WRP73_HUMAN
ID WRP73_HUMAN Reviewed; 460 AA.
AC Q9P2S5; Q5T0D6; Q9BUH7; Q9NTK7; Q9NX56;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=WD repeat-containing protein WRAP73;
DE AltName: Full=WD repeat-containing protein 8;
DE AltName: Full=WD repeat-containing protein antisense to TP73 gene;
GN Name=WRAP73; Synonyms=WDR8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11401440; DOI=10.1006/geno.2000.6475;
RA Koshizuka Y., Ikegawa S., Sano M., Nakamura K., Nakamura Y.;
RT "Isolation, characterization, and mapping of the mouse and human WDR8
RT genes, members of a novel WD-repeat gene family.";
RL Genomics 72:252-259(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Signet-ring cell carcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-281, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH SSX2IP.
RX PubMed=26545777; DOI=10.1016/j.bbrc.2015.10.169;
RA Hori A., Morand A., Ikebe C., Frith D., Snijders A.P., Toda T.;
RT "The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and
RT ensures proper spindle length and orientation.";
RL Biochem. Biophys. Res. Commun. 468:39-45(2015).
RN [8]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=26675238; DOI=10.1242/jcs.179713;
RA Kurtulmus B., Wang W., Ruppert T., Neuner A., Cerikan B., Viol L.,
RA Duenas-Sanchez R., Gruss O.J., Pereira G.;
RT "WDR8 is a centriolar satellite and centriole-associated protein that
RT promotes ciliary vesicle docking during ciliogenesis.";
RL J. Cell Sci. 129:621-636(2016).
CC -!- FUNCTION: The SSX2IP:WRAP73 complex is proposed to act as regulator of
CC spindle anchoring at the mitotic centrosome. Required for the
CC centrosomal localization of SSX2IP and normal mitotic bipolar spindle
CC morphology (PubMed:26545777). Required for the targeting of centriole
CC satellite proteins to centrosomes such as of PCM1, SSX2IP, CEP290 and
CC PIBF1/CEP90. Required for ciliogenesis and involved in the removal of
CC the CEP97:CCP110 complex from the mother centriole. Involved in ciliary
CC vesicle formation at the mother centriole and required for the docking
CC of vesicles to the basal body during ciliogenesis; may promote docking
CC of RAB8A- and ARL13B-containing vesicles (PubMed:26675238).
CC {ECO:0000269|PubMed:26545777, ECO:0000269|PubMed:26675238}.
CC -!- SUBUNIT: Interacts with SSX2IP. {ECO:0000269|PubMed:26545777}.
CC -!- INTERACTION:
CC Q9P2S5; Q9Y2D8: SSX2IP; NbExp=9; IntAct=EBI-1054904, EBI-2212028;
CC Q9P2S5; Q8VC66: Ssx2ip; Xeno; NbExp=4; IntAct=EBI-1054904, EBI-6654049;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome {ECO:0000269|PubMed:14654843,
CC ECO:0000269|PubMed:26545777, ECO:0000269|PubMed:26675238}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome, centriole
CC {ECO:0000269|PubMed:26675238}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriolar satellite
CC {ECO:0000269|PubMed:26675238}. Note=Enriched in the proximal end of the
CC mother centriole (PubMed:26545777). During ciliogenesis also associated
CC with the basal body of the adjacent centriole (PubMed:26675238).
CC {ECO:0000269|PubMed:26545777, ECO:0000269|PubMed:26675238}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Predominant expression in heart, brain,
CC liver, thymus, prostate, and testis, and barely detectable expression
CC in lung.
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DR EMBL; AB034912; BAA92312.1; -; mRNA.
DR EMBL; AK000437; BAA91164.1; -; mRNA.
DR EMBL; AL136528; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL513320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC086311; AAH86311.1; -; mRNA.
DR CCDS; CCDS48.1; -.
DR RefSeq; NP_060288.3; NM_017818.3.
DR AlphaFoldDB; Q9P2S5; -.
DR SMR; Q9P2S5; -.
DR BioGRID; 119068; 132.
DR IntAct; Q9P2S5; 174.
DR MINT; Q9P2S5; -.
