WRK70_ARATH
ID WRK70_ARATH Reviewed; 294 AA.
AC Q9LY00; Q8LB71;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Probable WRKY transcription factor 70 {ECO:0000303|Ref.1};
DE AltName: Full=WRKY DNA-binding protein 70 {ECO:0000303|Ref.1};
GN Name=WRKY70 {ECO:0000303|Ref.1};
GN OrderedLocusNames=At3g56400 {ECO:0000312|Araport:AT3G56400};
GN ORFNames=T5P19.50 {ECO:0000312|EMBL:CAB88043.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower;
RA Ulker B., Kushnir S., Somssich I.E.;
RT "Arabidopsis thaliana transcription factor WRKY70.";
RL Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ERWINIA CAROTOVORA;
RP SALICYLIC ACID AND JASMONIC ACID.
RX PubMed=14742872; DOI=10.1105/tpc.016980;
RA Li J., Brader G., Palva E.T.;
RT "The WRKY70 transcription factor: a node of convergence for jasmonate-
RT mediated and salicylate-mediated signals in plant defense.";
RL Plant Cell 16:319-331(2004).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16623907; DOI=10.1111/j.1365-313x.2006.02712.x;
RA Li J., Brader G., Kariola T., Palva E.T.;
RT "WRKY70 modulates the selection of signaling pathways in plant defense.";
RL Plant J. 46:477-491(2006).
RN [8]
RP INDUCTION BY PSEUDOMONAS SYRINGAE AND ATX1.
RX PubMed=17965588; DOI=10.4161/epi.2.2.4404;
RA Alvarez-Venegas R., Abdallat A.A., Guo M., Alfano J.R., Avramova Z.;
RT "Epigenetic control of a transcription factor at the cross section of two
RT antagonistic pathways.";
RL Epigenetics 2:106-113(2007).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=17313163; DOI=10.1094/mpmi-20-2-0120;
RA Knoth C., Ringler J., Dangl J.L., Eulgem T.;
RT "Arabidopsis WRKY70 is required for full RPP4-mediated disease resistance
RT and basal defense against Hyaloperonospora parasitica.";
RL Mol. Plant Microbe Interact. 20:120-128(2007).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION BY
RP SENESCENCE, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=17310369; DOI=10.1007/s00425-006-0474-y;
RA Uelker B., Shahid Mukhtar M., Somssich I.E.;
RT "The WRKY70 transcription factor of Arabidopsis influences both the plant
RT senescence and defense signaling pathways.";
RL Planta 226:125-137(2007).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, AND REPRESSION BY JASMONIC ACID.
RX PubMed=18713432; DOI=10.1111/j.1744-7909.2008.00653.x;
RA Ren C.-M., Zhu Q., Gao B.-D., Ke S.-Y., Yu W.-C., Xie D.-X., Peng W.;
RT "Transcription factor WRKY70 displays important but no indispensable roles
RT in jasmonate and salicylic acid signaling.";
RL J. Integr. Plant Biol. 50:630-637(2008).
RN [12]
RP REGULATION BY ATX1.
RC STRAIN=cv. Columbia;
RX PubMed=18375658; DOI=10.1105/tpc.107.056614;
RA Saleh A., Alvarez-Venegas R., Yilmaz M., Le O., Hou G., Sadder M.,
RA Al-Abdallat A., Xia Y., Lu G., Ladunga I., Avramova Z.;
RT "The highly similar Arabidopsis homologs of trithorax ATX1 and ATX2 encode
RT proteins with divergent biochemical functions.";
RL Plant Cell 20:568-579(2008).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INTERACTION WITH WRKY30, AND INDUCTION BY REACTIVE OXYGEN SPECIES AND
RP SALICYLIC ACID.
RC STRAIN=cv. Columbia;
RX PubMed=22268143; DOI=10.1093/jxb/err450;
RA Besseau S., Li J., Palva E.T.;
RT "WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 63:2667-2679(2012).
RN [14]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY ERWINIA AMYLOVORA.
RC STRAIN=cv. Columbia;
RX PubMed=22316300; DOI=10.1094/mpmi-05-11-0111;
RA Moreau M., Degrave A., Vedel R., Bitton F., Patrit O., Renou J.-P.,
RA Barny M.-A., Fagard M.;
RT "EDS1 contributes to nonhost resistance of Arabidopsis thaliana against
RT Erwinia amylovora.";
RL Mol. Plant Microbe Interact. 25:421-430(2012).
RN [15]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY SALICYLIC ACID AND
RP PSEUDOMONAS SYRINGAE.
