETRLVS GSGSVSGGPD
PVDELMSKIL GSFHKTISVL DSFDPVAVSV PIAVEGSWNA SCGDDSATPV SCNGGDSGES
KKKRLGVGKG KRGCYTRKTR SHTRIVEAKS SEDRYAWRKY GQKEILNTTF PRSYFRCTHK
PTQGCKATKQ VQKQDQDSEM FQITYIGYHT CTANDQTHAK TEPFDQEIIM DSEKTLAAST
AQNHVNAMVQ EQENNTSSVT AIDAGMVKEE QNNNGDQSKD YYEGSSTGED LSLVWQETMM
FDDHQNHYYC GETSTTSHQF GFIDNDDQFS SFFDSYCADY ERTSAM
