WRK71_ORYSJ
ID WRK71_ORYSJ Reviewed; 348 AA.
AC Q6QHD1; A0A0P0VFU9;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=WRKY transcription factor WRKY71 {ECO:0000303|PubMed:18175928};
DE Short=OsWRKY71 {ECO:0000303|PubMed:18175928};
GN Name=WRKY71 {ECO:0000303|PubMed:18175928};
GN Synonyms=wrky38 {ECO:0000303|Ref.2};
GN OrderedLocusNames=Os02g0181300 {ECO:0000312|EMBL:BAF08008.1};
GN ORFNames=OJ1297_C09.15 {ECO:0000312|EMBL:BAD25136.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION BY BIOTIC ELICITORS AND
RP PATHOGEN INFECTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18175928; DOI=10.1271/bbb.70553;
RA Chujo T., Kato T., Yamada K., Takai R., Akimoto-Tomiyama C., Minami E.,
RA Nagamura Y., Shibuya N., Yasuda M., Nakashita H., Umemura K., Okada A.,
RA Okada K., Nojiri H., Yamane H.;
RT "Characterization of an elicitor-induced rice WRKY gene, OsWRKY71.";
RL Biosci. Biotechnol. Biochem. 72:240-245(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Mazzucotelli E., Mare C., Cattivelli L.;
RT "Hv-Wrky38 transcription factor involved in cold and drought response in
RT barley.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP INDUCTION BY COLD; DROUGHT AND SALT STRESSES.
RX PubMed=14645724; DOI=10.1104/pp.103.025742;
RA Rabbani M.A., Maruyama K., Abe H., Khan M.A., Katsura K., Ito Y.,
RA Yoshiwara K., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Monitoring expression profiles of rice genes under cold, drought, and
RT high-salinity stresses and abscisic acid application using cDNA microarray
RT and RNA gel-blot analyses.";
RL Plant Physiol. 133:1755-1767(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND REGULATION BY
RP GIBBERELLIC ACID.
RX PubMed=15047897; DOI=10.1104/pp.103.034967;
RA Zhang Z.-L., Xie Z., Zou X., Casaretto J., Ho T.-H.D., Shen Q.J.;
RT "A rice WRKY gene encodes a transcriptional repressor of the gibberellin
RT signaling pathway in aleurone cells.";
RL Plant Physiol. 134:1500-1513(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=15629062; DOI=10.1186/1471-2148-5-1;
RA Zhang Y., Wang L.;
RT "The WRKY transcription factor superfamily: its origin in eukaryotes and
RT expansion in plants.";
RL BMC Evol. Biol. 5:1-1(2005).
RN [9]
RP GENE FAMILY.
RX PubMed=16106749; DOI=10.1093/dnares/12.1.9;
RA Wu K.-L., Guo Z.-J., Wang H.-H., Li J.;
RT "The WRKY family of transcription factors in rice and Arabidopsis and their
RT origins.";
RL DNA Res. 12:9-26(2005).
RN [10]
RP INDUCTION BY ABSCISIC ACID, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15618416; DOI=10.1104/pp.104.054312;
RA Xie Z., Zhang Z.-L., Zou X., Huang J., Ruas P., Thompson D., Shen Q.J.;
RT "Annotations and functional analyses of the rice WRKY gene superfamily
RT reveal positive and negative regulators of abscisic acid signaling in
RT aleurone cells.";
RL Plant Physiol. 137:176-189(2005).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 198-TRP--TYR-201 AND
RP 248-HIS--HIS-250, INTERACTION WITH WRKY51, TISSUE SPECIFICITY, INDUCTION BY
RP ABSCISIC ACID AND UNICONAZOLE, AND DOMAIN.
RC STRAIN=cv. Nipponbare;
RX PubMed=16623886; DOI=10.1111/j.1365-313x.2006.02694.x;
RA Xie Z., Zhang Z.L., Zou X., Yang G., Komatsu S., Shen Q.J.;
RT "Interactions of two abscisic-acid induced WRKY genes in repressing
RT gibberellin signaling in aleurone cells.";
RL Plant J. 46:231-242(2006).