RC STRAIN=cv. Columbia;
RX PubMed=22325892; DOI=10.1016/j.plantsci.2011.12.003;
RA Hu Y., Dong Q., Yu D.;
RT "Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance
RT against pathogen Pseudomonas syringae.";
RL Plant Sci. 185:288-297(2012).
RN [16]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY OSMOTIC STRESS.
RC STRAIN=cv. Columbia;
RX PubMed=23815736; DOI=10.1111/nph.12378;
RA Li J., Besseau S., Toeroenen P., Sipari N., Kollist H., Holm L.,
RA Palva E.T.;
RT "Defense-related transcription factors WRKY70 and WRKY54 modulate osmotic
RT stress tolerance by regulating stomatal aperture in Arabidopsis.";
RL New Phytol. 200:457-472(2013).
RN [17]
RP INDUCTION BY MYB44.
RC STRAIN=cv. Columbia;
RX PubMed=23067202; DOI=10.1111/tpj.12051;
RA Shim J.S., Jung C., Lee S., Min K., Lee Y.-W., Choi Y., Lee J.S.,
RA Song J.T., Kim J.-K., Choi Y.D.;
RT "AtMYB44 regulates WRKY70 expression and modulates antagonistic interaction
RT between salicylic acid and jasmonic acid signaling.";
RL Plant J. 73:483-495(2013).
RN [18]
RP INDUCTION BY MYB44.
RX PubMed=23603962; DOI=10.4161/psb.24509;
RA Shim J.S., Choi Y.D.;
RT "Direct regulation of WRKY70 by AtMYB44 in plant defense responses.";
RL Plant Signal. Behav. 8:E20783-E20783(2013).
RN [19]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=24104863; DOI=10.1007/s11103-013-0136-y;
RA Machens F., Becker M., Umrath F., Hehl R.;
RT "Identification of a novel type of WRKY transcription factor binding site
RT in elicitor-responsive cis-sequences from Arabidopsis thaliana.";
RL Plant Mol. Biol. 84:371-385(2014).
RN [20]
RP INDUCTION BY PLUTELLA XYLOSTELLA AND BREVICORYNE BRASSICAE.
RC STRAIN=cv. Columbia;
RX PubMed=25339349; DOI=10.1093/pcp/pcu150;
RA Kroes A., van Loon J.J.A., Dicke M.;
RT "Density-dependent interference of aphids with caterpillar-induced defenses
RT in Arabidopsis: involvement of phytohormones and transcription factors.";
RL Plant Cell Physiol. 56:98-106(2015).
RN [21]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY BACILLUS CEREUS; SALICYLIC
RP ACID AND BTH, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=26433201; DOI=10.1093/jxb/erv445;
RA Jiang C.-H., Huang Z.-Y., Xie P., Gu C., Li K., Wang D.-C., Yu Y.-Y.,
RA Fan Z.-H., Wang C.-J., Wang Y.-P., Guo Y.-H., Guo J.-H.;
RT "Transcription factors WRKY70 and WRKY11 served as regulators in
RT rhizobacterium Bacillus cereus AR156-induced systemic resistance to
RT Pseudomonas syringae pv. tomato DC3000 in Arabidopsis.";
RL J. Exp. Bot. 67:157-174(2016).
RN [22]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH BZR2/BES1, AND
RP PHOSPHORYLATION BY ASK7/BIN2.
RC STRAIN=cv. Columbia;
RX PubMed=28576847; DOI=10.1105/tpc.17.00364;
RA Chen J., Nolan T.M., Ye H., Zhang M., Tong H., Xin P., Chu J., Chu C.,
RA Li Z., Yin Y.;
RT "Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved
RT in brassinosteroid-regulated plant growth and drought responses.";
RL Plant Cell 29:1425-1439(2017).
RN [23]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=28837631; DOI=10.1371/journal.pone.0183731;
RA Li J., Zhong R., Palva E.T.;
RT "WRKY70 and its homolog WRKY54 negatively modulate the cell wall-associated
RT defenses to necrotrophic pathogens in Arabidopsis.";
RL PLoS ONE 12:E0183731-E0183731(2017).
CC -!- FUNCTION: Transcription factor involved in senescence, biotic and
CC abiotic stress responses by modulating various phytohormones signaling
CC pathways (PubMed:14742872, PubMed:16623907, PubMed:17310369,
CC PubMed:28576847). Interacts specifically with the W box (5'-
CC (T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting
CC element (By similarity). Binds to the 5'-[CT]GACTTTT-3' motif in
CC promoters of target genes to induce their expression (PubMed:24104863).
