WRK71_ORYSI
ID WRK71_ORYSI Reviewed; 348 AA.
AC Q6IEL0;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=WRKY transcription factor WRKY71 {ECO:0000303|PubMed:15047897};
DE Short=OsWRKY71 {ECO:0000303|PubMed:15047897};
GN Name=WRKY71 {ECO:0000303|PubMed:15047897};
GN ORFNames=OsI_06106 {ECO:0000312|EMBL:EAY84736.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, AND REGULATION BY GIBBERELLIC ACID.
RX PubMed=15047897; DOI=10.1104/pp.103.034967;
RA Zhang Z.-L., Xie Z., Zou X., Casaretto J., Ho T.-H.D., Shen Q.J.;
RT "A rice WRKY gene encodes a transcriptional repressor of the gibberellin
RT signaling pathway in aleurone cells.";
RL Plant Physiol. 134:1500-1513(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY DEFENSE SIGNALING
RP MOLECULES, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Guang-Lu-Ai No.4; TISSUE=Leaf;
RX PubMed=16919842; DOI=10.1016/j.jplph.2006.07.006;
RA Liu X., Bai X., Wang X., Chu C.;
RT "OsWRKY71, a rice transcription factor, is involved in rice defense
RT response.";
RL J. Plant Physiol. 164:969-979(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [5]
RP INDUCTION BY ABA.
RC STRAIN=cv. IR29, and cv. Pokkali;
RX PubMed=25110688; DOI=10.1155/2014/706890;
RA Basu S., Roychoudhury A.;
RT "Expression profiling of abiotic stress-inducible genes in response to
RT multiple stresses in rice (Oryza sativa L.) varieties with contrasting
RT level of stress tolerance.";
RL Biomed. Res. Int. 2014:706890-706890(2014).
CC -!- FUNCTION: Transcription repressor. Interacts specifically with the W
CC box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive
CC cis-acting element. Represses specifically gibberellic acid (GA)-
CC induced promoters in aleurone cells, probably by interfering with GAM1
CC (PubMed:15047897). Regulates, probably indirectly, the activation of
CC defense-related genes such as GF14E during defense response (By
CC similarity). Modulates plant innate immunity against X.oryzae pv.
CC oryzae (Xoo) (By similarity). Confers resistance to the virulent
CC bacterial pathogen X.oryzae pv. oryzae (Xoo) 13751, probably via the
CC regulation of NPR1 and PR1b defense signaling pathways
CC (PubMed:16919842). {ECO:0000250|UniProtKB:Q6QHD1,
CC ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16919842}.
CC -!- SUBUNIT: Interacts with WRKY51; this interaction promotes W box binding
CC of the complex WRKY51/WRKY71 in a zinc ion-dependent manner.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:15047897}. Note=Localized in nuclei of aleurone
CC cells. {ECO:0000269|PubMed:15047897}.
CC -!- TISSUE SPECIFICITY: Highly expressed in aleurone cells
CC (PubMed:15047897). In seeds, predominantly present in the plumule,
CC radicle and scutellum of the embryo. Expressed in roots, stems, young
CC leaves and spikelets (PubMed:16919842). {ECO:0000269|PubMed:15047897,
CC ECO:0000269|PubMed:16919842}.
CC -!- INDUCTION: Induced by biotic elicitors (e.g. fungal chitin
CC oligosaccharide and fungal cerebroside elicitors) and pathogen
CC infection (e.g. the compatible pathogenic fungus M.grisea race 007,
CC M.grisea crabgrass BR29). Accumulates in response to M.oryzae (By
CC similarity). Triggered by defense signaling molecules, such as
CC salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-
CC carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae)
CC (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein
CC level) (PubMed:15047897). Induced by abscisic acid (ABA) in aleurone
CC cells, roots and leaves (PubMed:25110688). Accumulates in response to
CC uniconazole, a GA biosynthesis inhibitor. Triggered strongly by cold in
CC leaves, stems and developing spikes, but moderately by drought and salt
CC stresses (By similarity). {ECO:0000250|UniProtKB:Q6QHD1,
CC ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16919842,
CC ECO:0000269|PubMed:25110688}.
CC -!- DOMAIN: The WRKY domain (213-266) is required to bind DNA.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- DOMAIN: The C-terminal region (267-348) is required for the repressing
CC activity on gibberellic acid (GA)-induced promoters.
CC {ECO:0000250|UniProtKB:Q6QHD1}.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BK005074; DAA05136.1; -; Genomic_DNA.
DR EMBL; AY676927; AAT84158.1; -; mRNA.
DR EMBL; CM000127; EAY84736.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6IEL0; -.
DR SMR; Q6IEL0; -.
DR STRING; 39946.Q6IEL0; -.
DR EnsemblPlants; BGIOSGA007670-TA; BGIOSGA007670-PA; BGIOSGA007670.
DR Gramene; BGIOSGA007670-TA; BGIOSGA007670-PA; BGIOSGA007670.
DR HOGENOM; CLU_047067_0_0_1; -.
DR OMA; DPWISTQ; -.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IDA:Gramene.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:Gramene.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IMP:Gramene.
DR GO; GO:0050832; P:defense response to fungus; IEA:EnsemblPlants.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009685; P:gibberellin metabolic process; IEP:Gramene.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR GO; GO:0031347; P:regulation of defense response; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:Gramene.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0010200; P:response to chitin; ISS:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEA:EnsemblPlants.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IDA:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IEA:EnsemblPlants.
DR GO; GO:0002238; P:response to molecule of fungal origin; IEA:EnsemblPlants.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IEA:EnsemblPlants.
DR GO; GO:0009414; P:response to water deprivation; IEA:EnsemblPlants.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Gibberellin signaling pathway; Nucleus;
KW Plant defense; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..348
FT /note="WRKY transcription factor WRKY71"
FT /id="PRO_0000436959"
FT DNA_BIND 187..253
FT /note="WRKY"
FT /evidence="ECO:0000250|UniProtKB:Q6QHD1,
FT ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 91..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..348
FT /note="Transcription repression of gibberellic acid (GA)-
FT induced promoters"
FT /evidence="ECO:0000250|UniProtKB:Q6QHD1"
FT COILED 50..84
FT /evidence="ECO:0000255"
FT MOTIF 116..122
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 91..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..139
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 348 AA; 37258 MW; BA7BC1EDF6C1E28C CRC64;
MDPWISTQPS LSLDLRVGLP ATAAVAMVKP KVLVEEDFFH QQPLKKDPEV AALEAELKRM
GAENRQLSEM LAAVAAKYEA LQSQFSDMVT ASANNGGGGG NNQSSTSEGG SVSPSRKRKS
ESLDDSPPPP PPPHPHAAPH HMHVMPGAAA AGYADQTECT SGEPCKRIRE ECKPKISKLY
VHADPSDLSL VVKDGYQWRK YGQKVTKDNP CPRAYFRCSF APACPVKKKV QRSAEDNTIL
VATYEGEHNH GQPPPPLQSA AQNSDGSGKS AGKPPHAPAA APPAPVVPHR QHEPVVVNGE
QQAAAASEMI RRNLAEQMAM TLTRDPSFKA ALVTALSGRI LELSPTKD
