WRK62_ORYSJ
ID WRK62_ORYSJ Reviewed; 318 AA.
AC Q6EPZ0; Q20DP8;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=WRKY transcription factor WRKY62 {ECO:0000303|PubMed:16528562};
DE Short=OsWRKY62 {ECO:0000303|PubMed:16528562};
DE AltName: Full=XA21-binding protein 10 {ECO:0000305};
GN Name=WRKY62 {ECO:0000303|PubMed:16528562}; Synonyms=XB10 {ECO:0000305};
GN OrderedLocusNames=Os09g0417800 {ECO:0000312|EMBL:BAF25099.1};
GN ORFNames=OsJ_29379 {ECO:0000312|EMBL:EEE69711.1},
GN OSNPB_090417800 {ECO:0000312|EMBL:BAT08100.1},
GN P0014G10.42 {ECO:0000312|EMBL:BAD29280.1},
GN P0701F11.1 {ECO:0000312|EMBL:BAD28643.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INDUCTION BY SALICYLIC ACID;
RP MAGNAPORTHE GRISEA AND XANTHOMONAS ORYZAE.
RX PubMed=16528562; DOI=10.1007/s00299-006-0138-1;
RA Ryu H.-S., Han M., Lee S.-K., Cho J.-I., Ryoo N., Heu S., Lee Y.-H.,
RA Bhoo S.H., Wang G.-L., Hahn T.-R., Jeon J.-S.;
RT "A comprehensive expression analysis of the WRKY gene superfamily in rice
RT plants during defense response.";
RL Plant Cell Rep. 25:836-847(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=15629062; DOI=10.1186/1471-2148-5-1;
RA Zhang Y., Wang L.;
RT "The WRKY transcription factor superfamily: its origin in eukaryotes and
RT expansion in plants.";
RL BMC Evol. Biol. 5:1-1(2005).
RN [8]
RP GENE FAMILY.
RX PubMed=16106749; DOI=10.1093/dnares/12.1.9;
RA Wu K.-L., Guo Z.-J., Wang H.-H., Li J.;
RT "The WRKY family of transcription factors in rice and Arabidopsis and their
RT origins.";
RL DNA Res. 12:9-26(2005).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [10]
RP INDUCTION BY BENZOTHIADIAZOLE AND SALICYLIC ACID.
RX PubMed=17601827; DOI=10.1105/tpc.106.046250;
RA Shimono M., Sugano S., Nakayama A., Jiang C.-J., Ono K., Toki S.,
RA Takatsuji H.;
RT "Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast
RT resistance.";
RL Plant Cell 19:2064-2076(2007).
RN [11]
RP ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION,
RP AND INTERACTION WITH XA21.
RX PubMed=19825552; DOI=10.1093/mp/ssn024;
RA Peng Y., Bartley L.E., Chen X., Dardick C., Chern M., Ruan R., Canlas P.E.,
RA Ronald P.C.;
RT "OsWRKY62 is a negative regulator of basal and Xa21-mediated defense
RT against Xanthomonas oryzae pv. oryzae in rice.";
RL Mol. Plant 1:446-458(2008).
RN [12]
RP FUNCTION.
RX PubMed=21961049; DOI=10.1007/s12284-010-9039-6;
RA Peng Y., Bartley L.E., Canlas P., Ronald P.C.;
RT "OsWRKY IIa transcription factors modulate rice innate immunity.";
RL Rice 3:36-42(2010).
RN [13]
RP INTERACTION WITH XA21, SUBCELLULAR LOCATION, AND INDUCTION BY XA21.
RX PubMed=22735448; DOI=10.1038/ncomms1932;
RA Park C.J., Ronald P.C.;
RT "Cleavage and nuclear localization of the rice XA21 immune receptor.";
RL Nat. Commun. 3:920-920(2012).
RN [14]
RP INDUCTION BY WRKY45; BENZOTHIADIAZOLE AND MAGNAPORTHE GRISEA.
RX PubMed=24093634; DOI=10.1186/1471-2229-13-150;
RA Nakayama A., Fukushima S., Goto S., Matsushita A., Shimono M., Sugano S.,
RA Jiang C.J., Akagi A., Yamazaki M., Inoue H., Takatsuji H.;
RT "Genome-wide identification of WRKY45-regulated genes that mediate
RT benzothiadiazole-induced defense responses in rice.";
RL BMC Plant Biol. 13:150-150(2013).
RN [15]
RP INDUCTION BY BIOTIC ELICITORS.
RC STRAIN=cv. Nipponbare;
RX PubMed=23462973; DOI=10.1007/s11103-013-0032-5;
RA Chujo T., Miyamoto K., Shimogawa T., Shimizu T., Otake Y., Yokotani N.,
RA Nishizawa Y., Shibuya N., Nojiri H., Yamane H., Minami E., Okada K.;
RT "OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate
RT immune responses in rice against rice blast fungus.";
RL Plant Mol. Biol. 82:23-37(2013).
CC -!- FUNCTION: Transcription repressor. Interacts specifically with the W
CC box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive
CC cis-acting element. Regulates, probably indirectly, the activation of
CC defense-related genes during defense response (By similarity).