CC Plays an important but not indispensable role in jasmonate and
CC salicylic acid signaling (PubMed:18713432). Regulates positively the
CC salicylic acid (SA)-mediated signal pathway, but negatively the
CC jasmonic acid (JA)-mediated signal pathway, thus determining the
CC balance between these mutually antagonistic pathways (PubMed:14742872,
CC PubMed:16623907, PubMed:18713432, PubMed:28837631). Together with
CC WRKY46, WRKY53 and WRKY54, prevents defense response to the
CC necrotrophic pathogens P.carotovorum and B.cinerea, but promotes
CC defense responses (including SA-induced pathogenesis-related (PR) genes
CC expression) against biotrophic/hemibiotrophic SA-monitored pathogens
CC (e.g. P.syringae, E.carotovora subsp. carotovora SCC3193 and
CC E.cichoracearum), probably by regulating negatively the JA/ET and
CC positively the SA signaling pathways (PubMed:28837631, PubMed:16623907,
CC PubMed:22325892). Contributes to the suppression of jasmonic acid
CC (MeJA)-induced expression of JA-responsive genes (e.g. PDF1.2)
CC (PubMed:22325892, PubMed:16623907). Promotes susceptibility to JA-
CC monitored pathogens (e.g. A.brassicicola), probably by facilitating SA-
CC controlled suppression of JA-mediated defense. Represses the
CC biosynthesis of the phytoalexin camalexin and indol-3-ylmethyl
CC glucosinolate (IGS) (PubMed:16623907). Represses both SA and
CC JA/ethylene (ET) mediated defense marker genes expression
CC (PubMed:17310369). Negative regulator of SA biosynthesis
CC (PubMed:28837631). Negative regulator of EDS1-dependent defense against
CC E.amylovora (PubMed:22316300). Required for RPP4-mediated disease
CC resistance and basal defense against H.parasitica, probably via late
CC up-regulation (LURP) of resistance genes (e.g. CML10/CaBP22 and LURP1)
CC (PubMed:17313163). Probably involved in defense responses toward
CC insects (e.g. P.xylostella and B.brassicae) (PubMed:25339349). Together
CC with WRKY54, negative regulator of developmental senescence, probably
CC via the regulation of several senescence-associated markers genes
CC (PubMed:17310369, PubMed:22268143). Together with WRKY46 and WRKY54,
CC promotes brassinosteroid (BR)-regulated plant growth but prevent
CC drought response by modulating gene expression (PubMed:28576847). In
CC collaboration with WRKY54, prevents stomatal closure and, consequently,
CC osmotic stress tolerance (PubMed:23815736). Regulates rhizobacterium
CC B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv.
CC tomato DC3000 (PubMed:26433201). {ECO:0000250|UniProtKB:Q9SUP6,
CC ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:16623907,
CC ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17313163,
CC ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892,
CC ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:24104863,
CC ECO:0000269|PubMed:25339349, ECO:0000269|PubMed:26433201,
CC ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631,
CC ECO:0000303|PubMed:28837631}.
CC -!- SUBUNIT: Interacts with WRKY30 (PubMed:22268143). Binds to BZR2/BES1 to
CC cooperatively regulate the expression of target genes
CC (PubMed:28576847). {ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:28576847}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000255|PROSITE-ProRule:PRU00768, ECO:0000269|PubMed:17310369,
CC ECO:0000269|PubMed:26433201}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and flowers.
CC {ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:22268143}.
CC -!- DEVELOPMENTAL STAGE: In flowers, first observed in both stigmatic
CC papillae and the flower abscission zone, later confined to the
CC abscission zone (PubMed:17310369). In leaves, level increases gradually
CC up to the point of leaf senescence (PubMed:17310369, PubMed:22268143).
CC {ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:22268143}.
CC -!- INDUCTION: Regulated by MYB44 (PubMed:23067202, PubMed:23603962). Basal
CC expression levels require the presence of endogeneous salicylic acid
CC (SA) (PubMed:14742872, PubMed:17310369). Induced by reactive oxygen
CC species (ROS) (PubMed:22268143). Early but transient accumulation after
CC osmotic stress (e.g. polyethylene glycol, PEG) (PubMed:23815736).
