WRP73_MOUSE
ID WRP73_MOUSE Reviewed; 462 AA.
AC Q9JM98; Q8BQ21;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=WD repeat-containing protein WRAP73;
DE AltName: Full=WD repeat-containing protein 8;
DE AltName: Full=WD repeat-containing protein antisense to TP73 gene;
GN Name=Wrap73; Synonyms=Dd57, Wdr8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11401440; DOI=10.1006/geno.2000.6475;
RA Koshizuka Y., Ikegawa S., Sano M., Nakamura K., Nakamura Y.;
RT "Isolation, characterization, and mapping of the mouse and human WDR8
RT genes, members of a novel WD-repeat gene family.";
RL Genomics 72:252-259(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Diencephalon, Spinal ganglion, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH SSX2IP.
RX PubMed=26675238; DOI=10.1242/jcs.179713;
RA Kurtulmus B., Wang W., Ruppert T., Neuner A., Cerikan B., Viol L.,
RA Duenas-Sanchez R., Gruss O.J., Pereira G.;
RT "WDR8 is a centriolar satellite and centriole-associated protein that
RT promotes ciliary vesicle docking during ciliogenesis.";
RL J. Cell Sci. 129:621-636(2016).
CC -!- FUNCTION: The SSX2IP:WRAP73 complex is proposed to act as regulator of
CC spindle anchoring at the mitotic centrosome. Required for the
CC centrosomal localization of SSX2IP and normal mitotic bipolar spindle
CC morphology. Required for the targeting of centriole satellite proteins
CC to centrosomes such as of PCM1, SSX2IP, CEP290 and PIBF1/CEP90.
CC Required for ciliogenesis and involved in the removal of the
CC CEP97:CCP110 complex from the mother centriole. Involved in ciliary
CC vesicle formation at the mother centriole and required for the docking
CC of vesicles to the basal body during ciliogenesis; may promote docking
CC of RAB8A- and ARL13B-containing vesicles (By similarity).
CC {ECO:0000250|UniProtKB:Q9P2S5}.
CC -!- SUBUNIT: Interacts with SSX2IP (PubMed:26675238).
CC {ECO:0000269|PubMed:26675238}.
CC -!- INTERACTION:
CC Q9JM98; Q9Y2D8: SSX2IP; Xeno; NbExp=3; IntAct=EBI-11694665, EBI-2212028;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q9P2S5}. Note=Enriched in the proximal end of
CC the mother centriole. During ciliogenesis also associated with the
CC basal body of the adjacent centriole (By similarity).
CC {ECO:0000250|UniProtKB:Q9P2S5}.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
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DR EMBL; AB034911; BAA92311.1; -; mRNA.
DR EMBL; AK051688; BAC34722.1; -; mRNA.
DR EMBL; AK156969; BAE33920.1; -; mRNA.
DR EMBL; AK160331; BAE35741.1; -; mRNA.
DR EMBL; AL806525; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466594; EDL14958.1; -; Genomic_DNA.
DR CCDS; CCDS19010.1; -.
DR RefSeq; NP_067474.2; NM_021499.2.
DR AlphaFoldDB; Q9JM98; -.
DR SMR; Q9JM98; -.
DR IntAct; Q9JM98; 2.
DR STRING; 10090.ENSMUSP00000030895; -.
DR PhosphoSitePlus; Q9JM98; -.
DR EPD; Q9JM98; -.
DR MaxQB; Q9JM98; -.
DR PaxDb; Q9JM98; -.
DR PRIDE; Q9JM98; -.
DR ProteomicsDB; 297565; -.
DR Antibodypedia; 26895; 49 antibodies from 16 providers.
DR DNASU; 59002; -.
DR Ensembl; ENSMUST00000030895; ENSMUSP00000030895; ENSMUSG00000029029.
DR GeneID; 59002; -.
DR KEGG; mmu:59002; -.
DR UCSC; uc008wbi.1; mouse.
DR CTD; 49856; -.
DR MGI; MGI:1891749; Wrap73.
DR VEuPathDB; HostDB:ENSMUSG00000029029; -.
DR eggNOG; KOG4497; Eukaryota.
DR GeneTree; ENSGT00390000003623; -.
DR HOGENOM; CLU_024072_3_1_1; -.
DR InParanoid; Q9JM98; -.
DR OMA; LAICTGN; -.
DR OrthoDB; 764000at2759; -.
DR PhylomeDB; Q9JM98; -.
DR TreeFam; TF329103; -.
DR BioGRID-ORCS; 59002; 5 hits in 73 CRISPR screens.
DR PRO; PR:Q9JM98; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9JM98; protein.
DR Bgee; ENSMUSG00000029029; Expressed in condyle and 250 other tissues.
DR ExpressionAtlas; Q9JM98; baseline and differential.
DR Genevisible; Q9JM98; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005815; C:microtubule organizing center; IBA:GO_Central.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:1990811; C:MWP complex; IBA:GO_Central.
DR GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0090307; P:mitotic spindle assembly; ISO:MGI.
DR GO; GO:1902857; P:positive regulation of non-motile cilium assembly; ISS:UniProtKB.
DR GO; GO:1902440; P:protein localization to mitotic spindle pole body; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR011044; Quino_amine_DH_bsu.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50969; SSF50969; 1.
PE 1: Evidence at protein level;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..462
FT /note="WD repeat-containing protein WRAP73"
FT /id="PRO_0000051357"
FT REPEAT 46..86
FT /note="WD 1"
FT REPEAT 89..129
FT /note="WD 2"
FT REPEAT 176..210
FT /note="WD 3"
FT REPEAT 221..260
FT /note="WD 4"
FT REPEAT 328..369
FT /note="WD 5"
FT REPEAT 371..410
FT /note="WD 6"
FT MOD_RES 281
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2S5"
FT CONFLICT 417
FT /note="P -> A (in Ref. 1; BAA92311)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 52053 MW; 6A32524BB1945845 CRC64;
MNFSESFKLS GLLCRFSPDG KYLASCVQYR LVIRDVTTLQ ILQLYTCLDQ IQHIEWSADS
LFILCAMYRR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS
LCTKSVSYIK YPKACQQGLT FTRDGRYLAL AERRDCRDYV SIFVCSDWQL LRHFDTDTQD
LTGIEWAPNG CVLAAWDTCL EYKVLLYSLD GRLLSAYCAY EWSLGIKSVA WSPSSQFLAI
GSYDGKVRLL NHVTWKMITE FGHPATINNP KTVVYKEAEK SPLLGLGHLS FPPPRAMAGA
LSTSESKYEI ASGPVSLQTL KPVADRANPR MGVGMLAFSS DSYFLASRND NVPNAVWIWD
IQKLKLFVVL EHMSPVRSFQ WDPQQPRLAI CTGGSKVYLW SPAGCVSVQV PGEGDFPVLG
LCWHLSGDSL ALLSKDHFCL CFLETKERVG TAYEQRDGMP RT