CC Regulates negatively basal innate immunity and XA21-mediated defense
CC against X.oryzae pv. oryzae (Xoo) (PubMed:19825552, PubMed:21961049).
CC {ECO:0000250|UniProtKB:Q6QHD1, ECO:0000269|PubMed:19825552,
CC ECO:0000269|PubMed:21961049}.
CC -!- SUBUNIT: Interacts with XA21 in the nucleus.
CC {ECO:0000269|PubMed:19825552, ECO:0000269|PubMed:22735448}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:19825552, ECO:0000269|PubMed:22735448}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=OsWRKY62.1 {ECO:0000303|PubMed:19825552};
CC IsoId=Q6EPZ0-1; Sequence=Displayed;
CC Name=2; Synonyms=OsWRKY62.2 {ECO:0000303|PubMed:19825552};
CC IsoId=Q6EPZ0-2; Sequence=VSP_058456;
CC -!- INDUCTION: Might be activated by XA21 in the nucleus upon pathogen
CC infection (PubMed:19825552). Induced by biotic elicitors (e.g. fungal
CC chitin oligosaccharide) (PubMed:23462973). Induced by pathogen
CC infection (e.g. M.grisea and X.oryzae pv. oryzae (Xoo))
CC (PubMed:16528562, PubMed:24093634). Accumulates after treatment with
CC benzothiadiazole (BTH) and salicylic acid (SA) (PubMed:17601827,
CC PubMed:16528562, PubMed:24093634). Transactivated by WRKY45
CC (PubMed:24093634). {ECO:0000269|PubMed:16528562,
CC ECO:0000269|PubMed:17601827, ECO:0000269|PubMed:23462973,
CC ECO:0000269|PubMed:24093634, ECO:0000303|PubMed:19825552}.
CC -!- DOMAIN: The WRKY domain is required to bind DNA.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
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DR EMBL; DQ298182; ABC02810.1; -; mRNA.
DR EMBL; AP005429; BAD28643.1; -; Genomic_DNA.
DR EMBL; AP005784; BAD29280.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25099.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT08100.1; -; Genomic_DNA.
DR EMBL; CM000146; EEE69711.1; -; Genomic_DNA.
DR EMBL; AK067834; BAG90631.1; -; mRNA.
DR RefSeq; XP_015610755.1; XM_015755269.1. [Q6EPZ0-1]
DR AlphaFoldDB; Q6EPZ0; -.
DR SMR; Q6EPZ0; -.
DR STRING; 4530.OS09T0417800-01; -.
DR PaxDb; Q6EPZ0; -.
DR PRIDE; Q6EPZ0; -.
DR EnsemblPlants; Os09t0417800-01; Os09t0417800-01; Os09g0417800. [Q6EPZ0-1]
DR EnsemblPlants; Os09t0417800-02; Os09t0417800-02; Os09g0417800. [Q6EPZ0-2]
DR GeneID; 4347070; -.
DR Gramene; Os09t0417800-01; Os09t0417800-01; Os09g0417800. [Q6EPZ0-1]
DR Gramene; Os09t0417800-02; Os09t0417800-02; Os09g0417800. [Q6EPZ0-2]
DR KEGG; osa:4347070; -.
DR eggNOG; ENOG502QR7M; Eukaryota.
DR HOGENOM; CLU_047067_0_1_1; -.
DR InParanoid; Q6EPZ0; -.
DR OMA; FMHALTY; -.
DR OrthoDB; 1185192at2759; -.
DR PlantReactome; R-OSA-6788019; Salicylic acid signaling.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000007752; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:1900425; P:negative regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0010200; P:response to chitin; IEP:UniProtKB.
DR GO; GO:0009620; P:response to fungus; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Coiled coil; DNA-binding;
KW Nucleotide-binding; Nucleus; Plant defense; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..318
FT /note="WRKY transcription factor WRKY62"
FT /id="PRO_0000436956"
FT DNA_BIND 138..204
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 29..49
FT /evidence="ECO:0000255"
FT BINDING 259..266
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00289"
FT VAR_SEQ 1..39
FT /note="Missing (in isoform 2)"
FT /id="VSP_058456"
SQ SEQUENCE 318 AA; 33515 MW; D5C96CAE89B5637C CRC64;
MDDDGDGSSS PTDDSAAAGL LPLFSRSPAE DLEEKLRRAM EENARLTRAL DAILAGHHAH
QRALLAPSLS PPPPSATARA PSVSTSCAAR EDAAPAVAAA AASTACPSRQ QPPTAEPRPK
VRTVRVRADA ADATDANSMA ETVKDGYQWR KYGQKVTRDN PYPRAYFRCA FAPSCPVKKK
LQRCAEDRSM LVATYEGEHN HALSTQTTEF VASGCTTSQH AGGSSSSPLP CSISINSSGR
TITLDLTNQA GSGSIASCGV EAAAVSGELV TVLSPELRRH LVEEVVQVLK NDAEFVEAVT
NAVAARVVDQ IPHIPVHL
