WSDU1_HUMAN
ID WSDU1_HUMAN Reviewed; 476 AA.
AC Q8N9V3; Q53TI9; Q8N6N8;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 4.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=WD repeat, SAM and U-box domain-containing protein 1;
GN Name=WDSUB1; Synonyms=WDSAM1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT SER-320.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ASP-223.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-458, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N9V3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N9V3-2; Sequence=VSP_023337;
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DR EMBL; AK093494; BAC04184.1; -; mRNA.
DR EMBL; AC008277; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC009307; AAX93043.1; -; Genomic_DNA.
DR EMBL; BC029520; AAH29520.1; -; mRNA.
DR CCDS; CCDS2208.1; -. [Q8N9V3-1]
DR CCDS; CCDS77477.1; -. [Q8N9V3-2]
DR RefSeq; NP_001121684.1; NM_001128212.2.
DR RefSeq; NP_001294923.1; NM_001307994.1.
DR RefSeq; NP_689741.2; NM_152528.2.
DR AlphaFoldDB; Q8N9V3; -.
DR SMR; Q8N9V3; -.
DR BioGRID; 127386; 2.
DR STRING; 9606.ENSP00000387078; -.
DR iPTMnet; Q8N9V3; -.
DR PhosphoSitePlus; Q8N9V3; -.
DR BioMuta; WDSUB1; -.
DR DMDM; 229463016; -.
DR EPD; Q8N9V3; -.
DR jPOST; Q8N9V3; -.
DR MassIVE; Q8N9V3; -.
DR MaxQB; Q8N9V3; -.
DR PaxDb; Q8N9V3; -.
DR PeptideAtlas; Q8N9V3; -.
DR PRIDE; Q8N9V3; -.
DR ProteomicsDB; 72588; -. [Q8N9V3-1]
DR ProteomicsDB; 72589; -. [Q8N9V3-2]
DR Antibodypedia; 33718; 113 antibodies from 20 providers.
DR DNASU; 151525; -.
DR Ensembl; ENST00000358147.8; ENSP00000350866.4; ENSG00000196151.11. [Q8N9V3-2]
DR Ensembl; ENST00000359774.9; ENSP00000352820.4; ENSG00000196151.11. [Q8N9V3-1]
DR Ensembl; ENST00000392796.7; ENSP00000376545.3; ENSG00000196151.11. [Q8N9V3-1]
DR Ensembl; ENST00000409990.7; ENSP00000387078.3; ENSG00000196151.11. [Q8N9V3-1]
DR GeneID; 151525; -.
DR KEGG; hsa:151525; -.
DR MANE-Select; ENST00000359774.9; ENSP00000352820.4; NM_001128212.3; NP_001121684.1.
DR UCSC; uc002uaj.5; human. [Q8N9V3-1]
DR CTD; 151525; -.
DR GeneCards; WDSUB1; -.
DR HGNC; HGNC:26697; WDSUB1.
DR HPA; ENSG00000196151; Low tissue specificity.
DR neXtProt; NX_Q8N9V3; -.
DR OpenTargets; ENSG00000196151; -.
DR PharmGKB; PA128394761; -.
DR VEuPathDB; HostDB:ENSG00000196151; -.
DR eggNOG; KOG4155; Eukaryota.
DR GeneTree; ENSGT00940000157230; -.
DR HOGENOM; CLU_038099_0_0_1; -.
DR InParanoid; Q8N9V3; -.
DR PhylomeDB; Q8N9V3; -.
DR TreeFam; TF328991; -.
DR PathwayCommons; Q8N9V3; -.
DR SignaLink; Q8N9V3; -.
DR BioGRID-ORCS; 151525; 14 hits in 1128 CRISPR screens.
DR ChiTaRS; WDSUB1; human.
DR GenomeRNAi; 151525; -.
DR Pharos; Q8N9V3; Tdark.
DR PRO; PR:Q8N9V3; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N9V3; protein.
DR Bgee; ENSG00000196151; Expressed in oviduct epithelium and 183 other tissues.
DR ExpressionAtlas; Q8N9V3; baseline and differential.
DR Genevisible; Q8N9V3; HS.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR Gene3D; 1.10.150.50; -; 1.
DR Gene3D; 2.130.10.10; -; 3.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF07647; SAM_2; 1.
DR Pfam; PF04564; U-box; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00454; SAM; 1.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF47769; SSF47769; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..476
FT /note="WD repeat, SAM and U-box domain-containing protein
FT 1"
FT /id="PRO_0000278608"
FT REPEAT 10..47
FT /note="WD 1"
FT REPEAT 52..91
FT /note="WD 2"
FT REPEAT 95..134
FT /note="WD 3"
FT REPEAT 137..176
FT /note="WD 4"
FT REPEAT 178..228
FT /note="WD 5"
FT REPEAT 237..276
FT /note="WD 6"
FT REPEAT 279..318
FT /note="WD 7"
FT DOMAIN 332..396
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT DOMAIN 403..476
FT /note="U-box"
FT MOD_RES 458
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT VAR_SEQ 226..317
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023337"
FT VARIANT 215
FT /note="K -> T (in dbSNP:rs16843852)"
FT /id="VAR_030791"
FT VARIANT 223
FT /note="H -> D (in dbSNP:rs17852677)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030792"
FT VARIANT 320
FT /note="R -> S (in dbSNP:rs7591849)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_030793"
SQ SEQUENCE 476 AA; 52833 MW; 457C61236A8B6F78 CRC64;
MVKLIHTLAD HGDDVNCCAF SFSLLATCSL DKTIRLYSLR DFTELPHSPL KFHTYAVHCC
CFSPSGHILA SCSTDGTTVL WNTENGQMLA VMEQPSGSPV RVCQFSPDST CLASGAADGT
VVLWNAQSYK LYRCGSVKDG SLAACAFSPN GSFFVTGSSC GDLTVWDDKM RCLHSEKAHD
LGITCCDFSS QPVSDGEQGL QFFRLASCGQ DCQVKIWIVS FTHILGFELK YKSTLSGHCA
PVLACAFSHD GQMLVSGSVD KSVIVYDTNT ENILHTLTQH TRYVTTCAFA PNTLLLATGS
MDKTVNIWQF DLETLCQARR TEHQLKQFTE DWSEEDVSTW LCAQDLKDLV GIFKMNNIDG
KELLNLTKES LADDLKIESL GLRSKVLRKI EELRTKVKSL SSGIPDEFIC PITRELMKDP
VIASDGYSYE KEAMENWISK KKRTSPMTNL VLPSAVLTPN RTLKMAINRW LETHQK