CC Induced by SA; early induction is NPR1-independent, but full-scale
CC induction is NPR1-dependent (PubMed:14742872, PubMed:22325892,
CC PubMed:22268143, PubMed:26433201). Up-regulated by benzothiadiazole
CC (BTH) (PubMed:26433201). Repressed by jasmonic acid (MeJA) by both
CC COI1-dependent and COI1-independent pathways (PubMed:14742872,
CC PubMed:18713432). Triggered by the pathogenic compatible bacteria
CC E.carotovora subsp. carotovora SCC3193 (PubMed:14742872). Induced by
CC P.syringae pv. tomato DC3000 (PubMed:17965588, PubMed:22325892).
CC Stimulated by ATX1 (PubMed:17965588). Up-regulated by E.amylovora
CC (PubMed:22316300). Accumulates during leaf and flower senescence
CC (PubMed:17310369). Induced expression upon simultaneous feeding by
CC caterpillars (e.g. P.xylostella) and aphids (e.g. B.brassicae) at a low
CC density, but lower levels in plants induced with both caterpillars and
CC a high aphid density (PubMed:25339349). Responsive to rhizobacterium
CC B.cereus AR156 in leaves (PubMed:26433201). Regulated by ATX1 by
CC epigenetic histone H3 methylation (PubMed:18375658).
CC {ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:17310369,
CC ECO:0000269|PubMed:17965588, ECO:0000269|PubMed:18375658,
CC ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892,
CC ECO:0000269|PubMed:23067202, ECO:0000269|PubMed:23603962,
CC ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:25339349,
CC ECO:0000269|PubMed:26433201}.
CC -!- PTM: Phosphorylated and destabilized by ASK7/BIN2.
CC {ECO:0000269|PubMed:28576847}.
CC -!- DISRUPTION PHENOTYPE: Slightly reduced in size (PubMed:17310369). In
CC wrky70-1 mutant, not alteration of responses to both JA and SA
CC (PubMed:18713432). Activation of jasmonic acid (JA)-responsive genes in
CC a COI1-dependent manner (PubMed:14742872). Enhanced disease
CC susceptibility to the necrotrophic bacterial pathogen E.carotovora
CC subsp. carotovora SCC3193. Impaired resistance to the salicylic acid
CC (SA)-monitored fungal pathogen E.cichoracearum. Enhanced JA-induced
CC accumulation of anthocyanins (PubMed:16623907). Compromised basal
CC defense and reduced RPP4-dependent late up-regulation (LURP) of
CC resistance genes (e.g. CML10/CaBP22 and LURP1) upon infection by
CC H.parasitica. Reduced INA- (2,6-dichloroisonicotinic acid, SA analog)
CC mediated resistance toward H.parasitica (PubMed:17313163). Promotion of
CC both developmentally and dark-induced leaf senescence associated with
CC abnormal expression levels of several senescence-associated markers
CC genes (PubMed:17310369, PubMed:22268143). The double mutant wrky54
CC wrky70 exhibits stronger leaf senescence symptoms (PubMed:22268143).
CC Almost no symptoms in response to E.amylovora (PubMed:22316300).
CC Increased susceptibility to P.syringae associated with reduced PR1
CC induction in double mutants wrky46 wrky70 and wrky46 wrky53, and triple
CC mutant wrky46 wrky70 wrky53. In these mutants, higher induction of
CC PDF1.2 upon jasmonic acid (MeJA) treatment (PubMed:22325892). The
CC double mutant wrky54 wrky70 exhibits an enhanced tolerance to osmotic
CC stress associated with improved water retention and enhanced stomatal
CC closure as well as salicylic acid (SA) accumulation, but a reduced
CC induction of osmotic stress-responsive genes and reduced accumulation
CC of the osmoprotectant proline (PubMed:23815736). Unstressed wrky54
CC wrky70 double mutant exhibits increased levels of SA, moderate
CC accumulation of hydrogen peroxide H(2)O(2) and up-regulated expression
CC of both SA and JA/ethylene (ET) responsive defense related genes; thus
CC promoting cell wall fortification and consequently enhancing resistance
CC to necrotrophic pathogens (e.g. P.carotovorum and B.cinerea) associated
CC with reduced cell death, but is not sufficient to trigger
CC hypersensitive reaction (HR)-like cell death and resistance to
CC biotrophic/hemibiotrophic pathogens (e.g. P.syringae), characterized by
CC reduced amount of callose (PubMed:28837631). Reduced rhizobacterium
CC B.cereus AR156-induced systemic resistance (ISR) to P.syringae pv.
CC tomato DC3000 associated with reduced (SA)-mediated signal pathway.
CC Plants lacking both WRKY11 and WRKY70 are totally impaired in B.cereus
CC AR156-mediated ISR (PubMed:26433201). The triple mutant wrky46 wrky54
CC wrky70 has defects in brassinosteroid (BR)-regulated growth and is more
CC tolerant to drought stress (PubMed:28576847).
