WSB2_HUMAN
ID WSB2_HUMAN Reviewed; 404 AA.
AC Q9NYS7; B4DIE6; B4DPV6; Q9NRX9;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=WD repeat and SOCS box-containing protein 2;
DE Short=WSB-2;
DE AltName: Full=CS box-containing WD protein;
GN Name=WSB2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Colon carcinoma;
RA Li F., Song J.;
RT "cDNA cloning of a up-regulated SOCS box-containing WD protein in human
RT colonic cancer cells induced by sodium butyrate.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Zhang J.W., Liu T.X., Tao J.;
RT "Human full-length cDNA cloned from NB4 cell line induced from all trans
RT retinoid acid.";
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Hippocampus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be a substrate-recognition component of a SCF-like ECS
CC (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which
CC mediates the ubiquitination and subsequent proteasomal degradation of
CC target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9NYS7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NYS7-2; Sequence=VSP_054016;
CC Name=3;
CC IsoId=Q9NYS7-3; Sequence=VSP_054576;
CC -!- DOMAIN: The SOCS box domain mediates the interaction with the Elongin
CC BC complex, an adapter module in different E3 ubiquitin ligase
CC complexes. {ECO:0000250}.
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DR EMBL; AF229181; AAF71302.1; -; mRNA.
DR EMBL; AF163324; AAF80478.1; -; mRNA.
DR EMBL; AK295555; BAG58458.1; -; mRNA.
DR EMBL; AK298513; BAG60718.1; -; mRNA.
DR EMBL; AC131159; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC131238; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC015887; AAH15887.1; -; mRNA.
DR CCDS; CCDS61251.1; -. [Q9NYS7-3]
DR CCDS; CCDS61252.1; -. [Q9NYS7-2]
DR CCDS; CCDS9186.1; -. [Q9NYS7-1]
DR RefSeq; NP_001265486.1; NM_001278557.1. [Q9NYS7-2]
DR RefSeq; NP_001265487.1; NM_001278558.1. [Q9NYS7-3]
DR RefSeq; NP_061109.1; NM_018639.4. [Q9NYS7-1]
DR RefSeq; XP_016875131.1; XM_017019642.1. [Q9NYS7-3]
DR AlphaFoldDB; Q9NYS7; -.
DR SMR; Q9NYS7; -.
DR BioGRID; 120976; 32.
DR IntAct; Q9NYS7; 37.
DR STRING; 9606.ENSP00000409131; -.
DR PhosphoSitePlus; Q9NYS7; -.
DR BioMuta; WSB2; -.
DR DMDM; 20532294; -.
DR jPOST; Q9NYS7; -.
DR MassIVE; Q9NYS7; -.
DR PaxDb; Q9NYS7; -.
DR PeptideAtlas; Q9NYS7; -.
DR PRIDE; Q9NYS7; -.
DR ProteomicsDB; 4299; -.
DR ProteomicsDB; 4819; -.
DR ProteomicsDB; 83273; -. [Q9NYS7-1]
DR Antibodypedia; 31364; 98 antibodies from 26 providers.
DR DNASU; 55884; -.
DR Ensembl; ENST00000315436.8; ENSP00000319474.3; ENSG00000176871.9. [Q9NYS7-1]
DR Ensembl; ENST00000441406.6; ENSP00000409131.2; ENSG00000176871.9. [Q9NYS7-2]
DR Ensembl; ENST00000544233.5; ENSP00000444431.1; ENSG00000176871.9. [Q9NYS7-3]
DR GeneID; 55884; -.
DR KEGG; hsa:55884; -.
DR MANE-Select; ENST00000315436.8; ENSP00000319474.3; NM_018639.5; NP_061109.1.
DR UCSC; uc001twr.4; human. [Q9NYS7-1]
DR CTD; 55884; -.
DR DisGeNET; 55884; -.
DR GeneCards; WSB2; -.
DR HGNC; HGNC:19222; WSB2.
DR HPA; ENSG00000176871; Tissue enhanced (tongue).
DR neXtProt; NX_Q9NYS7; -.
DR OpenTargets; ENSG00000176871; -.
DR PharmGKB; PA128395787; -.
DR VEuPathDB; HostDB:ENSG00000176871; -.
DR eggNOG; KOG0266; Eukaryota.
DR GeneTree; ENSGT00890000139406; -.
DR HOGENOM; CLU_1401984_0_0_1; -.
DR InParanoid; Q9NYS7; -.
DR OMA; PWPLEEH; -.
DR OrthoDB; 805365at2759; -.
DR PhylomeDB; Q9NYS7; -.
DR TreeFam; TF329216; -.
DR PathwayCommons; Q9NYS7; -.
DR Reactome; R-HSA-8951664; Neddylation.
DR SignaLink; Q9NYS7; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 55884; 32 hits in 1124 CRISPR screens.
DR ChiTaRS; WSB2; human.
DR GenomeRNAi; 55884; -.
DR Pharos; Q9NYS7; Tbio.
DR PRO; PR:Q9NYS7; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9NYS7; protein.
DR Bgee; ENSG00000176871; Expressed in superior frontal gyrus and 209 other tissues.
DR ExpressionAtlas; Q9NYS7; baseline and differential.
DR Genevisible; Q9NYS7; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001496; SOCS_box.
DR InterPro; IPR036036; SOCS_box-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF07525; SOCS_box; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00253; SOCS; 1.
DR SMART; SM00969; SOCS_box; 1.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF158235; SSF158235; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50225; SOCS; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat; Ubl conjugation pathway;
KW WD repeat.
FT CHAIN 1..404
FT /note="WD repeat and SOCS box-containing protein 2"
FT /id="PRO_0000051460"
FT REPEAT 16..55
FT /note="WD 1"
FT REPEAT 81..140
FT /note="WD 2"
FT REPEAT 144..183
FT /note="WD 3"
FT REPEAT 188..226
FT /note="WD 4"
FT REPEAT 230..268
FT /note="WD 5"
FT REPEAT 283..322
FT /note="WD 6"
FT REPEAT 325..362
FT /note="WD 7"
FT DOMAIN 356..404
FT /note="SOCS box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00194"
FT REGION 68..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..85
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..210
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054576"
FT VAR_SEQ 1..4
FT /note="MEAG -> MRVDRESRFLRGTGTGEAVAV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054016"
FT CONFLICT 5
FT /note="E -> K (in Ref. 2; AAF80478)"
FT /evidence="ECO:0000305"
FT CONFLICT 101..103
FT /note="PWP -> LCL (in Ref. 2; AAF80478)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 404 AA; 45286 MW; 97EFC9CDE447E019 CRC64;
MEAGEEPLLL AELKPGRPHQ FDWKSSCETW SVAFSPDGSW FAWSQGHCIV KLIPWPLEEQ
FIPKGFEAKS RSSKNETKGR GSPKEKTLDC GQIVWGLAFS PWPSPPSRKL WARHHPQVPD
VSCLVLATGL NDGQIKIWEV QTGLLLLNLS GHQDVVRDLS FTPSGSLILV SASRDKTLRI
WDLNKHGKQI QVLSGHLQWV YCCSISPDCS MLCSAAGEKS VFLWSMRSYT LIRKLEGHQS
SVVSCDFSPD SALLVTASYD TNVIMWDPYT GERLRSLHHT QVDPAMDDSD VHISSLRSVC
FSPEGLYLAT VADDRLLRIW ALELKTPIAF APMTNGLCCT FFPHGGVIAT GTRDGHVQFW
TAPRVLSSLK HLCRKALRSF LTTYQVLALP IPKKMKEFLT YRTF
