WTF20_SCHPO
ID WTF20_SCHPO Reviewed; 258 AA.
AC O74487;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Meiotic drive suppressor wtf20 {ECO:0000312|PomBase:SPCC1906.04};
GN Name=wtf20 {ECO:0000312|PomBase:SPCC1906.04}; Synonyms=wtf6;
GN ORFNames=SPCC1906.04 {ECO:0000312|PomBase:SPCC1906.04};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=32032353; DOI=10.1371/journal.pgen.1008350;
RA Bravo Nunez M.A., Sabbarini I.M., Eickbush M.T., Liang Y., Lange J.J.,
RA Kent A.M., Zanders S.E.;
RT "Dramatically diverse Schizosaccharomyces pombe wtf meiotic drivers all
RT display high gamete-killing efficiency.";
RL PLoS Genet. 16:e1008350-e1008350(2020).
CC -!- FUNCTION: Acts as a suppressor component of the dual wtf meiotic drive
CC system, and can suppress but not confer meiotic drive by compatible
CC poisons (By similarity). Wtf meiotic drive systems promote unequal
CC transmission of alleles from the parental zygote to progeny spores by
CC encoding a poison and an antidote from the same locus; the poison is
CC trans-acting and forms toxic aggregates in all spores within an ascus,
CC wherease the antidote is spore-specific and targets aggregates for
CC degradation by the vacuole (By similarity). Meiotic drive by wtf
CC systems therefore lead to poisoning of all progeny that do not inherit
CC the dual poison/antidote allele, or express a compatible antidote (By
CC similarity). {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000250|UniProtKB:A0A482ATU4}.
CC -!- SUBUNIT: Homomer (By similarity). Interacts with other proteins that
CC exhibit high sequence similarity (By similarity).
CC {ECO:0000250|UniProtKB:A0A218N034, ECO:0000250|UniProtKB:O74420}.
CC -!- SUBCELLULAR LOCATION: Spore membrane {ECO:0000250|UniProtKB:A0A218N034,
CC ECO:0000255}; Multi-pass membrane protein {ECO:0000255}. Vacuole
CC membrane {ECO:0000250|UniProtKB:A0A218N034, ECO:0000255}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Progressively increases throughout meiosis (at
CC protein level). {ECO:0000269|PubMed:32032353}.
CC -!- SIMILARITY: Belongs to the WTF family. {ECO:0000305}.
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DR EMBL; CU329672; CAA20773.1; -; Genomic_DNA.
DR PIR; T41212; T41212.
DR RefSeq; NP_588408.1; NM_001023399.2.
DR AlphaFoldDB; O74487; -.
DR BioGRID; 275906; 6.
DR STRING; 4896.SPCC1906.04.1; -.
DR PaxDb; O74487; -.
DR EnsemblFungi; SPCC1906.04.1; SPCC1906.04.1:pep; SPCC1906.04.
DR GeneID; 2539340; -.
DR KEGG; spo:SPCC1906.04; -.
DR PomBase; SPCC1906.04; wtf20.
DR VEuPathDB; FungiDB:SPCC1906.04; -.
DR HOGENOM; CLU_092895_0_0_1; -.
DR PhylomeDB; O74487; -.
DR PRO; PR:O74487; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005737; C:cytoplasm; ISS:PomBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0110134; P:meiotic drive; ISM:PomBase.
DR InterPro; IPR004982; WTF.
DR Pfam; PF03303; WTF; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..258
FT /note="Meiotic drive suppressor wtf20"
FT /id="PRO_0000193232"
FT TRANSMEM 84..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..68
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 258 AA; 29185 MW; 63876DD8558F50B4 CRC64;
MKNNYTSLKS PLDEEDELKT DHEIDLEKGL LPEYNSEEEG ALPPYSDISK LANPVPEDSS
TGPTEIANPN VERRQEFKDS HPNIYFLLRL LISVLAVSVV FFTAWVCVNP LEKSIFGKVA
FSVTIGITCP ILFIAIFYFY ETWTKACGKG IKHFLKKWRN MFFTFCKSPI FCLVLLKAEN
KLSSHLGDQQ WGWKCSASAF TFMAVSSILI FIAETVEPGS CSTDLVKRVQ AYCDYEARQY
ASSNTAIPLR EMNPENEA