DR STRING; 9606.ENSP00000270708; -.
DR iPTMnet; Q9P2S5; -.
DR PhosphoSitePlus; Q9P2S5; -.
DR BioMuta; WRAP73; -.
DR DMDM; 17380347; -.
DR EPD; Q9P2S5; -.
DR jPOST; Q9P2S5; -.
DR MassIVE; Q9P2S5; -.
DR MaxQB; Q9P2S5; -.
DR PaxDb; Q9P2S5; -.
DR PeptideAtlas; Q9P2S5; -.
DR PRIDE; Q9P2S5; -.
DR ProteomicsDB; 83889; -.
DR Antibodypedia; 26895; 49 antibodies from 16 providers.
DR DNASU; 49856; -.
DR Ensembl; ENST00000270708.12; ENSP00000270708.7; ENSG00000116213.16.
DR GeneID; 49856; -.
DR KEGG; hsa:49856; -.
DR MANE-Select; ENST00000270708.12; ENSP00000270708.7; NM_017818.4; NP_060288.3.
DR UCSC; uc001ako.4; human.
DR CTD; 49856; -.
DR GeneCards; WRAP73; -.
DR HGNC; HGNC:12759; WRAP73.
DR HPA; ENSG00000116213; Low tissue specificity.
DR MIM; 606040; gene.
DR neXtProt; NX_Q9P2S5; -.
DR OpenTargets; ENSG00000116213; -.
DR PharmGKB; PA37363; -.
DR VEuPathDB; HostDB:ENSG00000116213; -.
DR eggNOG; KOG4497; Eukaryota.
DR GeneTree; ENSGT00390000003623; -.
DR InParanoid; Q9P2S5; -.
DR OMA; LAICTGN; -.
DR OrthoDB; 764000at2759; -.
DR PhylomeDB; Q9P2S5; -.
DR TreeFam; TF329103; -.
DR PathwayCommons; Q9P2S5; -.
DR SignaLink; Q9P2S5; -.
DR BioGRID-ORCS; 49856; 39 hits in 1072 CRISPR screens.
DR ChiTaRS; WRAP73; human.
DR GeneWiki; WDR8; -.
DR GenomeRNAi; 49856; -.
DR Pharos; Q9P2S5; Tbio.
DR PRO; PR:Q9P2S5; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9P2S5; protein.
DR Bgee; ENSG00000116213; Expressed in granulocyte and 181 other tissues.
DR ExpressionAtlas; Q9P2S5; baseline and differential.
DR Genevisible; Q9P2S5; HS.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005815; C:microtubule organizing center; IBA:GO_Central.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:1990811; C:MWP complex; IBA:GO_Central.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0090307; P:mitotic spindle assembly; IMP:UniProtKB.
DR GO; GO:1902857; P:positive regulation of non-motile cilium assembly; IMP:UniProtKB.
DR GO; GO:1902440; P:protein localization to mitotic spindle pole body; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
PE 1: Evidence at protein level;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..460
FT /note="WD repeat-containing protein WRAP73"
FT /id="PRO_0000051356"
FT REPEAT 46..86
FT /note="WD 1"
FT REPEAT 89..129
FT /note="WD 2"
FT REPEAT 176..210
FT /note="WD 3"
FT REPEAT 221..260
FT /note="WD 4"
FT REPEAT 328..369
FT /note="WD 5"
FT REPEAT 371..410
FT /note="WD 6"
FT MOD_RES 281
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 331
FT /note="I -> M (in dbSNP:rs2760320)"
FT /id="VAR_057624"
FT VARIANT 358
FT /note="V -> I (in dbSNP:rs16823940)"
FT /id="VAR_057625"
FT CONFLICT 381
FT /note="W -> R (in Ref. 2; BAA91164)"
FT /evidence="ECO:0000305"
FT CONFLICT 405
FT /note="C -> R (in Ref. 2; BAA91164)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 460 AA; 51588 MW; C2E318B1F06F79BF CRC64;
MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS
LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS
LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD
LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV
GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP
LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD
IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGDFAVLS
LCWHLSGDSM ALLSKDHFCL CFLETEAVVG TACRQLGGHT
