WTF19_SCHPO
ID WTF19_SCHPO Reviewed; 393 AA.
AC O74486; P78881;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Meiotic driver wtf19 {ECO:0000303|PubMed:32032353};
GN Name=wtf19 {ECO:0000312|PomBase:SPCC1906.03};
GN ORFNames=SPCC1906.03 {ECO:0000312|PomBase:SPCC1906.03};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 105-393.
RC STRAIN=PR745;
RX PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT "Identification of open reading frames in Schizosaccharomyces pombe
RT cDNAs.";
RL DNA Res. 4:363-369(1997).
RN [3]
RP ALTERNATIVE INITIATION (ISOFORMS 1 AND 2).
RX PubMed=30991417; DOI=10.1093/molbev/msz052;
RA Eickbush M.T., Young J.M., Zanders S.E.;
RT "Killer Meiotic Drive and Dynamic Evolution of the wtf Gene Family.";
RL Mol. Biol. Evol. 36:1201-1214(2019).
RN [4]
RP FUNCTION.
RX PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA Kent A.M., Zanders S.E.;
RT "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT display high gamete-killing efficiency.";
RL PLoS Genet. 16:e1008350-e1008350(2020).
CC -!- FUNCTION: Promotes unequal transmission of alleles from the parental
CC zygote to progeny spores by acting as poison/antidote system where the
CC poison and antidote proteins are produced from the same locus; the
CC poison component is trans-acting and targets all spores within an ascus
CC whereas the antidote component is spore-specific, leading to poisoning
CC of all progeny that do not inherit the allele.
CC {ECO:0000269|PubMed:32032353}.
CC -!- FUNCTION: [Isoform 1]: Localizes isoform 2 to the vacuole thereby
CC facilitating its degradation. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- FUNCTION: [Isoform 2]: Forms toxic aggregates that disrupt spore
CC maturation. {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- SUBUNIT: Homomer (By similarity). Forms protein aggregates (By
CC similarity). The two isoforms can interact with each other and with
CC themselves (By similarity). High sequence similarity is required for
CC their interaction (By similarity). {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000250|UniProtKB:O74420}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Spore membrane
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Vacuole membrane
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Note=Contained within spores expressing the
CC isoform and localizes isoform 2 to the vacuole.
CC {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Ascus epiplasm
CC {ECO:0000250|UniProtKB:A0A218N034}. Cytoplasm
CC {ECO:0000250|UniProtKB:A0A218N034}. Spore membrane
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Vacuole membrane
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Note=Localizes in trans to all spores within an
CC ascus. Localization to the spore vacuole is dependent on isoform 1.
CC {ECO:0000250|UniProtKB:A0A218N034}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1; Synonyms=Antidote {ECO:0000305}, Suppressor {ECO:0000305};
CC IsoId=O74486-1; Sequence=Displayed;
CC Name=2; Synonyms=Poison {ECO:0000305};
CC IsoId=O74486-2; Sequence=VSP_060944;
CC -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA13893.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; CU329672; CAA20772.1; -; Genomic_DNA.
DR EMBL; D89232; BAA13893.1; ALT_FRAME; mRNA.
DR PIR; T41211; T41211.
DR PIR; T43136; T43136.
DR RefSeq; NP_588407.1; NM_001023398.2.
DR AlphaFoldDB; O74486; -.
DR BioGRID; 275827; 2.
DR STRING; 4896.SPCC1906.03.1; -.
DR PaxDb; O74486; -.
DR EnsemblFungi; SPCC1906.03.1; SPCC1906.03.1:pep; SPCC1906.03. [O74486-1]
DR GeneID; 2539257; -.
DR KEGG; spo:SPCC1906.03; -.
DR PomBase; SPCC1906.03; wtf19.
DR VEuPathDB; FungiDB:SPCC1906.03; -.
DR HOGENOM; CLU_763247_0_0_1; -.
DR PhylomeDB; O74486; -.
DR PRO; PR:O74486; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; HDA:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0110134; P:meiotic drive; IDA:UniProtKB.
DR InterPro; IPR004982; WTF.
DR Pfam; PF03303; WTF; 2.
PE 2: Evidence at transcript level;
KW Alternative initiation; Cytoplasm; Endoplasmic reticulum; Membrane;
KW Reference proteome; Toxin; Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..393
FT /note="Meiotic driver wtf19"
FT /id="PRO_0000193231"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 15..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..55
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000250|UniProtKB:A0A218N035"
FT /id="VSP_060944"
FT CONFLICT 183
FT /note="L -> S (in Ref. 2; BAA13893)"
FT /evidence="ECO:0000305"
FT CONFLICT 243
FT /note="L -> F (in Ref. 2; BAA13893)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 393 AA; 43945 MW; 933049A43EE1D39B CRC64;
MKNKYYPLRS SMDELSAKND NEIDLEKGPL PEYNSEDGST LPPYSENINL KDPKQMGANN
PNLFNTDEST TPPDYGEDSL SITHRENHSS GTADNSSTSP LKKAFLSFIS IFVLNVPAVC
YLTYKDALFK DYGKDEWVYF AVWCASCLMI FISLWYFYET WIKAVKVTVI FLAQCIKVTV
VFLAQCVKVT SISLAKCVKL TAVFLAQCVK VTAVFLAQCV KVISIGLFNI RREMMIIIWL
LWLIICCILF GCVKSGDLNL NKALIYSTCT ISAVLLLIVS SVCIPFWTFE RTLAKLAKVF
LLQSGIVLVL NGTMFLRGKH FEWTGCEIEA SVLFIMGNVL FLCEMECPGA LIRTRNSIRN
GIAFILEGAG RAIRGANDNN DIPLGEMEVE SEV