RN [12]
RP FUNCTION, INDUCTION BY DEFENSE SIGNALING MOLECULES, SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16919842; DOI=10.1016/j.jplph.2006.07.006;
RA Liu X., Bai X., Wang X., Chu C.;
RT "OsWRKY71, a rice transcription factor, is involved in rice defense
RT response.";
RL J. Plant Physiol. 164:969-979(2007).
RN [13]
RP INDUCTION BY PATHOGENS, AND GENE FAMILY.
RX PubMed=19772648; DOI=10.1186/1471-2229-9-120;
RA Berri S., Abbruscato P., Faivre-Rampant O., Brasileiro A.C., Fumasoni I.,
RA Satoh K., Kikuchi S., Mizzi L., Morandini P., Pe M.E., Piffanelli P.;
RT "Characterization of WRKY co-regulatory networks in rice and Arabidopsis.";
RL BMC Plant Biol. 9:120-120(2009).
RN [14]
RP FUNCTION, AND REPRESSION BY GIBBERELLIC ACID.
RC STRAIN=cv. M-104, and cv. Tainung 67;
RX PubMed=19199048; DOI=10.1007/s11103-009-9463-4;
RA Zhang Z.L., Shin M., Zou X., Huang J., Ho T.H., Shen Q.J.;
RT "A negative regulator encoded by a rice WRKY gene represses both abscisic
RT acid and gibberellins signaling in aleurone cells.";
RL Plant Mol. Biol. 70:139-151(2009).
RN [15]
RP FUNCTION.
RX PubMed=21961049; DOI=10.1007/s12284-010-9039-6;
RA Peng Y., Bartley L.E., Canlas P., Ronald P.C.;
RT "OsWRKY IIa transcription factors modulate rice innate immunity.";
RL Rice 3:36-42(2010).
RN [16]
RP INDUCTION BY MAGNAPORTHE ORYZAE.
RC STRAIN=cv. Nipponbare;
RX PubMed=21726398; DOI=10.1111/j.1364-3703.2011.00731.x;
RA Delteil A., Blein M., Faivre-Rampant O., Guellim A., Estevan J., Hirsch J.,
RA Bevitori R., Michel C., Morel J.B.;
RT "Building a mutant resource for the study of disease resistance in rice
RT reveals the pivotal role of several genes involved in defence.";
RL Mol. Plant Pathol. 13:72-82(2012).
RN [17]
RP INDUCTION BY COLD.
RX PubMed=23495849; DOI=10.1111/pbi.12056;
RA Kovalchuk N., Jia W., Eini O., Morran S., Pyvovarenko T., Fletcher S.,
RA Bazanova N., Harris J., Beck-Oldach K., Shavrukov Y., Langridge P.,
RA Lopato S.;
RT "Optimization of TaDREB3 gene expression in transgenic barley using cold-
RT inducible promoters.";
RL Plant Biotechnol. J. 11:659-670(2013).
RN [18]
RP INDUCTION BY BIOTIC ELICITORS.
RC STRAIN=cv. Nipponbare;
RX PubMed=23462973; DOI=10.1007/s11103-013-0032-5;
RA Chujo T., Miyamoto K., Shimogawa T., Shimizu T., Otake Y., Yokotani N.,
RA Nishizawa Y., Shibuya N., Nojiri H., Yamane H., Minami E., Okada K.;
RT "OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate
RT immune responses in rice against rice blast fungus.";
RL Plant Mol. Biol. 82:23-37(2013).
RN [19]
RP FUNCTION.
RX PubMed=26851365; DOI=10.1016/j.bbrc.2016.02.005;
RA Liu Q., Yang J., Zhang S., Zhao J., Feng A., Yang T., Wang X., Mao X.,
RA Dong J., Zhu X., Leung H., Leach J.E., Liu B.;
RT "OsGF14e positively regulates panicle blast resistance in rice.";
RL Biochem. Biophys. Res. Commun. 471:247-252(2016).
RN [20]
RP FUNCTION.
