WRKY5_ORYSJ
ID WRKY5_ORYSJ Reviewed; 502 AA.
AC Q75L34; A0A0P0WHU6; Q6IES6;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-AUG-2022, sequence version 2.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Transcription factor WRKY5 {ECO:0000305};
DE Short=OsWRKY5 {ECO:0000303|PubMed:15047897};
DE AltName: Full=WRKY transcription factor 5 {ECO:0000305};
GN Name=WRKY5 {ECO:0000303|PubMed:15047897};
GN OrderedLocusNames=Os05g0137500 {ECO:0000312|EMBL:BAS92167.1},
GN LOC_Os05g04640 {ECO:0000305};
GN ORFNames=OJ1127_B08.14 {ECO:0000312|EMBL:AAS98424.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15047897; DOI=10.1104/pp.103.034967;
RA Zhang Z.-L., Xie Z., Zou X., Casaretto J., Ho T.-H.D., Shen Q.J.;
RT "A rice WRKY gene encodes a transcriptional repressor of the gibberellin
RT signaling pathway in aleurone cells.";
RL Plant Physiol. 134:1500-1513(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION, HOMODIMERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=31505875; DOI=10.3390/ijms20184437;
RA Kim T., Kang K., Kim S.H., An G., Paek N.C.;
RT "OsWRKY5 promotes rice leaf senescence via senescence-associated NAC and
RT abscisic acid biosynthesis pathway.";
RL Int. J. Mol. Sci. 20:0-0(2019).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=34718775; DOI=10.1093/plphys/kiab492;
RA Lim C., Kang K., Shim Y., Yoo S.C., Paek N.C.;
RT "Inactivating transcription factor OsWRKY5 enhances drought tolerance
RT through abscisic acid signaling pathways.";
RL Plant Physiol. 188:1900-1916(2022).
CC -!- FUNCTION: Transcription factor that acts as positive regulator of leaf
CC senescence (PubMed:31505875). Is involved in both the onset and
CC progression of leaf senescence (PubMed:31505875). Upregulates the
CC expression of genes controlling chlorophyll degradation and leaf
CC senescence (PubMed:31505875). Acts as positive regulator of the
CC senescence-associated NAC genes NAC4 and NAC58 (PubMed:31505875). Acts
CC as positive regulator of abscisic acid (ABA) biosynthesis during leaf
CC senescence (PubMed:31505875). Acts as negative regulator of drought
CC tolerance by inhibiting ABA-induced stomatal closure during drought
CC stress (PubMed:34718775). Functions as a direct negative regulator of
CC MYB2 transcription through binding to the repeated W-boxes of the MYB2
CC promoter region (PubMed:34718775). {ECO:0000269|PubMed:31505875,
CC ECO:0000269|PubMed:34718775}.
CC -!- SUBUNIT: Forms homodimers. {ECO:0000269|PubMed:31505875}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00223,
CC ECO:0000269|PubMed:31505875}.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaf blades and leaf sheaths
CC (PubMed:31505875, PubMed:34718775). Expressed in roots, culms and
CC panicles (PubMed:31505875). {ECO:0000269|PubMed:31505875,
CC ECO:0000269|PubMed:34718775}.
CC -!- INDUCTION: Induced in leaves during natural senescence and dark-induced
CC senescence (PubMed:31505875). Down-regulated by abscisic acid (ABA) and
CC jasmonate (MeJA) (PubMed:31505875). Down-regulated by salt, mannitol-
CC induced osmotic stress and drought stress (PubMed:34718775).
CC {ECO:0000269|PubMed:31505875, ECO:0000269|PubMed:34718775}.
CC -!- DISRUPTION PHENOTYPE: The leaves of the oswrky5 mutant plants retain
CC leaf greenness during dark-induced senescence (PubMed:31505875). The
CC mutant plants exhibit tolerance to mannitol-induced osmotic stress
CC (PubMed:31505875). Enhanced tolerance to drought stress
CC (PubMed:34718775). {ECO:0000269|PubMed:31505875,
CC ECO:0000269|PubMed:34718775}.
CC -!- MISCELLANEOUS: The gain-of-function mutant plants oswrky5-D (T-DNA
CC activation tagging) promote leaf senescence under natural and dark-
CC induced senescence conditions (PubMed:31505875). Overexpression of
CC WRKY5 reduces grain yield under normal and drought stress conditions
CC (PubMed:31505875). {ECO:0000269|PubMed:31505875}.
CC -!- SIMILARITY: Belongs to the WRKY group II family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAS92167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BK005008; DAA05070.1; -; Genomic_DNA.
DR EMBL; AC093490; AAS98424.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92167.1; ALT_SEQ; Genomic_DNA.
DR SMR; Q75L34; -.
DR EnsemblPlants; Os05t0137500-00; Os05t0137500-00; Os05g0137500.
DR Gramene; Os05t0137500-00; Os05t0137500-00; Os05g0137500.
DR InParanoid; Q75L34; -.
DR OMA; GENADNR; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProt.
DR Gene3D; 2.20.25.80; -; 1.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR InterPro; IPR044810; WRKY_plant.
DR PANTHER; PTHR31429; PTHR31429; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid signaling pathway; Coiled coil; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..502
FT /note="Transcription factor WRKY5"
FT /id="PRO_0000456286"
FT DNA_BIND 243..309
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT COILED 96..123
FT /evidence="ECO:0000255"
FT CONFLICT 291
FT /note="E -> K (in Ref. 1; DAA05070)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="S -> N (in Ref. 2; AAS98424)"
SQ SEQUENCE 502 AA; 52274 MW; EBFE4BA4F85D1AFC CRC64;
MEMMVQKQRH EEGEEERGGL CAREIKELDF FSAAGAGAGR RDDDDVLRAD GISSSHAGFM
VSTALDLLTA VNDGDHHEEK KGQSNIHQSK QMDAAATTVE GELRQAGEEN RRLRRRLEEL
TSSYGALYHQ LVQAQQLHTK HQQQAPIAGV QLLDALAAAS PASHRRRAAA AVDGDRTADS
DGGEGDENVS PSLGSKRPAA AATLTRLTPE SGSGGENNGG GEQAPAAEMA PCRKARVSVR
ARSEAPMISD GCQWRKYGQK MAKGNPCPRA YYRCTMASQC PVRKQVQRCA EDKSILITTY
EGTHSHPLPP AAAAMAKTTS AAAAMLLSGP AVSRDALFAA HHHVVAPPPF FHHPYAGSTM
ATLSASAPFP TITLDLTQPP PTTTTTAAAA MLQLHRPYAF SSLPFSMYGA GGGSHRPPVV
LPPPSSVVET MTAAITRDPN FTTAVAAALS SIMAGGGAQA RTPPRGGSDA AGDINGGGGA
DHATAGARAA AAATQPCGTS PT