CC {ECO:0000269|PubMed:14742872, ECO:0000269|PubMed:16623907,
CC ECO:0000269|PubMed:17310369, ECO:0000269|PubMed:17313163,
CC ECO:0000269|PubMed:18713432, ECO:0000269|PubMed:22268143,
CC ECO:0000269|PubMed:22316300, ECO:0000269|PubMed:22325892,
CC ECO:0000269|PubMed:23815736, ECO:0000269|PubMed:26433201,
CC ECO:0000269|PubMed:28576847, ECO:0000269|PubMed:28837631}.
CC -!- SIMILARITY: Belongs to the WRKY group III family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF421157; AAL13046.1; -; mRNA.
DR EMBL; AL163972; CAB88043.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79517.1; -; Genomic_DNA.
DR EMBL; AY039933; AAK64037.1; -; mRNA.
DR EMBL; AY142566; AAN13135.1; -; mRNA.
DR EMBL; AY087389; AAM64939.1; -; mRNA.
DR PIR; T49041; T49041.
DR RefSeq; NP_191199.1; NM_115498.4.
DR AlphaFoldDB; Q9LY00; -.
DR SMR; Q9LY00; -.
DR BioGRID; 10123; 1.
DR IntAct; Q9LY00; 1.
DR STRING; 3702.AT3G56400.1; -.
DR PaxDb; Q9LY00; -.
DR PRIDE; Q9LY00; -.
DR EnsemblPlants; AT3G56400.1; AT3G56400.1; AT3G56400.
DR GeneID; 824807; -.
DR Gramene; AT3G56400.1; AT3G56400.1; AT3G56400.
DR KEGG; ath:AT3G56400; -.
DR Araport; AT3G56400; -.
DR TAIR; locus:2102539; AT3G56400.
DR eggNOG; ENOG502RYCZ; Eukaryota.
DR HOGENOM; CLU_066547_0_0_1; -.
DR InParanoid; Q9LY00; -.
DR OMA; CNTNAET; -.
DR OrthoDB; 891144at2759; -.
DR PhylomeDB; Q9LY00; -.
DR PRO; PR:Q9LY00; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LY00; baseline and differential.
DR Genevisible; Q9LY00; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010120; P:camalexin biosynthetic process; IMP:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR GO; GO:0002229; P:defense response to oomycetes; IMP:UniProtKB.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0080187; P:floral organ senescence; IEP:UniProtKB.
DR GO; GO:0009759; P:indole glucosinolate biosynthetic process; IMP:TAIR.
DR GO; GO:0009682; P:induced systemic resistance; IMP:UniProtKB.
DR GO; GO:0009864; P:induced systemic resistance, jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IMP:UniProtKB.
DR GO; GO:1900056; P:negative regulation of leaf senescence; IGI:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0080151; P:positive regulation of salicylic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:1900457; P:regulation of brassinosteroid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0031347; P:regulation of defense response; IMP:UniProtKB.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0047484; P:regulation of response to osmotic stress; IMP:UniProtKB.
DR GO; GO:2000070; P:regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0090333; P:regulation of stomatal closure; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0009625; P:response to insect; IEP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IEP:UniProtKB.
DR GO; GO:0000302; P:response to reactive oxygen species; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IMP:TAIR.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31282; PTHR31282; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; DNA-binding; Ethylene signaling pathway;
KW Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..294
FT /note="Probable WRKY transcription factor 70"
FT /id="PRO_0000133711"
FT DNA_BIND 114..182
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 72..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 201..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 90..97
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 201..227
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 210
FT /note="S -> T (in Ref. 5; AAM64939)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="E -> EE (in Ref. 5; AAM64939)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 294 AA; 32936 MW; C6663D5CD2C8EB43 CRC64;
MDTNKAKKLK VMNQLVEGHD LTTQLQQLLS QPGSGLEDLV AKILVCFNNT ISVLDTFEPI
SSSSSLAAVE GSQNASCDND GKFEDSGDSR KRLGPVKGKR GCYKRKKRSE TCTIESTILE
DAFSWRKYGQ KEILNAKFPR SYFRCTHKYT QGCKATKQVQ KVELEPKMFS ITYIGNHTCN
TNAETPKSKT CDHHDEIFMD SEDHKSPSLS TSMKEEDNPH RHHGSSTEND LSLVWPEMVF
EEDYHHQASY VNGKTSTSID VLGSQDLMVF GGGGDFEFSE NEHFSIFSSC SNLS
