WTF15_SCHPO
ID WTF15_SCHPO Reviewed; 255 AA.
AC G2TRU7;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=Wtf element wtf15 {ECO:0000312|PomBase:SPCC663.17};
GN Name=wtf15 {ECO:0000312|PomBase:SPCC663.17};
GN ORFNames=SPCC663.17 {ECO:0000312|PomBase:SPCC663.17};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP LACK OF ALTERNATIVE SPLICING.
RX PubMed=30991417; DOI=10.1093/molbev/msz052;
RA Eickbush M.T., Young J.M., Zanders S.E.;
RT "Killer Meiotic Drive and Dynamic Evolution of the wtf Gene Family.";
RL Mol. Biol. Evol. 36:1201-1214(2019).
RN [4]
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA Kent A.M., Zanders S.E.;
RT "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT display high gamete-killing efficiency.";
RL PLoS Genet. 16:e1008350-e1008350(2020).
CC -!- FUNCTION: May act in meiotic drive. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- SUBCELLULAR LOCATION: Spore membrane {ECO:0000250|UniProtKB:A0A482AT42,
CC ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Present following meiosis I (at protein level).
CC {ECO:0000269|PubMed:32032353}.
CC -!- DISRUPTION PHENOTYPE: Simultaneous knockout of wtf14 results in normal
CC vegetative cell population growth (PubMed:32032353). Simultaneous
CC knockout of wtf14, wtf11 and wtf7 results in normal spore viability
CC (PubMed:32032353). {ECO:0000269|PubMed:32032353}.
CC -!- MISCELLANEOUS: Although the gene is capable of encoding two isoforms,
CC RNA sequencing data show no evidence of alternative transcripts.
CC {ECO:0000305|PubMed:30991417}.
CC -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
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DR EMBL; CU329672; CAO77692.2; -; Genomic_DNA.
DR RefSeq; XP_004001750.1; XM_004001701.1.
DR AlphaFoldDB; G2TRU7; -.
DR SMR; G2TRU7; -.
DR BioGRID; 280193; 16.
DR STRING; 4896.SPCC663.17.1; -.
DR PaxDb; G2TRU7; -.
DR EnsemblFungi; SPCC663.17.1; SPCC663.17.1:pep; SPCC663.17.
DR PomBase; SPCC663.17; wtf15.
DR VEuPathDB; FungiDB:SPCC663.17; -.
DR HOGENOM; CLU_092895_0_0_1; -.
DR PRO; PR:G2TRU7; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0110134; P:meiotic drive; ISM:PomBase.
DR InterPro; IPR004982; WTF.
DR Pfam; PF03303; WTF; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..255
FT /note="Wtf element wtf15"
FT /id="PRO_0000416675"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 19..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 255 AA; 28404 MW; AFA41DF87805D88C CRC64;
MSNNYTSLSS SLDEEMGLKA GHEIDLEGSP PSEHNSEEKS TLPSNSDILT SANPVSQASE
TPDHSIESNT GSTQSPTSHS LLLKFSFCIV YYSYFAIVVL GCVLPFEHTH TFLIAFLVIF
GIISVILFSG SIYYYETWTK TVKHFLKKVI SPFKKEYIVC AFLKTFVFYG LLKTIEHFLV
LLSGDKWGWK CSTLSSILTP VSCISFCLNE SVQLRSCSTH LFINTVAWIK SLGGGKNAFE
NNYNQLNETS PEDLV
