WTF1_MAIZE
ID WTF1_MAIZE Reviewed; 498 AA.
AC B6TTV8;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Protein WHAT'S THIS FACTOR 1, chloroplastic {ECO:0000303|PubMed:19251672};
DE Flags: Precursor;
GN Name=WTF1 {ECO:0000303|PubMed:19251672};
GN ORFNames=ZEAMMB73_Zm00001d009618 {ECO:0000312|EMBL:AQK92309.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. B73;
RX PubMed=19251672; DOI=10.1073/pnas.0812503106;
RA Kroeger T.S., Watkins K.P., Friso G., van Wijk K.J., Barkan A.;
RT "A plant-specific RNA-binding domain revealed through analysis of
RT chloroplast group II intron splicing.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:4537-4542(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
CC -!- FUNCTION: RNA-binding protein involved in the chloroplastic group II
CC intron splicing. Binds specific group II introns and promotes their
CC splicing. Functions in the context of a heterodimer with the
CC ribonuclease III domain-containing protein RNC1.
CC {ECO:0000269|PubMed:18937034}.
CC -!- INTERACTION:
CC B6TTV8; Q84N49: CAF1; NbExp=3; IntAct=EBI-15761679, EBI-15761664;
CC B6TTV8; Q84N48: CAF2; NbExp=3; IntAct=EBI-15761679, EBI-15761721;
CC B6TTV8; A6YSL1: RNC1; NbExp=4; IntAct=EBI-15761679, EBI-15761735;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19251672}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethality.
CC {ECO:0000269|PubMed:19251672}.
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DR EMBL; FJ264201; ACI96105.1; -; Genomic_DNA.
DR EMBL; CM000784; AQK92309.1; -; Genomic_DNA.
DR EMBL; EU968423; ACG40541.1; -; mRNA.
DR RefSeq; NP_001144434.1; NM_001150962.2.
DR AlphaFoldDB; B6TTV8; -.
DR DIP; DIP-48739N; -.
DR IntAct; B6TTV8; 5.
DR STRING; 4577.GRMZM2G403797_P01; -.
DR PRIDE; B6TTV8; -.
DR EnsemblPlants; Zm00001eb344150_T001; Zm00001eb344150_P001; Zm00001eb344150.
DR GeneID; 100277394; -.
DR Gramene; Zm00001eb344150_T001; Zm00001eb344150_P001; Zm00001eb344150.
DR KEGG; zma:100277394; -.
DR eggNOG; ENOG502QRW0; Eukaryota.
DR OMA; SPYSDFT; -.
DR OrthoDB; 710613at2759; -.
DR Proteomes; UP000007305; Chromosome 8.
DR ExpressionAtlas; B6TTV8; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0000373; P:Group II intron splicing; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IMP:UniProtKB.
DR InterPro; IPR021099; PORR_domain.
DR InterPro; IPR045040; PORR_fam.
DR InterPro; IPR040293; WTF1-like.
DR PANTHER; PTHR31476; PTHR31476; 1.
DR PANTHER; PTHR31476:SF4; PTHR31476:SF4; 1.
DR Pfam; PF11955; PORR; 1.
PE 1: Evidence at protein level;
KW Chloroplast; mRNA processing; mRNA splicing; Plastid; Reference proteome;
KW RNA-binding; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..498
FT /note="Protein WHAT'S THIS FACTOR 1, chloroplastic"
FT /id="PRO_0000441884"
FT DOMAIN 59..387
FT /note="PORR"
FT /evidence="ECO:0000255"
FT REGION 397..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 446..498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 498 AA; 56980 MW; 07929BB02BE2FCF9 CRC64;
MDAKLLLPFP FAPAAATRSP KSLFLGAPLP PPPRPPPFPL RLRPRPAAVV AQAAVKRRKE
APFDTVIQRD KKLKLVLKLR NILVAQPDRV MSLRELGRFR RDLGLTRKRR LIALLRRFPG
VFDVVEEGVY SLRFRLTPAA ERLYLDELRL RNESEGLAVA KLRKLLMMSQ EKRILIEKVA
HLKHDLGLPP EFRDTVCLRY PQYFRVVRMD RGPALELTHW DPELAVSAAE LAEEESRARE
AEERNLIIDR PLKFNRVRLP KGLKLTRGEA RRIARFKEMP YISPYADFSH LRSGSDEKEK
HACGVVHEIL SLTVEKRTLV DHLTHFREEF RFSQSLRGMI IRHPDMFYVS FKGDRDSVFL
REAYKDSQLV EKNQLVLLKE KMRALVAVPR FPRRAAVGTG EEAEGMNGSL QSRDQVSDEE
YDDEDEGLSD MEDLISELSG GKSDADYEWG DGWFGENDDS PPDFGDDEVK VAMKIADGSA
NGSAPVPVFP DGRPRERW
