WSCD2_MOUSE
ID WSCD2_MOUSE Reviewed; 571 AA.
AC D4PHA7; Q69ZZ2; Q8C7Q3;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=WSC domain-containing protein 2;
GN Name=Wscd2; Synonyms=Kiaa0789;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=D4PHA7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=D4PHA7-2; Sequence=VSP_042508, VSP_042509;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the WSCD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32304.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK173026; BAD32304.1; ALT_INIT; mRNA.
DR EMBL; AK049685; BAC33871.1; -; mRNA.
DR EMBL; AC121909; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01026602; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01091397; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01144085; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01166320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01167025; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CAAA01189638; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466529; EDL19945.1; -; Genomic_DNA.
DR EMBL; CH466529; EDL19946.1; -; Genomic_DNA.
DR EMBL; BC096526; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC147280; AAI47281.1; -; mRNA.
DR EMBL; BC147281; AAI47282.1; -; mRNA.
DR CCDS; CCDS19550.2; -. [D4PHA7-1]
DR RefSeq; NP_796266.2; NM_177292.3. [D4PHA7-1]
DR RefSeq; XP_017176417.1; XM_017320928.1. [D4PHA7-1]
DR AlphaFoldDB; D4PHA7; -.
DR SMR; D4PHA7; -.
DR STRING; 10090.ENSMUSP00000092021; -.
DR GlyGen; D4PHA7; 2 sites.
DR iPTMnet; D4PHA7; -.
DR PhosphoSitePlus; D4PHA7; -.
DR PaxDb; D4PHA7; -.
DR PRIDE; D4PHA7; -.
DR ProteomicsDB; 297859; -. [D4PHA7-1]
DR ProteomicsDB; 297860; -. [D4PHA7-2]
DR TopDownProteomics; D4PHA7-1; -. [D4PHA7-1]
DR Antibodypedia; 2240; 89 antibodies from 20 providers.
DR DNASU; 320916; -.
DR Ensembl; ENSMUST00000094452; ENSMUSP00000092021; ENSMUSG00000063430. [D4PHA7-1]
DR GeneID; 320916; -.
DR KEGG; mmu:320916; -.
DR UCSC; uc029vmo.1; mouse. [D4PHA7-2]
DR UCSC; uc029vmp.1; mouse. [D4PHA7-1]
DR CTD; 9671; -.
DR MGI; MGI:2445030; Wscd2.
DR VEuPathDB; HostDB:ENSMUSG00000063430; -.
DR eggNOG; KOG4157; Eukaryota.
DR GeneTree; ENSGT00940000159434; -.
DR HOGENOM; CLU_029239_0_0_1; -.
DR InParanoid; D4PHA7; -.
DR OMA; ECKGERS; -.
DR OrthoDB; 809004at2759; -.
DR PhylomeDB; D4PHA7; -.
DR TreeFam; TF324060; -.
DR BioGRID-ORCS; 320916; 3 hits in 53 CRISPR screens.
DR ChiTaRS; Wscd2; mouse.
DR PRO; PR:D4PHA7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; D4PHA7; protein.
DR Bgee; ENSMUSG00000063430; Expressed in epithelium of cochlear duct and 187 other tissues.
DR Genevisible; D4PHA7; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002889; WSC_carb-bd.
DR Pfam; PF01822; WSC; 2.
DR SMART; SM00321; WSC; 2.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51212; WSC; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..571
FT /note="WSC domain-containing protein 2"
FT /id="PRO_0000416124"
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 133..225
FT /note="WSC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00558"
FT DOMAIN 236..330
FT /note="WSC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00558"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 134..195
FT /note="AKYIGCYLDDTQSRALRGVSFFDYKKMTVFRCQDNCAERGYLYAGLEFGAEC
FT YCGHKIQAAN -> GKVLEGLRENTGTNVEITATKLVSLLQTHVNVGTLDLSSIISESH
FT SYSLIGKFHLNPTLALV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15368895,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:16141072"
FT /id="VSP_042508"
FT VAR_SEQ 196..571
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15368895,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:16141072"
FT /id="VSP_042509"
SQ SEQUENCE 571 AA; 64598 MW; 835EC3FFF086CAEB CRC64;
MAKLWFKFQR CFRYFRRKPV RFFTLLAIYL TAGSLVFLHS GFVGQPAVPQ SQASPAAGSP
VEGAELPFLG DLHLGRGFRD TVEASSIARR YGPTFKGKDT SERAKLGDYG GAWSRALKGR
VVREKEKEKE EEKAKYIGCY LDDTQSRALR GVSFFDYKKM TVFRCQDNCA ERGYLYAGLE
FGAECYCGHK IQAANVSESD CDMECKGERG SVCGGINRLS VYRLQLAQES ARRYGSAVFR
GCFRRPNNLS LALPVSAAMP NMSVDKCVDL CTEKEFPLAA LAGTACHCGF PTTRFPLHDR
EDEQLCAQKC SAEEFESCGT PSYFIVYQTQ VQDNRCMDRR FLPAKSKKLI ALASFPGAGN
TWARHLIELA TGFYTGSYYF DGSLYNKGFK GERDHWRSGR TICIKTHESG QKEIEAFDAA
ILLIRNPYKA LMAEFNRKYG GHIGFAAHAH WKGKEWPEFV RNYAPWWATH TLDWLKFGKT
VLVVHFEDLK QDLFTQLGRM VSLLGVAVRE DRLLCVESQK DGNFKRSGLR KLEYDPYTAE
MRRTIAAYIR MVDTALRSRN LTGVPDAYGP R
