WSCD1_MOUSE
ID WSCD1_MOUSE Reviewed; 572 AA.
AC Q80XH4; Q3TQ40; Q5DU24; Q8BX34;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=WSC domain-containing protein 1;
GN Name=Wscd1; Synonyms=Kiaa0523;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 521-572 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80XH4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80XH4-2; Sequence=VSP_028209, VSP_028210;
CC -!- SIMILARITY: Belongs to the WSCD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90411.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK220346; BAD90411.1; ALT_INIT; mRNA.
DR EMBL; AK049137; BAC33564.1; -; mRNA.
DR EMBL; AK163944; BAE37545.1; -; mRNA.
DR EMBL; AL672010; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC048831; AAH48831.1; -; mRNA.
DR EMBL; BC063070; AAH63070.1; -; mRNA.
DR CCDS; CCDS24975.1; -. [Q80XH4-1]
DR RefSeq; NP_808286.2; NM_177618.4. [Q80XH4-1]
DR RefSeq; XP_006532991.1; XM_006532928.3.
DR RefSeq; XP_006532992.1; XM_006532929.3. [Q80XH4-1]
DR RefSeq; XP_006532993.1; XM_006532930.3.
DR RefSeq; XP_006532994.1; XM_006532931.3. [Q80XH4-1]
DR AlphaFoldDB; Q80XH4; -.
DR SMR; Q80XH4; -.
DR STRING; 10090.ENSMUSP00000021168; -.
DR GlyGen; Q80XH4; 2 sites.
DR iPTMnet; Q80XH4; -.
DR PhosphoSitePlus; Q80XH4; -.
DR MaxQB; Q80XH4; -.
DR PaxDb; Q80XH4; -.
DR PeptideAtlas; Q80XH4; -.
DR PRIDE; Q80XH4; -.
DR ProteomicsDB; 275219; -. [Q80XH4-1]
DR ProteomicsDB; 275220; -. [Q80XH4-2]
DR Antibodypedia; 2667; 11 antibodies from 8 providers.
DR DNASU; 216881; -.
DR Ensembl; ENSMUST00000021168; ENSMUSP00000021168; ENSMUSG00000020811. [Q80XH4-1]
DR Ensembl; ENSMUST00000108510; ENSMUSP00000104150; ENSMUSG00000020811. [Q80XH4-1]
DR Ensembl; ENSMUST00000108511; ENSMUSP00000104151; ENSMUSG00000020811. [Q80XH4-1]
DR GeneID; 216881; -.
DR KEGG; mmu:216881; -.
DR UCSC; uc007jxv.1; mouse. [Q80XH4-1]
DR UCSC; uc007jxx.1; mouse. [Q80XH4-2]
DR CTD; 23302; -.
DR MGI; MGI:2448493; Wscd1.
DR VEuPathDB; HostDB:ENSMUSG00000020811; -.
DR eggNOG; KOG4157; Eukaryota.
DR GeneTree; ENSGT00940000158096; -.
DR HOGENOM; CLU_029239_1_0_1; -.
DR InParanoid; Q80XH4; -.
DR OMA; KDFEPFT; -.
DR OrthoDB; 809004at2759; -.
DR PhylomeDB; Q80XH4; -.
DR TreeFam; TF324060; -.
DR BioGRID-ORCS; 216881; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Wscd1; mouse.
DR PRO; PR:Q80XH4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q80XH4; protein.
DR Bgee; ENSMUSG00000020811; Expressed in cranial nerve II and 161 other tissues.
DR ExpressionAtlas; Q80XH4; baseline and differential.
DR Genevisible; Q80XH4; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008146; F:sulfotransferase activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000863; Sulfotransferase_dom.
DR InterPro; IPR002889; WSC_carb-bd.
DR Pfam; PF00685; Sulfotransfer_1; 1.
DR Pfam; PF01822; WSC; 2.
DR SMART; SM00321; WSC; 2.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51212; WSC; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..572
FT /note="WSC domain-containing protein 1"
FT /id="PRO_0000305062"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 139..231
FT /note="WSC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00558"
FT DOMAIN 242..337
FT /note="WSC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00558"
FT CARBOHYD 105
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 254
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 141..216
FT /note="NYLGCFSEEGQERTLKGAVFYDLRKMTVSHCQDACAERSYVYAGLEAGAECY
FT CGNRLPATRVSLKECNQECKGEKG -> SFSASATSLLRVICIVSFLGPAVAEEYDIVP
FT RSSDFDFASVTASWVTLIMSPVSGSSQVETETLTPGLLCKCIEVS (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028209"
FT VAR_SEQ 217..572
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028210"
FT CONFLICT 50
FT /note="G -> S (in Ref. 2; BAC33564)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="D -> N (in Ref. 1; BAD90411)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 572 AA; 64981 MW; D1FE48C65F5E1AA4 CRC64;
MAKPFFRLQK FLRRTQFLLL FLTAAYLMTG SLLLLQRARV ALPQALRAPG SLQALPVATV
ALGVGLLDGR SLRDPHSSPD LLLDVDTLRS PLARLPPGIR WPRRNRSSLR RRWLHHLTSD
PQGPPTLSPE ASGPANHNRG NYLGCFSEEG QERTLKGAVF YDLRKMTVSH CQDACAERSY
VYAGLEAGAE CYCGNRLPAT RVSLKECNQE CKGEKGSMCG AVRRLSVYSV GLQQPGSKKR
RTATYRGCFP LPENVTHTFS SSMTQANMTV ETCSGFCSQK EFPLAILRGW DCYCAYPTPQ
FSLRDAVDGA LCSQAPETQG LPGYCEVYQT PVQDTRCTDR KFLPDKSKVF VALSSFPGAG
NTWARHLIEH ATGFYTGSYY FDGTLYNKGF KGEKDHWRSR RTICVKTHES GRREIEMFDS
AILLIRNPYR SLVAEFNRKC AGHLGYAPDR NWKSKEWPEF VNSYASWWSS HVLDWLKYGK
RLLVVHYEEL RHSLVPTLRE MVAFLNVSVS EERLLCVENN KEGSFRRRGR RPHDQEPFTP
EMKDLINGYI RTVDQALRDH NWAGLPREYV PR