RX PubMed=26467468; DOI=10.1094/mpmi-03-15-0047-r;
RA Liu Q., Yang J., Zhang S., Zhao J., Feng A., Yang T., Wang X., Mao X.,
RA Dong J., Zhu X., Leung H., Leach J.E., Liu B.;
RT "OsGF14b positively regulates panicle blast resistance but negatively
RT regulates leaf blast resistance in rice.";
RL Mol. Plant Microbe Interact. 29:46-56(2016).
CC -!- FUNCTION: Transcription repressor. Interacts specifically with the W
CC box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive
CC cis-acting element (PubMed:18175928, PubMed:15047897, PubMed:16623886).
CC Represses specifically gibberellic acid (GA)-induced promoters in
CC aleurone cells, probably by interfering with GAM1 (PubMed:15047897,
CC PubMed:16623886, PubMed:19199048). Regulates, probably indirectly, the
CC activation of defense-related genes such as GF14E during defense
CC response (PubMed:18175928, PubMed:26851365, PubMed:26467468). Modulates
CC plant innate immunity against X.oryzae pv. oryzae (Xoo)
CC (PubMed:21961049). Confers resistance to the virulent bacterial
CC pathogen Xoo 13751, probably via the regulation of NPR1 and PR1b
CC defense signaling pathways (PubMed:16919842).
CC {ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16623886,
CC ECO:0000269|PubMed:16919842, ECO:0000269|PubMed:18175928,
CC ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:21961049,
CC ECO:0000269|PubMed:26467468, ECO:0000269|PubMed:26851365}.
CC -!- SUBUNIT: Interacts with WRKY51; this interaction promotes W box binding
CC of the complex WRKY51/WRKY71 in a zinc ion-dependent manner.
CC {ECO:0000269|PubMed:16623886}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:15047897, ECO:0000269|PubMed:16919842,
CC ECO:0000269|PubMed:18175928}. Note=Localized in nuclei of aleurone
CC cells. {ECO:0000269|PubMed:15047897}.
CC -!- TISSUE SPECIFICITY: Highly expressed in aleurone cells
CC (PubMed:15047897, PubMed:16623886). In seeds, predominantly present in
CC the plumule, radicle and scutellum of the embryo (PubMed:16623886).
CC Expressed in roots, stems, young leaves and spikelets
CC (PubMed:16919842). {ECO:0000269|PubMed:15047897,
CC ECO:0000269|PubMed:16623886, ECO:0000269|PubMed:16919842}.
CC -!- INDUCTION: Induced by biotic elicitors (e.g. fungal chitin
CC oligosaccharide and fungal cerebroside elicitors) and pathogen
CC infection (e.g. the compatible pathogenic fungus M.grisea race 007, M.
CC grisea crabgrass BR29) (PubMed:18175928, PubMed:19772648,
CC PubMed:23462973). Accumulates in response to M.oryzae
CC (PubMed:21726398). Triggered by defense signaling molecules, such as
CC salicylic acid (SA), methyl jasmonate (MeJA), 1-aminocyclo-propane-1-
CC carboxylic acid (ACC), wounding and pathogen infection (e.g. X.oryzae)
CC (PubMed:16919842). Repressed by gibberellic acid (GA) (at protein
CC level) (PubMed:15047897, PubMed:19199048). Induced by abscisic acid
CC (ABA) in aleurone cells, roots and leaves (PubMed:15618416,
CC PubMed:16623886). Accumulates in response to uniconazole, a GA
CC biosynthesis inhibitor (PubMed:16623886). Triggered strongly by cold in
CC leaves, stems and developing spikes, but moderately by drought and salt
CC stresses (PubMed:14645724, PubMed:23495849).
CC {ECO:0000269|PubMed:14645724, ECO:0000269|PubMed:15047897,
CC ECO:0000269|PubMed:15618416, ECO:0000269|PubMed:16623886,
CC ECO:0000269|PubMed:16919842, ECO:0000269|PubMed:18175928,
CC ECO:0000269|PubMed:19199048, ECO:0000269|PubMed:19772648,
CC ECO:0000269|PubMed:21726398, ECO:0000269|PubMed:23462973,
CC ECO:0000269|PubMed:23495849}.
