WSD10_ARATH
ID WSD10_ARATH Reviewed; 483 AA.
AC Q9FK04; F4KJ46;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Wax ester synthase/diacylglycerol acyltransferase 10 {ECO:0000303|PubMed:18621978};
DE Short=WS/DGAT 10 {ECO:0000303|PubMed:18621978};
DE AltName: Full=Diacylglycerol O-acyltransferase WSD10 {ECO:0000303|PubMed:18621978};
DE EC=2.3.1.20 {ECO:0000250|UniProtKB:Q93ZR6};
DE AltName: Full=Long-chain-alcohol O-fatty-acyltransferase WSD10 {ECO:0000303|PubMed:18621978};
DE EC=2.3.1.75 {ECO:0000250|UniProtKB:Q93ZR6};
GN Name=WSD10 {ECO:0000303|PubMed:18621978};
GN OrderedLocusNames=At5g53380 {ECO:0000312|Araport:AT5G53380};
GN ORFNames=K19E1.18 {ECO:0000312|EMBL:BAB09800.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12502715; DOI=10.1074/jbc.m210533200;
RA Kalscheuer R., Steinbuchel A.;
RT "A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol
RT acyltransferase mediates wax ester and triacylglycerol biosynthesis in
RT Acinetobacter calcoaceticus ADP1.";
RL J. Biol. Chem. 278:8075-8082(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18621978; DOI=10.1104/pp.108.123471;
RA Li F., Wu X., Lam P., Bird D., Zheng H., Samuels A.L., Jetter R., Kunst L.;
RT "Identification of the wax ester synthase/acyl-coenzyme A: diacylglycerol
RT acyltransferase WSD1 required for stem wax ester biosynthesis in
RT Arabidopsis.";
RL Plant Physiol. 148:97-107(2008).
RN [5]
RP REVIEW ON ACYL-LIPID METABOLISM.
RX PubMed=23505340; DOI=10.1199/tab.0161;
RA Li-Beisson Y., Shorrosh B., Beisson F., Andersson M.X., Arondel V.,
RA Bates P.D., Baud S., Bird D., Debono A., Durrett T.P., Franke R.B.,
RA Graham I.A., Katayama K., Kelly A.A., Larson T., Markham J.E., Miquel M.,
RA Molina I., Nishida I., Rowland O., Samuels L., Schmid K.M., Wada H.,
RA Welti R., Xu C., Zallot R., Ohlrogge J.;
RT "Acyl-lipid metabolism.";
RL Arabidopsis Book 11:E0161-E0161(2013).
RN [6]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=30729606; DOI=10.1111/tpj.14269;
RA Patwari P., Salewski V., Gutbrod K., Kreszies T., Dresen-Scholz B.,
RA Peisker H., Steiner U., Meyer A.J., Schreiber L., Doermann P.;
RT "Surface wax esters contribute to drought tolerance in Arabidopsis.";
RL Plant J. 98:727-744(2019).
CC -!- FUNCTION: Bifunctional wax ester synthase/diacylglycerol
CC acyltransferase (By similarity). Involved in cuticular wax biosynthesis
CC (By similarity). {ECO:0000250|UniProtKB:Q93ZR6}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000250|UniProtKB:Q93ZR6};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000250|UniProtKB:Q93ZR6};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000250|UniProtKB:Q93ZR6}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000250|UniProtKB:Q93ZR6}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q5KS41};
CC Single-pass membrane protein {ECO:0000255}. Endoplasmic reticulum
CC membrane {ECO:0000250|UniProtKB:Q93ZR6}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots.
CC {ECO:0000269|PubMed:30729606}.
CC -!- INDUCTION: Induced in roots during drought and salt stresses.
CC {ECO:0000269|PubMed:30729606}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AED96346.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB013388; BAB09800.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96346.2; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_001318794.1; NM_001345053.1.
DR AlphaFoldDB; Q9FK04; -.
DR SMR; Q9FK04; -.
DR STRING; 3702.AT5G53380.1; -.
DR PaxDb; Q9FK04; -.
DR PRIDE; Q9FK04; -.
DR EnsemblPlants; AT5G53380.1; AT5G53380.1; AT5G53380.
DR GeneID; 835419; -.
DR Gramene; AT5G53380.1; AT5G53380.1; AT5G53380.
DR KEGG; ath:AT5G53380; -.
DR Araport; AT5G53380; -.
DR TAIR; locus:2154282; AT5G53380.
DR eggNOG; ENOG502QU8B; Eukaryota.
DR HOGENOM; CLU_027831_0_0_1; -.
DR InParanoid; Q9FK04; -.
DR OrthoDB; 828506at2759; -.
DR PhylomeDB; Q9FK04; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FK04; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102966; F:arachidoyl-CoA:1-dodecanol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0047196; F:long-chain-alcohol O-fatty-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009651; P:response to salt stress; IEP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IEP:UniProtKB.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; PTHR31650; 1.
DR Pfam; PF03007; WES_acyltransf; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Cell membrane; Endoplasmic reticulum; Glycoprotein;
KW Membrane; Reference proteome; Stress response; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..483
FT /note="Wax ester synthase/diacylglycerol acyltransferase
FT 10"
FT /id="PRO_0000452620"
FT TOPO_DOM 1..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 204..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..483
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT ACT_SITE 143
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT CARBOHYD 394
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 399
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 483 AA; 54280 MW; ABF87FCD42C9CB59 CRC64;
MTKEEVEEEP LSPMARLFQS PGIENCIITM IGFKAKINPD IILDDLKHNV SKHPRFCSKL
VIATHTNYDG ERWMKTKVNV EDHVFVPDID LQEINKDGDG FVDDYVSRLT LSPLDKSKPL
WDIHILNVKT SDAEAVGVMR CHHSLADGMS LMSLLVACTR KTSNLESFPT IPAIKRREQM
MSHRFGNKGW YSRSINAVYY AVRLIWNTIV DLLLLWATSL FFKDTETPIS EGIGSGNNAR
RFYHRTVSLD DIKLIKNAMK MTINDVLLGV TQDALSRYLN QRYGDKNGEG VTTTSNLNNL
PGKIRIRAGV AVNLRQDIGI QPLEDMLAKD SKCRWGNYDS LVFVPFSISL ETDPLVPLLK
AKSIMDRKKH SLVAPMHYSI IEFIINTFGT KVFNRTCSNT TTILSNIVGP VEEVSLHGNC
ITYIALTGYG HSQALMIHFI SYANKMIITI AVDPAVIPDP HNICDEMEKS LKAMKDTLSG
KSD