CC -!- DOMAIN: The WRKY domain (213-266) is required to bind DNA.
CC {ECO:0000269|PubMed:16623886}.
CC -!- DOMAIN: The C-terminal region (267-348) is required for the repressing
CC activity on gibberellic acid (GA)-induced promoters.
CC {ECO:0000269|PubMed:16623886}.
CC -!- DISRUPTION PHENOTYPE: Impaired repression activity on gibberellic acid
CC (GA)-induced promoters. {ECO:0000269|PubMed:16623886}.
CC -!- SIMILARITY: Belongs to the WRKY group II-a family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAS77308.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB190817; BAF80893.1; -; mRNA.
DR EMBL; AY541677; AAS48546.1; -; Genomic_DNA.
DR EMBL; AP004087; BAD25136.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08008.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS77308.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_015627417.1; XM_015771931.1.
DR AlphaFoldDB; Q6QHD1; -.
DR SMR; Q6QHD1; -.
DR STRING; 4530.OS02T0181300-01; -.
DR PRIDE; Q6QHD1; -.
DR EnsemblPlants; Os02t0181300-01; Os02t0181300-01; Os02g0181300.
DR GeneID; 4328512; -.
DR Gramene; Os02t0181300-01; Os02t0181300-01; Os02g0181300.
DR KEGG; osa:4328512; -.
DR InParanoid; Q6QHD1; -.
DR OrthoDB; 1185192at2759; -.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009938; P:negative regulation of gibberellic acid mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0031347; P:regulation of defense response; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IDA:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0010200; P:response to chitin; IEP:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR GO; GO:0009620; P:response to fungus; IEP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IDA:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IEP:UniProtKB.
DR GO; GO:0002238; P:response to molecule of fungal origin; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IEP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IEP:UniProtKB.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Gibberellin signaling pathway; Nucleus;
KW Plant defense; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..348
FT /note="WRKY transcription factor WRKY71"
FT /id="PRO_0000436958"
FT DNA_BIND 187..253
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223,
FT ECO:0000269|PubMed:16623886"
FT REGION 91..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..348
FT /note="Transcription repression of gibberellic acid (GA)-
FT induced promoters"
FT /evidence="ECO:0000269|PubMed:16623886"
FT COILED 50..84
FT /evidence="ECO:0000255"
FT MOTIF 116..122
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255, ECO:0000303|PubMed:15618416"
FT COMPBIAS 91..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..139
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 198..201
FT /note="WRKY->RRAD: Slightly reduced repression activity on
FT gibberellic acid (GA)-induced promoters, but impaired
FT binding to gibberellic acid (GA)-induced promoters."
FT /evidence="ECO:0000269|PubMed:16623886"
FT MUTAGEN 248..250
FT /note="HNH->QNS: Slightly reduced repression activity on
FT gibberellic acid (GA)-induced promoters, but impaired
FT binding to gibberellic acid (GA)-induced promoters."
FT /evidence="ECO:0000269|PubMed:16623886"
SQ SEQUENCE 348 AA; 37227 MW; FA7B71EDF6C19590 CRC64;
MDPWISTQPS LSLDLRVGLP ATAAVAMVKP KVLVEEDFFH QQPLKKDPEV AALEAELKRM
GAENRQLSEM LAAVAAKYEA LQSQFSDMVT ASANNGGGGG NNPSSTSEGG SVSPSRKRKS
ESLDDSPPPP PPPHPHAAPH HMHVMPGAAA AGYADQTECT SGEPCKRIRE ECKPKISKLY
VHADPSDLSL VVKDGYQWRK YGQKVTKDNP CPRAYFRCSF APACPVKKKV QRSAEDNTIL
VATYEGEHNH GQPPPPLQSA AQNSDGSGKS AGKPPHAPAA APPAPVVPHR QHEPVVVNGE
QQAAAASEMI RRNLAEQMAM TLTRDPSFKA ALVTALSGRI LELSPTKD
